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Article: Expression of the cystic fibrosis transmembrane conductance regulator in rat spermatids: Implication for the site of action of antispermatogenic agents

TitleExpression of the cystic fibrosis transmembrane conductance regulator in rat spermatids: Implication for the site of action of antispermatogenic agents
Authors
Issue Date2001
PublisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/
Citation
Molecular Human Reproduction, 2001, v. 7 n. 8, p. 705-713 How to Cite?
AbstractTo establish whether cystic fibrosis transmembrane conductance regulator (CFTR) is functionally expressed in the testis, we subjected spermatogenic cells from rat testes to analysis of CFTR mRNA, protein and channel activity. CFTR mRNA was detected in the testes of mature but not immature rats using reverse transcription-polymerase chain reaction analysis. Western blot analysis performed with a CFTR specific antibody revealed immunoreactivity in the membrane extract of spermatogenic cells. Immunohistochemical studies localized CFTR in round and elongated spermatids, but not in the fully developed spermatozoa. Using a whole-cell patch clamp technique, we recorded an inward current activated by intracellular cAMP (100 μmol/l) in round spermatids. The current displayed a linear I/V relationship and was inhibited by diphenylamine-2-carboxylate (DPC), a chloride channel blocker. Transfection of the rat germ cell CFTR cDNA into human embryonic kidney (HEK) 293 cells caused the expression of a cAMP-activated chloride current with CFTR characteristics. The current was completely blocked by the antispermatogenic agents 1-(2,4-dichlorobenzyl)-indazole-3-carboxylic acid, lonidamine (500 υmol/l) and 1-(2,4-dichlorobenzyl)-indazole-3-acrylic acid, AF2785 (250 υmol/l). These results taken together provide evidence that CFTR is differentially expressed in spermatids during spermiogenesis. We speculate that CFTR may interact with aquaporin to bring about cytoplasmic volume contraction which is an essential feature of spermiogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/171260
ISSN
2015 Impact Factor: 3.943
2015 SCImago Journal Rankings: 1.611
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGong, XDen_US
dc.contributor.authorLi, JCHen_US
dc.contributor.authorCheung, KHen_US
dc.contributor.authorLeung, GPHen_US
dc.contributor.authorChew, SBCen_US
dc.contributor.authorWong, PYDen_US
dc.date.accessioned2012-10-30T06:13:01Z-
dc.date.available2012-10-30T06:13:01Z-
dc.date.issued2001en_US
dc.identifier.citationMolecular Human Reproduction, 2001, v. 7 n. 8, p. 705-713en_US
dc.identifier.issn1360-9947en_US
dc.identifier.urihttp://hdl.handle.net/10722/171260-
dc.description.abstractTo establish whether cystic fibrosis transmembrane conductance regulator (CFTR) is functionally expressed in the testis, we subjected spermatogenic cells from rat testes to analysis of CFTR mRNA, protein and channel activity. CFTR mRNA was detected in the testes of mature but not immature rats using reverse transcription-polymerase chain reaction analysis. Western blot analysis performed with a CFTR specific antibody revealed immunoreactivity in the membrane extract of spermatogenic cells. Immunohistochemical studies localized CFTR in round and elongated spermatids, but not in the fully developed spermatozoa. Using a whole-cell patch clamp technique, we recorded an inward current activated by intracellular cAMP (100 μmol/l) in round spermatids. The current displayed a linear I/V relationship and was inhibited by diphenylamine-2-carboxylate (DPC), a chloride channel blocker. Transfection of the rat germ cell CFTR cDNA into human embryonic kidney (HEK) 293 cells caused the expression of a cAMP-activated chloride current with CFTR characteristics. The current was completely blocked by the antispermatogenic agents 1-(2,4-dichlorobenzyl)-indazole-3-carboxylic acid, lonidamine (500 υmol/l) and 1-(2,4-dichlorobenzyl)-indazole-3-acrylic acid, AF2785 (250 υmol/l). These results taken together provide evidence that CFTR is differentially expressed in spermatids during spermiogenesis. We speculate that CFTR may interact with aquaporin to bring about cytoplasmic volume contraction which is an essential feature of spermiogenesis.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/en_US
dc.relation.ispartofMolecular Human Reproductionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntispermatogenic Agents - Metabolismen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Separationen_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshCystic Fibrosis Transmembrane Conductance Regulator - Biosynthesis - Genetics - Metabolism - Physiologyen_US
dc.subject.meshDna, Complementary - Geneticsen_US
dc.subject.meshGene Expression Regulation, Developmental - Physiologyen_US
dc.subject.meshGerm Cells - Chemistry - Metabolismen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshMaleen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSeminiferous Tubules - Chemistryen_US
dc.subject.meshSpermatids - Drug Effects - Metabolismen_US
dc.subject.meshSpermatogenesis - Drug Effects - Physiologyen_US
dc.titleExpression of the cystic fibrosis transmembrane conductance regulator in rat spermatids: Implication for the site of action of antispermatogenic agentsen_US
dc.typeArticleen_US
dc.identifier.emailCheung, KH:kingho.cheung@hku.hken_US
dc.identifier.emailLeung, GPH:gphleung@hkucc.hku.hken_US
dc.identifier.authorityCheung, KH=rp01463en_US
dc.identifier.authorityLeung, GPH=rp00234en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/molehr/7.8.705-
dc.identifier.pmid11470857-
dc.identifier.scopuseid_2-s2.0-0034896717en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034896717&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume7en_US
dc.identifier.issue8en_US
dc.identifier.spage705en_US
dc.identifier.epage713en_US
dc.identifier.isiWOS:000170362400003-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridGong, XD=7201999007en_US
dc.identifier.scopusauthoridLi, JCH=7410060637en_US
dc.identifier.scopusauthoridCheung, KH=14007487800en_US
dc.identifier.scopusauthoridLeung, GPH=35963668200en_US
dc.identifier.scopusauthoridChew, SBC=7202524648en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US

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