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Article: Serotonin via 5-HT(1B) and 5-HT(2B) receptors stimulates anion secretion in the rat epididymal epithelium

TitleSerotonin via 5-HT(1B) and 5-HT(2B) receptors stimulates anion secretion in the rat epididymal epithelium
Authors
Issue Date1999
PublisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3751
Citation
Journal Of Physiology, 1999, v. 519 n. 3, p. 657-667 How to Cite?
Abstract1. The short-circuit current (I(sc)) technique was used to study the role of 5-hydroxytryptamine (5-HT) in the regulation of anion secretion in cultured rat cauda epididymal epithelia. 2. 5-HT, the 5-HT(1B)-selective agonist 5-nonyloxytryptamine (5-NOT) and the 5-HT(2B)-selective agonist α-methyl-5-hydroxytryptamine (α-methyl-5-HT) added basolaterally stimulated I(sc) in a dose-dependent manner with EC50 values of 0.4, 20 and 0.3 μM, respectively. No other agonists for 5-HT receptors had any effect. 3. The pattern of responses to 5-HT was biphasic. Pretreating the tissues with 5-HT(1B)-selective antagonist isamoltane (200 μM) and the 5-HT(2B)-selective antagonist rauwolscine (200 μM) inhibited the rapid transient phase by 55 and 45%, whereas the sustained phase could only be blocked by rauwolscine. 4. Removal of chloride or bicarbonate or both from the normal Krebs-Henseleit solution reduced the response to 5-HT, 5-NOT and α-methyl-5-HT to varying degrees. The results suggest that 5-HT(1B)- and 5-HT(2B)-mediated responses were mainly due to chloride and bicarbonate secretion, respectively. 5. Manipulation of the cAMP and Ca2+ signal transduction pathways with chemical agents provided evidence that the responses to 5-HT were mediated through cAMP. Piroxicam pretreatment abolished the I(sc) response to α-methyl-5-HT but not to 5-NOT, indicating that the 5-HT(2B)-mediated response, but not the 5-HT(1B)-mediated response, is dependent on prostaglandin synthesis. 7. Immunohistochemical studies showed that 5-HT-like immunoreactivity was detected in nerve fibres and in small granular cells surrounding the epididymal tubules. 8. It is suggested that the 5-HT released from serotonergic nerve endings and/or from mast cells regulates electrolyte and fluid secretion in the epididymis.
Persistent Identifierhttp://hdl.handle.net/10722/171230
ISSN
2015 Impact Factor: 4.731
2015 SCImago Journal Rankings: 2.670
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, GPHen_US
dc.contributor.authorDun, SLen_US
dc.contributor.authorDun, NJen_US
dc.contributor.authorWong, PYDen_US
dc.date.accessioned2012-10-30T06:12:49Z-
dc.date.available2012-10-30T06:12:49Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of Physiology, 1999, v. 519 n. 3, p. 657-667en_US
dc.identifier.issn0022-3751en_US
dc.identifier.urihttp://hdl.handle.net/10722/171230-
dc.description.abstract1. The short-circuit current (I(sc)) technique was used to study the role of 5-hydroxytryptamine (5-HT) in the regulation of anion secretion in cultured rat cauda epididymal epithelia. 2. 5-HT, the 5-HT(1B)-selective agonist 5-nonyloxytryptamine (5-NOT) and the 5-HT(2B)-selective agonist α-methyl-5-hydroxytryptamine (α-methyl-5-HT) added basolaterally stimulated I(sc) in a dose-dependent manner with EC50 values of 0.4, 20 and 0.3 μM, respectively. No other agonists for 5-HT receptors had any effect. 3. The pattern of responses to 5-HT was biphasic. Pretreating the tissues with 5-HT(1B)-selective antagonist isamoltane (200 μM) and the 5-HT(2B)-selective antagonist rauwolscine (200 μM) inhibited the rapid transient phase by 55 and 45%, whereas the sustained phase could only be blocked by rauwolscine. 4. Removal of chloride or bicarbonate or both from the normal Krebs-Henseleit solution reduced the response to 5-HT, 5-NOT and α-methyl-5-HT to varying degrees. The results suggest that 5-HT(1B)- and 5-HT(2B)-mediated responses were mainly due to chloride and bicarbonate secretion, respectively. 5. Manipulation of the cAMP and Ca2+ signal transduction pathways with chemical agents provided evidence that the responses to 5-HT were mediated through cAMP. Piroxicam pretreatment abolished the I(sc) response to α-methyl-5-HT but not to 5-NOT, indicating that the 5-HT(2B)-mediated response, but not the 5-HT(1B)-mediated response, is dependent on prostaglandin synthesis. 7. Immunohistochemical studies showed that 5-HT-like immunoreactivity was detected in nerve fibres and in small granular cells surrounding the epididymal tubules. 8. It is suggested that the 5-HT released from serotonergic nerve endings and/or from mast cells regulates electrolyte and fluid secretion in the epididymis.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3751en_US
dc.relation.ispartofJournal of Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnions - Metabolismen_US
dc.subject.meshChelating Agents - Pharmacologyen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshEgtazic Acid - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshEnzyme Inhibitors - Pharmacologyen_US
dc.subject.meshEpididymis - Secretionen_US
dc.subject.meshEpithelium - Metabolismen_US
dc.subject.meshForskolin - Pharmacologyen_US
dc.subject.meshImines - Pharmacologyen_US
dc.subject.meshIsoquinolines - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptor, Serotonin, 5-Ht1ben_US
dc.subject.meshReceptor, Serotonin, 5-Ht2ben_US
dc.subject.meshReceptors, Serotonin - Drug Effects - Metabolismen_US
dc.subject.meshSerotonin - Metabolismen_US
dc.subject.meshSulfonamidesen_US
dc.subject.meshThapsigargin - Pharmacologyen_US
dc.titleSerotonin via 5-HT(1B) and 5-HT(2B) receptors stimulates anion secretion in the rat epididymal epitheliumen_US
dc.typeArticleen_US
dc.identifier.emailLeung, GPH:gphleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, GPH=rp00234en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1469-7793.1999.0657n.xen_US
dc.identifier.pmid10457081-
dc.identifier.scopuseid_2-s2.0-0033199936en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033199936&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume519en_US
dc.identifier.issue3en_US
dc.identifier.spage657en_US
dc.identifier.epage667en_US
dc.identifier.isiWOS:000083031500004-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLeung, GPH=35963668200en_US
dc.identifier.scopusauthoridDun, SL=7003778066en_US
dc.identifier.scopusauthoridDun, NJ=7004670725en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US

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