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Article: Alteration of lysophosphatidylcholine content in low density lipoprotein after oxidative modification: Relationship to endothelium dependent relaxation

TitleAlteration of lysophosphatidylcholine content in low density lipoprotein after oxidative modification: Relationship to endothelium dependent relaxation
Authors
Issue Date1994
PublisherOxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.org
Citation
Cardiovascular Research, 1994, v. 28 n. 10, p. 1476-1481 How to Cite?
AbstractObjective: The aim was to examine the formation of lipid peroxidation products and the alteration in phospholipid content in low density lipoprotein (LDL) after oxidative modification by CuSO 4, and subsequently, to determine the ability of the modified LDL to impair endothelium dependent relaxation in rat aortic rings. Methods: Blood samples were obtained from normal human volunteers. LDL was prepared by sequential ultracentrifugation and it was oxidatively modified in the presence of 5 μM CuSO 4. Lipid peroxidation products (thiobarbituric acid reactive substances, TBARS), and alterations in electrophoretic mobility and phospholipid content were determined in normal (native) and oxidised LDL. Endothelium dependent relaxation was produced by acetylcholine (10 -8 - 10 -5 M) in phenylephrine precontracted rat aortic rings. Results: LDL incubated for 24 h with 5 μM CuSO 4 at 20°C and 37°C with constant agitation displayed higher amounts of TBARS than the respective native LDL. While the amounts of TBARS in LDL modified at 20°C and 37°C were similar, the former condition resulted in statistically smaller changes of phospholipid contents. LDL with higher lysophosphatidylcholine content showed greater impairment of endothelium dependent relaxation in rat aortic rings than LDL with lower lysophosphatidylcholine content. Conclusions: The raised lysophosphatidylcholine level in oxidatively modified LDL was related to the ability of the LDL to impair endothelium dependent relaxation. However, lipid peroxidation products assessed by TBARS did not relate to the phospholipid changes in LDL and therefore cannot be used to predict the vascular effects of LDL after oxidative modification.
Persistent Identifierhttp://hdl.handle.net/10722/171121
ISSN
2015 Impact Factor: 5.465
2015 SCImago Journal Rankings: 2.897
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, SYen_US
dc.contributor.authorLu, Xen_US
dc.contributor.authorChoy, Sen_US
dc.contributor.authorDembinski, TCen_US
dc.contributor.authorHatch, GMen_US
dc.contributor.authorMymin, Den_US
dc.contributor.authorShen, Xen_US
dc.contributor.authorAngel, Aen_US
dc.contributor.authorChoy, PCen_US
dc.contributor.authorMan, RYKen_US
dc.date.accessioned2012-10-30T06:12:16Z-
dc.date.available2012-10-30T06:12:16Z-
dc.date.issued1994en_US
dc.identifier.citationCardiovascular Research, 1994, v. 28 n. 10, p. 1476-1481en_US
dc.identifier.issn0008-6363en_US
dc.identifier.urihttp://hdl.handle.net/10722/171121-
dc.description.abstractObjective: The aim was to examine the formation of lipid peroxidation products and the alteration in phospholipid content in low density lipoprotein (LDL) after oxidative modification by CuSO 4, and subsequently, to determine the ability of the modified LDL to impair endothelium dependent relaxation in rat aortic rings. Methods: Blood samples were obtained from normal human volunteers. LDL was prepared by sequential ultracentrifugation and it was oxidatively modified in the presence of 5 μM CuSO 4. Lipid peroxidation products (thiobarbituric acid reactive substances, TBARS), and alterations in electrophoretic mobility and phospholipid content were determined in normal (native) and oxidised LDL. Endothelium dependent relaxation was produced by acetylcholine (10 -8 - 10 -5 M) in phenylephrine precontracted rat aortic rings. Results: LDL incubated for 24 h with 5 μM CuSO 4 at 20°C and 37°C with constant agitation displayed higher amounts of TBARS than the respective native LDL. While the amounts of TBARS in LDL modified at 20°C and 37°C were similar, the former condition resulted in statistically smaller changes of phospholipid contents. LDL with higher lysophosphatidylcholine content showed greater impairment of endothelium dependent relaxation in rat aortic rings than LDL with lower lysophosphatidylcholine content. Conclusions: The raised lysophosphatidylcholine level in oxidatively modified LDL was related to the ability of the LDL to impair endothelium dependent relaxation. However, lipid peroxidation products assessed by TBARS did not relate to the phospholipid changes in LDL and therefore cannot be used to predict the vascular effects of LDL after oxidative modification.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.orgen_US
dc.relation.ispartofCardiovascular Researchen_US
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAortaen_US
dc.subject.meshCopper - Pharmacologyen_US
dc.subject.meshCopper Sulfateen_US
dc.subject.meshElectrophoresisen_US
dc.subject.meshEndothelium, Vascular - Drug Effects - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLipid Peroxidationen_US
dc.subject.meshLipoproteins, Ldl - Metabolism - Pharmacologyen_US
dc.subject.meshLysophosphatidylcholines - Metabolismen_US
dc.subject.meshNitric Oxide - Metabolismen_US
dc.subject.meshPhenylephrine - Pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.titleAlteration of lysophosphatidylcholine content in low density lipoprotein after oxidative modification: Relationship to endothelium dependent relaxationen_US
dc.typeArticleen_US
dc.identifier.emailMan, RYK:rykman@hkucc.hku.hken_US
dc.identifier.authorityMan, RYK=rp00236en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/cvr/28.10.1476-
dc.identifier.pmid8001034-
dc.identifier.scopuseid_2-s2.0-0027996669en_US
dc.identifier.volume28en_US
dc.identifier.issue10en_US
dc.identifier.spage1476en_US
dc.identifier.epage1481en_US
dc.identifier.isiWOS:A1994PL80000004-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLiu, SY=24822482600en_US
dc.identifier.scopusauthoridLu, X=7404839457en_US
dc.identifier.scopusauthoridChoy, S=7004160363en_US
dc.identifier.scopusauthoridDembinski, TC=6602725224en_US
dc.identifier.scopusauthoridHatch, GM=7102271713en_US
dc.identifier.scopusauthoridMymin, D=6701628222en_US
dc.identifier.scopusauthoridShen, X=25226368300en_US
dc.identifier.scopusauthoridAngel, A=7101766569en_US
dc.identifier.scopusauthoridChoy, PC=7006633002en_US
dc.identifier.scopusauthoridMan, RYK=7004986435en_US

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