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Article: K + channels in cultured bovine retinal pericytes: Effects of β-adrenergic stimulation

TitleK + channels in cultured bovine retinal pericytes: Effects of β-adrenergic stimulation
Authors
Issue Date2003
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.cardiovascularpharm.com/
Citation
Journal Of Cardiovascular Pharmacology, 2003, v. 42 n. 3, p. 379-388 How to Cite?
AbstractRetinal pericytes are key cells involved in the regulation of retinal blood flow. The purpose of this work was to identify the K + channel population expressed in cultured bovine retinal pericytes and to determine whether β-adrenergic stimulation alters the activity of these channels. Isolated pericytes were obtained by homogenization and filtration of bovine retina and K + channels were studied with the whole-cell configuration of the patch-clamp technique on 3-5 passaged pericytes. Pericytes expressed an inward current dependent on extracellular K + concentration which was sensitive to micromolar concentrations of barium, a characteristic of an inward-rectifying K + current. Furthermore, two voltage-dependent outward currents were also observed. Their activation and inactivation properties, as well as their respective sensitivity to 4-aminopyridine and iberiotoxin, were indicative of voltage-sensitive and large-conductance calcium-activated K + channels (BK Ca). Isoproterenol and dibutyryl cyclic adenosine monophosphate enhanced the activity of BK Ca without affecting the other potassium currents. In conclusion, bovine retinal pericytes express mainly two outward potassium currents, K v and BK Ca, as well as an inward rectifying K + current, K ir. Physiologic stimuli such as an increase in extracellular potassium concentration or β-adrenergic receptor stimulation enhance the activity of K ir and BK Ca, respectively, suggesting a potential role for these channels in the control of retinal blood flow.
Persistent Identifierhttp://hdl.handle.net/10722/171300
ISSN
2015 Impact Factor: 2.462
2015 SCImago Journal Rankings: 0.962
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQuignard, JFen_US
dc.contributor.authorHarley, EAen_US
dc.contributor.authorDuhault, Jen_US
dc.contributor.authorVanhoutte, PMen_US
dc.contributor.authorFélétou, Men_US
dc.date.accessioned2012-10-30T06:13:16Z-
dc.date.available2012-10-30T06:13:16Z-
dc.date.issued2003en_US
dc.identifier.citationJournal Of Cardiovascular Pharmacology, 2003, v. 42 n. 3, p. 379-388en_US
dc.identifier.issn0160-2446en_US
dc.identifier.urihttp://hdl.handle.net/10722/171300-
dc.description.abstractRetinal pericytes are key cells involved in the regulation of retinal blood flow. The purpose of this work was to identify the K + channel population expressed in cultured bovine retinal pericytes and to determine whether β-adrenergic stimulation alters the activity of these channels. Isolated pericytes were obtained by homogenization and filtration of bovine retina and K + channels were studied with the whole-cell configuration of the patch-clamp technique on 3-5 passaged pericytes. Pericytes expressed an inward current dependent on extracellular K + concentration which was sensitive to micromolar concentrations of barium, a characteristic of an inward-rectifying K + current. Furthermore, two voltage-dependent outward currents were also observed. Their activation and inactivation properties, as well as their respective sensitivity to 4-aminopyridine and iberiotoxin, were indicative of voltage-sensitive and large-conductance calcium-activated K + channels (BK Ca). Isoproterenol and dibutyryl cyclic adenosine monophosphate enhanced the activity of BK Ca without affecting the other potassium currents. In conclusion, bovine retinal pericytes express mainly two outward potassium currents, K v and BK Ca, as well as an inward rectifying K + current, K ir. Physiologic stimuli such as an increase in extracellular potassium concentration or β-adrenergic receptor stimulation enhance the activity of K ir and BK Ca, respectively, suggesting a potential role for these channels in the control of retinal blood flow.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.cardiovascularpharm.com/en_US
dc.relation.ispartofJournal of Cardiovascular Pharmacologyen_US
dc.subject.meshAdrenergic Beta-Agonists - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBarium - Pharmacologyen_US
dc.subject.meshCattleen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCyclic Amp - Physiologyen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshElectric Stimulationen_US
dc.subject.meshElectrophysiologyen_US
dc.subject.meshIsoproterenol - Pharmacologyen_US
dc.subject.meshPatch-Clamp Techniquesen_US
dc.subject.meshPericytes - Drug Effects - Metabolism - Physiologyen_US
dc.subject.meshPotassium Channels - Drug Effectsen_US
dc.subject.meshRetina - Drug Effects - Metabolismen_US
dc.titleK + channels in cultured bovine retinal pericytes: Effects of β-adrenergic stimulationen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/00005344-200309000-00009en_US
dc.identifier.pmid12960683-
dc.identifier.scopuseid_2-s2.0-0041336971en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0041336971&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume42en_US
dc.identifier.issue3en_US
dc.identifier.spage379en_US
dc.identifier.epage388en_US
dc.identifier.isiWOS:000184969800009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridQuignard, JF=13606215800en_US
dc.identifier.scopusauthoridHarley, EA=7005054190en_US
dc.identifier.scopusauthoridDuhault, J=7005108808en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US
dc.identifier.scopusauthoridFélétou, M=7006461826en_US

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