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Article: Abnormal deposition of extracellular matrix proteins by cultured smooth muscle cells from human varicose veins

TitleAbnormal deposition of extracellular matrix proteins by cultured smooth muscle cells from human varicose veins
Authors
Issue Date1998
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVR
Citation
Journal Of Vascular Research, 1998, v. 35 n. 2, p. 115-123 How to Cite?
AbstractThe aim of the present study was to verify whether the modifications of the extracellular matrix, described in varicose veins, are also present in cultures of smooth muscle cells from human varicose veins. The accumulation of collagen type Iii and fibronectin was determined by immunofluorescence in cultures of smooth muscle cells at passage 2-3 during the proliferation phase. After 5 days of culture, the immunostaining of both collagen type Iii and fibronectin was weaker in cells from varicose than in those of control veins while the expression of collagen type Iii and fibronectin messenger ribonucleic acids was not significantly different. Collagen type I and Iii synthesis were quantified by tritiated proline incorporation in control and varicose cell layers at postconfluence. Collagen type I deposition was similar in both types of cell layers while collagen type Iii was decreased in cell layers from varicose veins. Matrix metalloproteinases (mmps) and their inhibitors (timps) were also quantified by enzyme immunoassays in supernatants from smooth muscle cell cultures at postconfluence. No significant difference was observed in the synthesis of any of the Mmps (- 1, -2 and -9) or their inhibitors (- 1 and -2) tested. These data illustrate that smooth muscle cells cultured from varicose veins deposit less collagen type Iii and fibronectin than control cells despite comparable levels of mrnas for these proteins suggesting dysregulation of posttranslational steps in the synthesis of both proteins by smooth muscle cells from varicose veins.
Persistent Identifierhttp://hdl.handle.net/10722/171210
ISSN
2015 Impact Factor: 2.186
2015 SCImago Journal Rankings: 1.119
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSansilvestriMorel, Pen_US
dc.contributor.authorNonotte, Ien_US
dc.contributor.authorFournetBourguignon, MPen_US
dc.contributor.authorRupìn, Aen_US
dc.contributor.authorFabiani, JNen_US
dc.contributor.authorVerbeuren, TJen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:43Z-
dc.date.available2012-10-30T06:12:43Z-
dc.date.issued1998en_US
dc.identifier.citationJournal Of Vascular Research, 1998, v. 35 n. 2, p. 115-123en_US
dc.identifier.issn1018-1172en_US
dc.identifier.urihttp://hdl.handle.net/10722/171210-
dc.description.abstractThe aim of the present study was to verify whether the modifications of the extracellular matrix, described in varicose veins, are also present in cultures of smooth muscle cells from human varicose veins. The accumulation of collagen type Iii and fibronectin was determined by immunofluorescence in cultures of smooth muscle cells at passage 2-3 during the proliferation phase. After 5 days of culture, the immunostaining of both collagen type Iii and fibronectin was weaker in cells from varicose than in those of control veins while the expression of collagen type Iii and fibronectin messenger ribonucleic acids was not significantly different. Collagen type I and Iii synthesis were quantified by tritiated proline incorporation in control and varicose cell layers at postconfluence. Collagen type I deposition was similar in both types of cell layers while collagen type Iii was decreased in cell layers from varicose veins. Matrix metalloproteinases (mmps) and their inhibitors (timps) were also quantified by enzyme immunoassays in supernatants from smooth muscle cell cultures at postconfluence. No significant difference was observed in the synthesis of any of the Mmps (- 1, -2 and -9) or their inhibitors (- 1 and -2) tested. These data illustrate that smooth muscle cells cultured from varicose veins deposit less collagen type Iii and fibronectin than control cells despite comparable levels of mrnas for these proteins suggesting dysregulation of posttranslational steps in the synthesis of both proteins by smooth muscle cells from varicose veins.en_US
dc.languageengen_US
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVRen_US
dc.relation.ispartofJournal of Vascular Researchen_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshCell Division - Physiologyen_US
dc.subject.meshCell Movement - Physiologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCollagen - Metabolismen_US
dc.subject.meshExtracellular Matrix Proteins - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshFibronectins - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMetalloendopeptidases - Metabolismen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMuscle, Smooth, Vascular - Metabolism - Pathologyen_US
dc.subject.meshReference Valuesen_US
dc.subject.meshTissue Inhibitor Of Metalloproteinases - Metabolismen_US
dc.subject.meshVaricose Veins - Metabolism - Pathologyen_US
dc.titleAbnormal deposition of extracellular matrix proteins by cultured smooth muscle cells from human varicose veinsen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1159/000025573en_US
dc.identifier.pmid9588875-
dc.identifier.scopuseid_2-s2.0-0031896488en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031896488&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume35en_US
dc.identifier.issue2en_US
dc.identifier.spage115en_US
dc.identifier.epage123en_US
dc.identifier.isiWOS:000073325900006-
dc.publisher.placeSwitzerlanden_US
dc.identifier.scopusauthoridSansilvestriMorel, P=7801596301en_US
dc.identifier.scopusauthoridNonotte, I=6507010811en_US
dc.identifier.scopusauthoridFournetBourguignon, MP=6507287770en_US
dc.identifier.scopusauthoridRupìn, A=6701753601en_US
dc.identifier.scopusauthoridFabiani, JN=7102990953en_US
dc.identifier.scopusauthoridVerbeuren, TJ=7007006534en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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