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Article: Stimulation of ecto-5′-nucleotidase in human umbilical vein endothelial cells by lipopolysaccharide

TitleStimulation of ecto-5′-nucleotidase in human umbilical vein endothelial cells by lipopolysaccharide
Authors
KeywordsAdenosine
Cytokines
Inflammation
Issue Date2008
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/
Citation
American Journal Of Physiology - Heart And Circulatory Physiology, 2008, v. 295 n. 3, p. H1177-H1181 How to Cite?
AbstractThe involvement of ecto-5′-nucleotidase (E-5′Nu) in the elevation of extracellular adenosine during inflammation is unclear. In the present study, the effect of lipopolysaccharide (LPS), an inflammation inducer, was investigated on E-5′Nu in human umbilical vein endothelial cells (HUVECs). E-5′Nu activity was enhanced after a 24 h exposure to LPS. This effect was dose dependent, with an EC 50 of 1.66 ng/ml. At 10 μM, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY-294002 abolished the LPS-induced E-5′Nu activity. However, at 10 μM, the NF-κB inhibitor ammonium pyrrolidine dithiocarbamate had no effect. LPS upregulated the protein expression but not the messenger RNA expression of E-5′Nu. The inhibition of E-5′Nu by 100 μM α,β-methylene adenosine-5′-diphosphate increased the LPS-induced inflammation, suggesting that E-5′Nu plays a significant role in reducing inflammation, probably through the generation of adenosine. In conclusion, the experiments indicate that LPS upregulates E-5′Nu activity in HUVECs through a PI3K-dependent increase in the abundance of E-5′Nu on cell membranes. Since adenosine is an anti-inflammatory molecule, E-5′Nu upregulation may be crucial in protecting endothelial cells against inflammatory damage. Copyright © 2008 the American Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/171372
ISSN
2015 Impact Factor: 3.324
2015 SCImago Journal Rankings: 1.823
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, RWSen_US
dc.contributor.authorMan, RYKen_US
dc.contributor.authorVanhoutte, PMen_US
dc.contributor.authorLeung, GPHen_US
dc.date.accessioned2012-10-30T06:13:40Z-
dc.date.available2012-10-30T06:13:40Z-
dc.date.issued2008en_US
dc.identifier.citationAmerican Journal Of Physiology - Heart And Circulatory Physiology, 2008, v. 295 n. 3, p. H1177-H1181en_US
dc.identifier.issn0363-6135en_US
dc.identifier.urihttp://hdl.handle.net/10722/171372-
dc.description.abstractThe involvement of ecto-5′-nucleotidase (E-5′Nu) in the elevation of extracellular adenosine during inflammation is unclear. In the present study, the effect of lipopolysaccharide (LPS), an inflammation inducer, was investigated on E-5′Nu in human umbilical vein endothelial cells (HUVECs). E-5′Nu activity was enhanced after a 24 h exposure to LPS. This effect was dose dependent, with an EC 50 of 1.66 ng/ml. At 10 μM, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY-294002 abolished the LPS-induced E-5′Nu activity. However, at 10 μM, the NF-κB inhibitor ammonium pyrrolidine dithiocarbamate had no effect. LPS upregulated the protein expression but not the messenger RNA expression of E-5′Nu. The inhibition of E-5′Nu by 100 μM α,β-methylene adenosine-5′-diphosphate increased the LPS-induced inflammation, suggesting that E-5′Nu plays a significant role in reducing inflammation, probably through the generation of adenosine. In conclusion, the experiments indicate that LPS upregulates E-5′Nu activity in HUVECs through a PI3K-dependent increase in the abundance of E-5′Nu on cell membranes. Since adenosine is an anti-inflammatory molecule, E-5′Nu upregulation may be crucial in protecting endothelial cells against inflammatory damage. Copyright © 2008 the American Physiological Society.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Heart and Circulatory Physiologyen_US
dc.subjectAdenosineen_US
dc.subjectCytokinesen_US
dc.subjectInflammationen_US
dc.titleStimulation of ecto-5′-nucleotidase in human umbilical vein endothelial cells by lipopolysaccharideen_US
dc.typeArticleen_US
dc.identifier.emailMan, RYK:rykman@hkucc.hku.hken_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.emailLeung, GPH:gphleung@hkucc.hku.hken_US
dc.identifier.authorityMan, RYK=rp00236en_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.identifier.authorityLeung, GPH=rp00234en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1152/ajpheart.91513.2007en_US
dc.identifier.pmid18641267-
dc.identifier.scopuseid_2-s2.0-54049123058en_US
dc.identifier.hkuros147882-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-54049123058&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume295en_US
dc.identifier.issue3en_US
dc.identifier.spageH1177en_US
dc.identifier.epageH1181en_US
dc.identifier.eissn1522-1539-
dc.identifier.isiWOS:000258949200035-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLi, RWS=7404722884en_US
dc.identifier.scopusauthoridMan, RYK=7004986435en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US
dc.identifier.scopusauthoridLeung, GPH=35963668200en_US

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