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Article: Polarized distribution of NHE1 and NHE2 in the rat epididymis
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TitlePolarized distribution of NHE1 and NHE2 in the rat epididymis
 
AuthorsCheng Chew, SB2
Leung, GPH2
Leung, PY2
Tse, CM1
Wong, PYD2
 
Issue Date2000
 
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
 
CitationBiology Of Reproduction, 2000, v. 62 n. 3, p. 755-758 [How to Cite?]
DOI: http://dx.doi.org/10.1095/biolreprod62.3.755
 
AbstractPrevious studies from our laboratory have provided evidence that the rat epididymis utilizes the Na+/H+ exchanger to transport acid and base. The present study was undertaken to use immunohistochemistry for investigating the localization (apical versus basolateral) and distribution of NHE1 and NHE2 proteins along intact rat epididymis. Both proteins were found to be exclusively localized within the epithelium. Immunoreactivity for NHE1 was detected on the basolateral surface, whereas NHE2 immunoreactivity was detected on the apical side of the epithelium. Interestingly, NHE1 was found along the entire length of the epididymal tubule whereas NHE2 was absent in the initial segment but present in the caput, corpus, and cauda regions. These results, when interpreted along with those of previous functional studies, may suggest that the apical NHE2 is involved in Na+ reabsorption and the basolateral NHE1 in HCO3 - secretion in the rat epididymis.
 
ISSN0006-3363
2013 Impact Factor: 3.451
 
DOIhttp://dx.doi.org/10.1095/biolreprod62.3.755
 
ISI Accession Number IDWOS:000085533500034
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorCheng Chew, SB
 
dc.contributor.authorLeung, GPH
 
dc.contributor.authorLeung, PY
 
dc.contributor.authorTse, CM
 
dc.contributor.authorWong, PYD
 
dc.date.accessioned2012-10-30T06:12:53Z
 
dc.date.available2012-10-30T06:12:53Z
 
dc.date.issued2000
 
dc.description.abstractPrevious studies from our laboratory have provided evidence that the rat epididymis utilizes the Na+/H+ exchanger to transport acid and base. The present study was undertaken to use immunohistochemistry for investigating the localization (apical versus basolateral) and distribution of NHE1 and NHE2 proteins along intact rat epididymis. Both proteins were found to be exclusively localized within the epithelium. Immunoreactivity for NHE1 was detected on the basolateral surface, whereas NHE2 immunoreactivity was detected on the apical side of the epithelium. Interestingly, NHE1 was found along the entire length of the epididymal tubule whereas NHE2 was absent in the initial segment but present in the caput, corpus, and cauda regions. These results, when interpreted along with those of previous functional studies, may suggest that the apical NHE2 is involved in Na+ reabsorption and the basolateral NHE1 in HCO3 - secretion in the rat epididymis.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationBiology Of Reproduction, 2000, v. 62 n. 3, p. 755-758 [How to Cite?]
DOI: http://dx.doi.org/10.1095/biolreprod62.3.755
 
dc.identifier.doihttp://dx.doi.org/10.1095/biolreprod62.3.755
 
dc.identifier.epage758
 
dc.identifier.isiWOS:000085533500034
 
dc.identifier.issn0006-3363
2013 Impact Factor: 3.451
 
dc.identifier.issue3
 
dc.identifier.pmid10684820
 
dc.identifier.scopuseid_2-s2.0-0033996367
 
dc.identifier.spage755
 
dc.identifier.urihttp://hdl.handle.net/10722/171238
 
dc.identifier.volume62
 
dc.languageeng
 
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofBiology of Reproduction
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshCell Membrane - Metabolism
 
dc.subject.meshEpididymis - Metabolism
 
dc.subject.meshImmunoenzyme Techniques
 
dc.subject.meshMale
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshSodium-Hydrogen Antiporter - Immunology - Metabolism
 
dc.titlePolarized distribution of NHE1 and NHE2 in the rat epididymis
 
dc.typeArticle
 
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<contributor.author>Leung, GPH</contributor.author>
<contributor.author>Leung, PY</contributor.author>
<contributor.author>Tse, CM</contributor.author>
<contributor.author>Wong, PYD</contributor.author>
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<description.abstract>Previous studies from our laboratory have provided evidence that the rat epididymis utilizes the Na+/H+ exchanger to transport acid and base. The present study was undertaken to use immunohistochemistry for investigating the localization (apical versus basolateral) and distribution of NHE1 and NHE2 proteins along intact rat epididymis. Both proteins were found to be exclusively localized within the epithelium. Immunoreactivity for NHE1 was detected on the basolateral surface, whereas NHE2 immunoreactivity was detected on the apical side of the epithelium. Interestingly, NHE1 was found along the entire length of the epididymal tubule whereas NHE2 was absent in the initial segment but present in the caput, corpus, and cauda regions. These results, when interpreted along with those of previous functional studies, may suggest that the apical NHE2 is involved in Na+ reabsorption and the basolateral NHE1 in HCO3 - secretion in the rat epididymis.</description.abstract>
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Author Affiliations
  1. Johns Hopkins University
  2. Chinese University of Hong Kong