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Article: Bioassay of endothelium-derived relaxing factor(s): inactivation by catecholamines.

TitleBioassay of endothelium-derived relaxing factor(s): inactivation by catecholamines.
Authors
Issue Date1985
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
The American Journal Of Physiology, 1985, v. 249 n. 1 Pt 2, p. H95-101 How to Cite?
AbstractA bioassay technique was developed to analyze the effect of vasoactive substance(s) released from endothelial cells. Canine femoral arteries with or without endothelium were perfused with physiological salt solution at 37 degrees C. The perfusate was bioassayed with a ring of coronary artery without endothelium. A substance(s) released by the endothelial cells under basal conditions caused relaxation of unstimulated coronary arteries or relaxation of those contracted with prostaglandin F2 alpha. The release of the relaxing substance(s) was augmented by acetylcholine. The relaxation induced by acetylcholine was biphasic: an initial rapid phase followed by a partial recovery and a slowly developing prolonged relaxation; the half-life of the substance(s) causing the initial phase averaged 6.3 s. Norepinephrine, epinephrine, and ascorbic acid, given downstream of the femoral artery, reversibly prevented the second phase but only attenuated the initial relaxation. These observations indicate that an endothelium-derived relaxing substance(s) is released into the lumen of the femoral artery under basal conditions and during stimulation with acetylcholine. Catecholamines can inactivate the relaxing substance(s) but do not prevent either its production by endothelial cells or its action on vascular smooth muscle.
Persistent Identifierhttp://hdl.handle.net/10722/171310
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRubanyi, GMen_US
dc.contributor.authorLorenz, RRen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:13:19Z-
dc.date.available2012-10-30T06:13:19Z-
dc.date.issued1985en_US
dc.identifier.citationThe American Journal Of Physiology, 1985, v. 249 n. 1 Pt 2, p. H95-101en_US
dc.identifier.issn0002-9513en_US
dc.identifier.urihttp://hdl.handle.net/10722/171310-
dc.description.abstractA bioassay technique was developed to analyze the effect of vasoactive substance(s) released from endothelial cells. Canine femoral arteries with or without endothelium were perfused with physiological salt solution at 37 degrees C. The perfusate was bioassayed with a ring of coronary artery without endothelium. A substance(s) released by the endothelial cells under basal conditions caused relaxation of unstimulated coronary arteries or relaxation of those contracted with prostaglandin F2 alpha. The release of the relaxing substance(s) was augmented by acetylcholine. The relaxation induced by acetylcholine was biphasic: an initial rapid phase followed by a partial recovery and a slowly developing prolonged relaxation; the half-life of the substance(s) causing the initial phase averaged 6.3 s. Norepinephrine, epinephrine, and ascorbic acid, given downstream of the femoral artery, reversibly prevented the second phase but only attenuated the initial relaxation. These observations indicate that an endothelium-derived relaxing substance(s) is released into the lumen of the femoral artery under basal conditions and during stimulation with acetylcholine. Catecholamines can inactivate the relaxing substance(s) but do not prevent either its production by endothelial cells or its action on vascular smooth muscle.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_US
dc.relation.ispartofThe American journal of physiologyen_US
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAscorbic Acid - Pharmacologyen_US
dc.subject.meshCoronary Vessels - Drug Effectsen_US
dc.subject.meshDogsen_US
dc.subject.meshEndothelium - Metabolismen_US
dc.subject.meshEpinephrine - Pharmacologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFemoral Artery - Drug Effectsen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle Contraction - Drug Effectsen_US
dc.subject.meshMuscle, Smooth, Vascular - Drug Effects - Metabolismen_US
dc.subject.meshNitric Oxideen_US
dc.subject.meshNorepinephrine - Pharmacologyen_US
dc.subject.meshPyrazoles - Pharmacologyen_US
dc.subject.meshStimulation, Chemicalen_US
dc.subject.meshVasodilator Agents - Antagonists & Inhibitors - Metabolismen_US
dc.titleBioassay of endothelium-derived relaxing factor(s): inactivation by catecholamines.en_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3874557en_US
dc.identifier.scopuseid_2-s2.0-0347441985en_US
dc.identifier.volume249en_US
dc.identifier.issue1 Pt 2en_US
dc.identifier.spageH95en_US
dc.identifier.epage101en_US
dc.identifier.isiWOS:A1985ALZ0200014-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridRubanyi, GM=7005517991en_US
dc.identifier.scopusauthoridLorenz, RR=7402095192en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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