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Article: Expression and activity of cyclooxygenases-1 and -2 in acetic acid induced gastric ulcer in rats

TitleExpression and activity of cyclooxygenases-1 and -2 in acetic acid induced gastric ulcer in rats
Authors
Issue Date2001
Publisher中華醫學會. The Journal's web site is located at http://zhyx.chinajournal.net.cn/
Citation
Zhonghua Yi Xue Za Zhi., 2001, v. 81 n. 24, p. 1519-1522 How to Cite?
AbstractOBJECTIVE: To investigate the dynamic changes of the expression and activity of the two forms of cyclooxygenase (COX-1 and COX-2) in animal model of gastric ulcer and their role in the pathological process of gastric ulcer. METHODS: Acetic acid was perfused into the gastric cavitties of 130 rats to produce animal model of /gastric ulcer. Specimens of the base part and margin of gastric ulcer and normal gastric tissue around were collected 1 hour to 15 days after the induction. RT-PCR and Western blotting were used to detect the expression of COX-1 mRNA and COX-2 mRNA and their protein levels. The total COX activity was determined by determining the ability of tissue homogenate to metabolize arachidonic acid to produce PGE2. RESULTS: After the ulcer induction, the expression of COX-2 mRNA and its protein were highly induced at the ulcer base in inflammation stage (6 hours to 3 days) and kept at a high level during the healing stage (3 days to 15 days), while the expression of COX-1 remained relatively stable. The homogenates from tissues of basal and marginal parts of ulcer showed very high level of COX activity during the ulcer healing stage. The COX activity of normal tissue around ulcer showed little change before and after ulcer induction. CONCLUSION: COX, especially COX-2, which maintains a high level of expression and activity during the ulcer healing stage, may play an important role in ulcer healing and tissue remodeling.
Persistent Identifierhttp://hdl.handle.net/10722/171339
ISSN
2015 SCImago Journal Rankings: 0.134

 

DC FieldValueLanguage
dc.contributor.authorGuo, Jen_US
dc.contributor.authorKoo, MWen_US
dc.contributor.authorWang, Jen_US
dc.date.accessioned2012-10-30T06:13:28Z-
dc.date.available2012-10-30T06:13:28Z-
dc.date.issued2001en_US
dc.identifier.citationZhonghua Yi Xue Za Zhi., 2001, v. 81 n. 24, p. 1519-1522en_US
dc.identifier.issn0376-2491en_US
dc.identifier.urihttp://hdl.handle.net/10722/171339-
dc.description.abstractOBJECTIVE: To investigate the dynamic changes of the expression and activity of the two forms of cyclooxygenase (COX-1 and COX-2) in animal model of gastric ulcer and their role in the pathological process of gastric ulcer. METHODS: Acetic acid was perfused into the gastric cavitties of 130 rats to produce animal model of /gastric ulcer. Specimens of the base part and margin of gastric ulcer and normal gastric tissue around were collected 1 hour to 15 days after the induction. RT-PCR and Western blotting were used to detect the expression of COX-1 mRNA and COX-2 mRNA and their protein levels. The total COX activity was determined by determining the ability of tissue homogenate to metabolize arachidonic acid to produce PGE2. RESULTS: After the ulcer induction, the expression of COX-2 mRNA and its protein were highly induced at the ulcer base in inflammation stage (6 hours to 3 days) and kept at a high level during the healing stage (3 days to 15 days), while the expression of COX-1 remained relatively stable. The homogenates from tissues of basal and marginal parts of ulcer showed very high level of COX activity during the ulcer healing stage. The COX activity of normal tissue around ulcer showed little change before and after ulcer induction. CONCLUSION: COX, especially COX-2, which maintains a high level of expression and activity during the ulcer healing stage, may play an important role in ulcer healing and tissue remodeling.en_US
dc.languageengen_US
dc.publisher中華醫學會. The Journal's web site is located at http://zhyx.chinajournal.net.cn/en_US
dc.relation.ispartofZhonghua yi xue za zhi.en_US
dc.subject.meshAcetic Aciden_US
dc.subject.meshAnimalsen_US
dc.subject.meshCyclooxygenase 1 - Biosynthesis - Geneticsen_US
dc.subject.meshCyclooxygenase 2 - Biosynthesis - Geneticsen_US
dc.subject.meshIsoenzymes - Biosynthesis - Geneticsen_US
dc.subject.meshMaleen_US
dc.subject.meshRna, Messenger - Biosynthesis - Geneticsen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshStomach Ulcer - Chemically Induced - Enzymologyen_US
dc.titleExpression and activity of cyclooxygenases-1 and -2 in acetic acid induced gastric ulcer in ratsen_US
dc.typeArticleen_US
dc.identifier.emailKoo, MW:wlkoo@hku.hken_US
dc.identifier.authorityKoo, MW=rp00233en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid16200780-
dc.identifier.scopuseid_2-s2.0-33645921955en_US
dc.identifier.volume81en_US
dc.identifier.issue24en_US
dc.identifier.spage1519en_US
dc.identifier.epage1522en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridGuo, J=7404488815en_US
dc.identifier.scopusauthoridKoo, MW=7004550899en_US
dc.identifier.scopusauthoridWang, J=7701327349en_US

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