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Article: Prostaglandins but not nitric oxide are endothelium-derived relaxing factors in the trout aorta

TitleProstaglandins but not nitric oxide are endothelium-derived relaxing factors in the trout aorta
Authors
Issue Date2000
Citation
Acta Pharmacologica Sinica, 2000, v. 21 n. 10, p. 871-876 How to Cite?
AbstractAIM: To identify the type of prostanoids produced by endothelial cells of trout aorta and to determine whether or not the smooth muscle responds to nitric oxide. METHODS: Ventral aortas, with and without endothelium from rainbow trout (S gairdneri), were incubated in a buffered salt solution. RESULTS: Addition of the calcium ionophore A23187 caused a significant increase in prostaglandin E's and a consistent increase in the stable metabolite of prostacyclin (6-keto-prostaglandin F(1α)) in the incubation media only when the endothelium was present. This production was inhibited by methylene blue (10 μmol/L). In rings of trout aorta without endothelium suspended for the measurement of isometric force in organ chambers, prostacyclin and prostaglandin E1 but not prostaglandin E2 caused concentration-dependent decreases in tension when the rings were contracted with acetylcholine. The smooth muscle did not relax to nitric oxide but did so to sodium nitroprusside. Relaxations to the latter nitrovasodilator were not inhibited by methylene blue. Descending aorta without endothelium from frogs relaxed in a concentration-dependent manner to nitric oxide. CONCLUSION: Predominant endothelium-derived relaxing factors in trout aorta are prostaglandins, the synthesis of which can be inhibited by methylene blue. A phylogenetic appearance of nitric-oxide sensitive mechanism for vasodilatation, perhaps is associated with the transition from water to air respiration.
Persistent Identifierhttp://hdl.handle.net/10722/171232
ISSN
2000 Impact Factor: 0.485
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMiller, VMen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:50Z-
dc.date.available2012-10-30T06:12:50Z-
dc.date.issued2000en_US
dc.identifier.citationActa Pharmacologica Sinica, 2000, v. 21 n. 10, p. 871-876en_US
dc.identifier.issn0253-9756en_US
dc.identifier.urihttp://hdl.handle.net/10722/171232-
dc.description.abstractAIM: To identify the type of prostanoids produced by endothelial cells of trout aorta and to determine whether or not the smooth muscle responds to nitric oxide. METHODS: Ventral aortas, with and without endothelium from rainbow trout (S gairdneri), were incubated in a buffered salt solution. RESULTS: Addition of the calcium ionophore A23187 caused a significant increase in prostaglandin E's and a consistent increase in the stable metabolite of prostacyclin (6-keto-prostaglandin F(1α)) in the incubation media only when the endothelium was present. This production was inhibited by methylene blue (10 μmol/L). In rings of trout aorta without endothelium suspended for the measurement of isometric force in organ chambers, prostacyclin and prostaglandin E1 but not prostaglandin E2 caused concentration-dependent decreases in tension when the rings were contracted with acetylcholine. The smooth muscle did not relax to nitric oxide but did so to sodium nitroprusside. Relaxations to the latter nitrovasodilator were not inhibited by methylene blue. Descending aorta without endothelium from frogs relaxed in a concentration-dependent manner to nitric oxide. CONCLUSION: Predominant endothelium-derived relaxing factors in trout aorta are prostaglandins, the synthesis of which can be inhibited by methylene blue. A phylogenetic appearance of nitric-oxide sensitive mechanism for vasodilatation, perhaps is associated with the transition from water to air respiration.en_US
dc.languageengen_US
dc.relation.ispartofActa Pharmacologica Sinicaen_US
dc.subject.mesh6-Ketoprostaglandin F1 Alpha - Biosynthesisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAortaen_US
dc.subject.meshCalcimycin - Pharmacologyen_US
dc.subject.meshEndothelium, Vascular - Metabolismen_US
dc.subject.meshMuscle, Smooth, Vascular - Drug Effectsen_US
dc.subject.meshNitric Oxide - Pharmacologyen_US
dc.subject.meshProstaglandins E - Biosynthesisen_US
dc.subject.meshRana Catesbeianaen_US
dc.subject.meshTrouten_US
dc.subject.meshVasodilation - Drug Effectsen_US
dc.titleProstaglandins but not nitric oxide are endothelium-derived relaxing factors in the trout aortaen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11501036-
dc.identifier.scopuseid_2-s2.0-0033774865en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033774865&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume21en_US
dc.identifier.issue10en_US
dc.identifier.spage871en_US
dc.identifier.epage876en_US
dc.identifier.isiWOS:000089725800002-
dc.identifier.scopusauthoridMiller, VM=7201476816en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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