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Article: Thrombin prevents the expression of inducible nitric oxide synthase in vascular smooth muscle cells by a proteolytically-activated thrombin receptor

TitleThrombin prevents the expression of inducible nitric oxide synthase in vascular smooth muscle cells by a proteolytically-activated thrombin receptor
Authors
Issue Date1995
PublisherSchattauer GmbH. The Journal's web site is located at http://www.thrombosis-online.com
Citation
Thrombosis And Haemostasis, 1995, v. 74 n. 3, p. 980-986 How to Cite?
AbstractProteolytically active forms of thrombin (α- and γ-thrombin) and thrombin receptor peptides inhibited the release of nitrite, a stable endproduct of nitric oxide, evoked by interleukin-1 β (IL-1 β) in cultured vascular smooth muscle cells while proteolytically inactive forms [D-Phe-Pro-Arg chloromethyl ketone-α-thrombin (PPACK-α-thrombin) and diisopropylphosphoryl-α-thrombin (DIP-α-thrombin)] had either no or only minimal inhibitory effects. Under bioassay conditions, perfusates from columns containing IL-1 β-activated vascular smooth muscle cells or cells treated with IL-1 β plus PPACK-α-thrombin relaxed detector blood vessels. These relaxations were abolished by the inhibitor of nitric oxide synthesis, N(G)-nitro-L-arginine. No relaxations were obtained with untreated cells or IL-1 β-treated cells in the presence of α-thrombin. The expression of inducible nitric oxide synthase mRNA and protein in vascular smooth muscle cells by IL-1 β was impaired by α-thrombin. These results demonstrate that thrombin regulates the expression of the inducible nitric oxide synthase at a transcriptional level via the proteolytic activation of the thrombin receptor in vascular smooth muscle cells.
Persistent Identifierhttp://hdl.handle.net/10722/171173
ISSN
2015 Impact Factor: 5.255
2015 SCImago Journal Rankings: 2.089
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSchiniKerth, VBen_US
dc.contributor.authorFisslthaler, Ben_US
dc.contributor.authorAndersen, TTen_US
dc.contributor.authorFenton Ii, JWen_US
dc.contributor.authorVanhoutte, PMen_US
dc.contributor.authorBusse, Ren_US
dc.date.accessioned2012-10-30T06:12:31Z-
dc.date.available2012-10-30T06:12:31Z-
dc.date.issued1995en_US
dc.identifier.citationThrombosis And Haemostasis, 1995, v. 74 n. 3, p. 980-986en_US
dc.identifier.issn0340-6245en_US
dc.identifier.urihttp://hdl.handle.net/10722/171173-
dc.description.abstractProteolytically active forms of thrombin (α- and γ-thrombin) and thrombin receptor peptides inhibited the release of nitrite, a stable endproduct of nitric oxide, evoked by interleukin-1 β (IL-1 β) in cultured vascular smooth muscle cells while proteolytically inactive forms [D-Phe-Pro-Arg chloromethyl ketone-α-thrombin (PPACK-α-thrombin) and diisopropylphosphoryl-α-thrombin (DIP-α-thrombin)] had either no or only minimal inhibitory effects. Under bioassay conditions, perfusates from columns containing IL-1 β-activated vascular smooth muscle cells or cells treated with IL-1 β plus PPACK-α-thrombin relaxed detector blood vessels. These relaxations were abolished by the inhibitor of nitric oxide synthesis, N(G)-nitro-L-arginine. No relaxations were obtained with untreated cells or IL-1 β-treated cells in the presence of α-thrombin. The expression of inducible nitric oxide synthase mRNA and protein in vascular smooth muscle cells by IL-1 β was impaired by α-thrombin. These results demonstrate that thrombin regulates the expression of the inducible nitric oxide synthase at a transcriptional level via the proteolytic activation of the thrombin receptor in vascular smooth muscle cells.en_US
dc.languageengen_US
dc.publisherSchattauer GmbH. The Journal's web site is located at http://www.thrombosis-online.comen_US
dc.relation.ispartofThrombosis and Haemostasisen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Assayen_US
dc.subject.meshCatalysisen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEndopeptidases - Metabolismen_US
dc.subject.meshEnzyme Induction - Drug Effectsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMuscle, Smooth, Vascular - Cytology - Drug Effects - Enzymologyen_US
dc.subject.meshNitric Oxide - Biosynthesisen_US
dc.subject.meshNitric Oxide Synthase - Biosynthesis - Geneticsen_US
dc.subject.meshNitrites - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshReceptors, Thrombin - Drug Effectsen_US
dc.subject.meshThrombin - Pharmacologyen_US
dc.subject.meshTranscription, Genetic - Drug Effectsen_US
dc.titleThrombin prevents the expression of inducible nitric oxide synthase in vascular smooth muscle cells by a proteolytically-activated thrombin receptoren_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8571333-
dc.identifier.scopuseid_2-s2.0-0029161946en_US
dc.identifier.volume74en_US
dc.identifier.issue3en_US
dc.identifier.spage980en_US
dc.identifier.epage986en_US
dc.identifier.isiWOS:A1995RV50400031-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridSchiniKerth, VB=7003640666en_US
dc.identifier.scopusauthoridFisslthaler, B=6603859835en_US
dc.identifier.scopusauthoridAndersen, TT=13310400200en_US
dc.identifier.scopusauthoridFenton II, JW=7202310341en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US
dc.identifier.scopusauthoridBusse, R=7102661981en_US

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