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Article: Hyperpolarization caused by serotonin contributes to endothelium- dependent relaxations in the porcine coronary artery

TitleHyperpolarization caused by serotonin contributes to endothelium- dependent relaxations in the porcine coronary artery
Authors
Issue Date1999
Citation
Acta Pharmacologica Sinica, 1999, v. 20 n. 12, p. 1093-1097 How to Cite?
AbstractAIM: The present study was designed to investigate the contribution of membrane hyperpolarization to endothelium-dependent relaxations induced by serotonin in the porcine coronary artery. METHODS: Rings with and without endothelium of porcine coronary arteries were suspended in conventional organ chambers for the measurement of isometric force. The cell membrane potential of the vascular smooth muscle cells was measured using glass microelectrodes, in the presence of indometacin, ketanserin, and/or N(ω)-nitro-L-arginine. RESULTS: Serotonin induced a transient endothelium-, and concentration- dependent relaxation in rings contracted with prostaglandin F(2α) in the presence of N(ω)-nitro-L-arginine (maximal relaxation: 19%). The N(ω)- nitro-L-arginine resistant relaxation was abolished by high K+ and tetrabutylammonium chloride. Serotonin also caused an endothelium-, concentration-dependent membrane hyperpolarizations with a maximal amplitude of -8.8mV. The nitro-L-arginine resistant relaxations and hyperpolarizations were abolished by methiothepin, but not by glibenclamide. The time course of the endothelium-dependent relaxations and hyperpolarizations was similar. CONCLUSION: These results suggest a contribution of cell membrane hyperpolarization to the endothelium-dependent relaxations induced by serotonin in the porcine coronary artery.
Persistent Identifierhttp://hdl.handle.net/10722/171220
ISSN
2000 Impact Factor: 0.485
References

 

DC FieldValueLanguage
dc.contributor.authorPark, SJen_US
dc.contributor.authorNakashima, Men_US
dc.contributor.authorNagao, Ten_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:47Z-
dc.date.available2012-10-30T06:12:47Z-
dc.date.issued1999en_US
dc.identifier.citationActa Pharmacologica Sinica, 1999, v. 20 n. 12, p. 1093-1097en_US
dc.identifier.issn0253-9756en_US
dc.identifier.urihttp://hdl.handle.net/10722/171220-
dc.description.abstractAIM: The present study was designed to investigate the contribution of membrane hyperpolarization to endothelium-dependent relaxations induced by serotonin in the porcine coronary artery. METHODS: Rings with and without endothelium of porcine coronary arteries were suspended in conventional organ chambers for the measurement of isometric force. The cell membrane potential of the vascular smooth muscle cells was measured using glass microelectrodes, in the presence of indometacin, ketanserin, and/or N(ω)-nitro-L-arginine. RESULTS: Serotonin induced a transient endothelium-, and concentration- dependent relaxation in rings contracted with prostaglandin F(2α) in the presence of N(ω)-nitro-L-arginine (maximal relaxation: 19%). The N(ω)- nitro-L-arginine resistant relaxation was abolished by high K+ and tetrabutylammonium chloride. Serotonin also caused an endothelium-, concentration-dependent membrane hyperpolarizations with a maximal amplitude of -8.8mV. The nitro-L-arginine resistant relaxations and hyperpolarizations were abolished by methiothepin, but not by glibenclamide. The time course of the endothelium-dependent relaxations and hyperpolarizations was similar. CONCLUSION: These results suggest a contribution of cell membrane hyperpolarization to the endothelium-dependent relaxations induced by serotonin in the porcine coronary artery.en_US
dc.languageengen_US
dc.relation.ispartofActa Pharmacologica Sinicaen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCoronary Vessels - Drug Effectsen_US
dc.subject.meshEndothelium, Vascular - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Potentials - Drug Effectsen_US
dc.subject.meshMethiothepin - Pharmacologyen_US
dc.subject.meshMuscle Relaxation - Drug Effectsen_US
dc.subject.meshMuscle, Smooth, Vascular - Drug Effectsen_US
dc.subject.meshPotassium Channel Blockersen_US
dc.subject.meshQuaternary Ammonium Compounds - Pharmacologyen_US
dc.subject.meshSerotonin - Pharmacologyen_US
dc.subject.meshSerotonin Antagonists - Pharmacologyen_US
dc.subject.meshSwineen_US
dc.titleHyperpolarization caused by serotonin contributes to endothelium- dependent relaxations in the porcine coronary arteryen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11189198-
dc.identifier.scopuseid_2-s2.0-0032761991en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032761991&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume20en_US
dc.identifier.issue12en_US
dc.identifier.spage1093en_US
dc.identifier.epage1097en_US
dc.identifier.scopusauthoridPark, SJ=7501828826en_US
dc.identifier.scopusauthoridNakashima, M=35599797500en_US
dc.identifier.scopusauthoridNagao, T=7401489430en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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