File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Regulation of nitric oxide-like activity by prostanoids in smooth muscle of the canine saphenous vein

TitleRegulation of nitric oxide-like activity by prostanoids in smooth muscle of the canine saphenous vein
Authors
Issue Date1996
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 1996, v. 117 n. 2, p. 360-364 How to Cite?
Abstract1 Organ bath experiments and measurements of prostanoids were performed to investigate the presence of nitric oxide synthase in venous smooth muscle and its interaction with cyclo-oxygenase. 2 In rings of canine saphenous vein without endothelium, the inhibitor of cyclo-oxygenase, indomethacin (10 μM), induced contraction. N(G)-nitro-L-arginine (100 μM) (L-NOARG), an inhibitor of nitric oxide synthase did not affect the tone of rings of canine saphenous vein when administered alone. However, in the presence of indomethacin L-NOARG (100 μM) induced further contraction. 3 Similar results were obtained in response to N(G)-monomethyl-L-arginine (L-NMMA)(300 μM or N(G)-nitro-L-arginine methylester (L-NAME)(100 μM). 4 When rings of canine saphenous vein without endothelium were contracted with phenylephrine (1 μM) instead of indomethacin, neither L-NOARG or L-NMMA induced further contraction. 5 When rings of canine saphenous vein without endothelium were contracted with noradrenaline (0.3 μM) in the presence of indomethacin (10 μM) plus L-NOARG (100 μM), a relaxation to L-arginine was observed. Transient relaxations to superoxide dismutase (150 u ml-1) were observed in all rings. 6 When rings of saphenous vein without endothelium were incubated with lipopolysaccharide (LPS) (100 μg ml-1) or interleukin -1β (10 u ml-1) the concentration-contraction curve to noradrenaline was not affected. 7 Rings without endothelium released prostaglandin E2 and prostaglandin I2, as measured by radiommunoassay. The basal production was abolished by indomethacin and not affected by L-NOARG. 8 These results suggest that when cyclo-oxygenase is inhibited, a nitric oxide synthase activity is revealed in rings of canine saphenous vein without endothelium.
Persistent Identifierhttp://hdl.handle.net/10722/171188
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorIlliano, Sen_US
dc.contributor.authorMarsault, Ren_US
dc.contributor.authorDescombes, JJen_US
dc.contributor.authorVerbeuren, Ten_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:35Z-
dc.date.available2012-10-30T06:12:35Z-
dc.date.issued1996en_US
dc.identifier.citationBritish Journal Of Pharmacology, 1996, v. 117 n. 2, p. 360-364en_US
dc.identifier.issn0007-1188en_US
dc.identifier.urihttp://hdl.handle.net/10722/171188-
dc.description.abstract1 Organ bath experiments and measurements of prostanoids were performed to investigate the presence of nitric oxide synthase in venous smooth muscle and its interaction with cyclo-oxygenase. 2 In rings of canine saphenous vein without endothelium, the inhibitor of cyclo-oxygenase, indomethacin (10 μM), induced contraction. N(G)-nitro-L-arginine (100 μM) (L-NOARG), an inhibitor of nitric oxide synthase did not affect the tone of rings of canine saphenous vein when administered alone. However, in the presence of indomethacin L-NOARG (100 μM) induced further contraction. 3 Similar results were obtained in response to N(G)-monomethyl-L-arginine (L-NMMA)(300 μM or N(G)-nitro-L-arginine methylester (L-NAME)(100 μM). 4 When rings of canine saphenous vein without endothelium were contracted with phenylephrine (1 μM) instead of indomethacin, neither L-NOARG or L-NMMA induced further contraction. 5 When rings of canine saphenous vein without endothelium were contracted with noradrenaline (0.3 μM) in the presence of indomethacin (10 μM) plus L-NOARG (100 μM), a relaxation to L-arginine was observed. Transient relaxations to superoxide dismutase (150 u ml-1) were observed in all rings. 6 When rings of saphenous vein without endothelium were incubated with lipopolysaccharide (LPS) (100 μg ml-1) or interleukin -1β (10 u ml-1) the concentration-contraction curve to noradrenaline was not affected. 7 Rings without endothelium released prostaglandin E2 and prostaglandin I2, as measured by radiommunoassay. The basal production was abolished by indomethacin and not affected by L-NOARG. 8 These results suggest that when cyclo-oxygenase is inhibited, a nitric oxide synthase activity is revealed in rings of canine saphenous vein without endothelium.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_US
dc.relation.ispartofBritish Journal of Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCyclooxygenase Inhibitors - Pharmacologyen_US
dc.subject.meshDinoprostone - Pharmacologyen_US
dc.subject.meshDogsen_US
dc.subject.meshEndothelium, Vascular - Drug Effects - Enzymologyen_US
dc.subject.meshEnzyme Inhibitors - Pharmacologyen_US
dc.subject.meshEpoprostenol - Pharmacologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshIndomethacin - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle, Smooth, Vascular - Enzymology - Physiologyen_US
dc.subject.meshNg-Nitroarginine Methyl Ester - Pharmacologyen_US
dc.subject.meshNitric Oxide - Physiologyen_US
dc.subject.meshNitric Oxide Synthase - Antagonists & Inhibitors - Physiologyen_US
dc.subject.meshNitroarginine - Pharmacologyen_US
dc.subject.meshNorepinephrine - Pharmacologyen_US
dc.subject.meshProstaglandins - Physiologyen_US
dc.subject.meshSaphenous Vein - Enzymology - Physiologyen_US
dc.subject.meshVasoconstrictor Agents - Pharmacologyen_US
dc.titleRegulation of nitric oxide-like activity by prostanoids in smooth muscle of the canine saphenous veinen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1476-5381.1996.tb15199.x-
dc.identifier.pmid8789391-
dc.identifier.scopuseid_2-s2.0-0030065535en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030065535&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume117en_US
dc.identifier.issue2en_US
dc.identifier.spage360en_US
dc.identifier.epage364en_US
dc.identifier.isiWOS:A1996TY67500020-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridIlliano, S=6602119848en_US
dc.identifier.scopusauthoridMarsault, R=6603857574en_US
dc.identifier.scopusauthoridDescombes, JJ=6602832507en_US
dc.identifier.scopusauthoridVerbeuren, T=7007006534en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats