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Article: Bioassay of endothelium-derived hyperpolarizing factor

TitleBioassay of endothelium-derived hyperpolarizing factor
Authors
Issue Date1996
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 1996, v. 221 n. 2, p. 484-488 How to Cite?
AbstractEndothelium-derived hyperpolarizing factor (EDHF) mediates vasodilatation in certain blood vessels, together with prostacyclin and NO. However, its chemical nature is not known. A perfusion-superfusion cascade was developed to confirm the diffusible nature of EDHF. Canine carotid arteries with endothelium were used as donors of vasoactive substances, whereas rings of coronary artery without endothelium were used as detectors. Inhibitors of NO synthesis and cyclooxygenase were present throughout, to avoid interference from NO and prostanoids. Measurements of membrane potential and isometric tension, in coronary arteries without endothelium (used as detectors), demonstrated the release of EDHF from the carotid arteries, following treatment with 8-methoxypsoralen, bradykinin and thimerosal. The K+-channel blocker tetraethylammonium inhibited the action of EDHF in the detectors. Thus, these results demonstrate that endothelial cells release a diffusible activator of K+-channels in vascular smooth muscle.
Persistent Identifierhttp://hdl.handle.net/10722/171186
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMombouli, JVen_US
dc.contributor.authorBissiriou, Ien_US
dc.contributor.authorAgboton, VDen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:35Z-
dc.date.available2012-10-30T06:12:35Z-
dc.date.issued1996en_US
dc.identifier.citationBiochemical And Biophysical Research Communications, 1996, v. 221 n. 2, p. 484-488en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/171186-
dc.description.abstractEndothelium-derived hyperpolarizing factor (EDHF) mediates vasodilatation in certain blood vessels, together with prostacyclin and NO. However, its chemical nature is not known. A perfusion-superfusion cascade was developed to confirm the diffusible nature of EDHF. Canine carotid arteries with endothelium were used as donors of vasoactive substances, whereas rings of coronary artery without endothelium were used as detectors. Inhibitors of NO synthesis and cyclooxygenase were present throughout, to avoid interference from NO and prostanoids. Measurements of membrane potential and isometric tension, in coronary arteries without endothelium (used as detectors), demonstrated the release of EDHF from the carotid arteries, following treatment with 8-methoxypsoralen, bradykinin and thimerosal. The K+-channel blocker tetraethylammonium inhibited the action of EDHF in the detectors. Thus, these results demonstrate that endothelial cells release a diffusible activator of K+-channels in vascular smooth muscle.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Assayen_US
dc.subject.meshBiological Factors - Metabolismen_US
dc.subject.meshBradykinin - Pharmacologyen_US
dc.subject.meshCarotid Arteries - Drug Effects - Metabolism - Physiologyen_US
dc.subject.meshCoronary Vessels - Drug Effects - Metabolism - Physiologyen_US
dc.subject.meshDogsen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Potentialsen_US
dc.subject.meshMethoxsalen - Pharmacologyen_US
dc.subject.meshMuscle Relaxationen_US
dc.subject.meshThimerosal - Pharmacologyen_US
dc.titleBioassay of endothelium-derived hyperpolarizing factoren_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/bbrc.1996.0621en_US
dc.identifier.pmid8619881-
dc.identifier.scopuseid_2-s2.0-0030008229en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030008229&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume221en_US
dc.identifier.issue2en_US
dc.identifier.spage484en_US
dc.identifier.epage488en_US
dc.identifier.isiWOS:A1996UF91100047-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMombouli, JV=7004285772en_US
dc.identifier.scopusauthoridBissiriou, I=8052855800en_US
dc.identifier.scopusauthoridAgboton, VD=8052855900en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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