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Article: Phenotypic and functional changes in regenerated porcine coronary endothelial cells: Increased uptake of modified LDL and reduced production of NO

TitlePhenotypic and functional changes in regenerated porcine coronary endothelial cells: Increased uptake of modified LDL and reduced production of NO
Authors
Issue Date2000
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://circres.ahajournals.org
Citation
Circulation Research, 2000, v. 86 n. 8, p. 854-861 How to Cite?
AbstractPorcine coronary arteries with regenerated endothelium exhibit impaired endothelium-dependent relaxations. Experiments were designed to analyze the structural and functional changes occurring in regenerated endothelial cells. Primary cultures from regenerated endothelium contained giant endothelial cells, with an increased number of cells with diameter > 14.5 μm, a reduced ability to proliferate, and signs of apoptosis. The uptake of fluorescent acetylated LDL was increased 2-fold in cultures from regenerated endothelium. The increased uptake of acetylated LDL was confirmed ex vivo in injured coronary arteries. In cultures from regenerated endothelium, cGMP production was decreased under basal conditions and during stimulation with serotonin, bradykinin, and A23187. Thus, during regeneration, there is accelerated senescence of endothelial cells accompanied by increased incorporation of modified LDL and reduction of NO production without decrease in endothelial NO synthase expression. These alterations help to explain the altered endothelium-dependent responses 28 days after balloon injury.
Persistent Identifierhttp://hdl.handle.net/10722/171253
ISSN
2015 Impact Factor: 11.551
2015 SCImago Journal Rankings: 5.755
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFournetBourguignon, MPen_US
dc.contributor.authorCastedoDelrieu, Men_US
dc.contributor.authorBidouard, JPen_US
dc.contributor.authorLeonce, Sen_US
dc.contributor.authorSaboureau, Den_US
dc.contributor.authorDelescluse, Ien_US
dc.contributor.authorVilaine, JPen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:58Z-
dc.date.available2012-10-30T06:12:58Z-
dc.date.issued2000en_US
dc.identifier.citationCirculation Research, 2000, v. 86 n. 8, p. 854-861en_US
dc.identifier.issn0009-7330en_US
dc.identifier.urihttp://hdl.handle.net/10722/171253-
dc.description.abstractPorcine coronary arteries with regenerated endothelium exhibit impaired endothelium-dependent relaxations. Experiments were designed to analyze the structural and functional changes occurring in regenerated endothelial cells. Primary cultures from regenerated endothelium contained giant endothelial cells, with an increased number of cells with diameter > 14.5 μm, a reduced ability to proliferate, and signs of apoptosis. The uptake of fluorescent acetylated LDL was increased 2-fold in cultures from regenerated endothelium. The increased uptake of acetylated LDL was confirmed ex vivo in injured coronary arteries. In cultures from regenerated endothelium, cGMP production was decreased under basal conditions and during stimulation with serotonin, bradykinin, and A23187. Thus, during regeneration, there is accelerated senescence of endothelial cells accompanied by increased incorporation of modified LDL and reduction of NO production without decrease in endothelial NO synthase expression. These alterations help to explain the altered endothelium-dependent responses 28 days after balloon injury.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://circres.ahajournals.orgen_US
dc.relation.ispartofCirculation Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCoronary Vessels - Metabolism - Physiopathologyen_US
dc.subject.meshEndothelium, Vascular - Metabolism - Physiopathologyen_US
dc.subject.meshLipoproteins, Ldl - Metabolismen_US
dc.subject.meshNitric Oxide - Metabolismen_US
dc.subject.meshNitric Oxide Synthase - Metabolismen_US
dc.subject.meshNitric Oxide Synthase Type Iiien_US
dc.subject.meshRegenerationen_US
dc.subject.meshSwineen_US
dc.titlePhenotypic and functional changes in regenerated porcine coronary endothelial cells: Increased uptake of modified LDL and reduced production of NOen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10785507-
dc.identifier.scopuseid_2-s2.0-0034725070en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034725070&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume86en_US
dc.identifier.issue8en_US
dc.identifier.spage854en_US
dc.identifier.epage861en_US
dc.identifier.isiWOS:000086902800008-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridFournetBourguignon, MP=6507287770en_US
dc.identifier.scopusauthoridCastedoDelrieu, M=6507843556en_US
dc.identifier.scopusauthoridBidouard, JP=6601955808en_US
dc.identifier.scopusauthoridLeonce, S=7005463951en_US
dc.identifier.scopusauthoridSaboureau, D=6602795472en_US
dc.identifier.scopusauthoridDelescluse, I=6506468057en_US
dc.identifier.scopusauthoridVilaine, JP=7004617134en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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