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Article: Endothelium-dependent contractions to oxygen-derived free radicals in the canine basilar artery

TitleEndothelium-dependent contractions to oxygen-derived free radicals in the canine basilar artery
Authors
Issue Date1993
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Heart And Circulatory Physiology, 1993, v. 264 n. 3 33-3, p. H859-H864 How to Cite?
AbstractExperiments were designed to determine the effect of oxygen-derived free radicals in isolated canine basilar arteries. Rings with and without endothelium were suspended for isometric tension recording in modified Krebs- Ringer bicarbonate solution bubbled with 95% O 2-5% CO 2 (temperature = 37°C: pH = 7.4). A radioimmunoassay technique was used to measure production of prostaglandins and thromboxane B 2. Xanthine oxidase (1-9 mU/ml, in the presence of 10 -4 M xanthine) and hydrogen peroxide (10 -6 to 10 -4 M) caused concentration-dependent contractions. The removal of endothelium reversed these contractions into relaxations. Contractions to xanthine oxidase and hydrogen peroxide were inhibited in the presence of superoxide dismutase (150 U/ml), catalase (1,200 U/ml), indomethacin (10 -5 M), and SQ 29548 (10 -6 M) but not in the presence of deferoxamine (10 -4 to 10 -3 M) and dimethyl sulfoxide (10 -4 M). N(G)-monomethyl-L-arginine (3 x 10 -5 M) augmented the contractions to hydrogen peroxide. Xanthine oxidase stimulated production of 6-keto-prostaglandin F(1α), prostaglandin F(2α), prostaglandin E 2, and thromboxane B 2. The stimulatory effect was prevented by the removal of endothelial cells. These studies suggest that xanthine oxidase causes endothelium-dependent contractions mediated by 1) hydrogen peroxide-induced stimulation of the endothelial metabolism of arachidonic acid via the cyclooxygenase pathway, leading to activation of prostaglandin H 2-thromboxane A 2 receptors, and 2) inactivation of basal production of nitric oxide by superoxide anions.
Persistent Identifierhttp://hdl.handle.net/10722/171113
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKatusic, ZSen_US
dc.contributor.authorSchugel, Jen_US
dc.contributor.authorCosentino, Fen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:14Z-
dc.date.available2012-10-30T06:12:14Z-
dc.date.issued1993en_US
dc.identifier.citationAmerican Journal Of Physiology - Heart And Circulatory Physiology, 1993, v. 264 n. 3 33-3, p. H859-H864en_US
dc.identifier.issn0002-9513en_US
dc.identifier.urihttp://hdl.handle.net/10722/171113-
dc.description.abstractExperiments were designed to determine the effect of oxygen-derived free radicals in isolated canine basilar arteries. Rings with and without endothelium were suspended for isometric tension recording in modified Krebs- Ringer bicarbonate solution bubbled with 95% O 2-5% CO 2 (temperature = 37°C: pH = 7.4). A radioimmunoassay technique was used to measure production of prostaglandins and thromboxane B 2. Xanthine oxidase (1-9 mU/ml, in the presence of 10 -4 M xanthine) and hydrogen peroxide (10 -6 to 10 -4 M) caused concentration-dependent contractions. The removal of endothelium reversed these contractions into relaxations. Contractions to xanthine oxidase and hydrogen peroxide were inhibited in the presence of superoxide dismutase (150 U/ml), catalase (1,200 U/ml), indomethacin (10 -5 M), and SQ 29548 (10 -6 M) but not in the presence of deferoxamine (10 -4 to 10 -3 M) and dimethyl sulfoxide (10 -4 M). N(G)-monomethyl-L-arginine (3 x 10 -5 M) augmented the contractions to hydrogen peroxide. Xanthine oxidase stimulated production of 6-keto-prostaglandin F(1α), prostaglandin F(2α), prostaglandin E 2, and thromboxane B 2. The stimulatory effect was prevented by the removal of endothelial cells. These studies suggest that xanthine oxidase causes endothelium-dependent contractions mediated by 1) hydrogen peroxide-induced stimulation of the endothelial metabolism of arachidonic acid via the cyclooxygenase pathway, leading to activation of prostaglandin H 2-thromboxane A 2 receptors, and 2) inactivation of basal production of nitric oxide by superoxide anions.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Heart and Circulatory Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArginine - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshBasilar Artery - Drug Effects - Physiologyen_US
dc.subject.meshCattleen_US
dc.subject.meshDogsen_US
dc.subject.meshEndothelium, Vascular - Physiologyen_US
dc.subject.meshFree Radicalsen_US
dc.subject.meshHydrogen Peroxide - Pharmacologyen_US
dc.subject.meshIndomethacin - Pharmacologyen_US
dc.subject.meshMeclofenamic Acid - Pharmacologyen_US
dc.subject.meshMuscle Contraction - Drug Effects - Physiologyen_US
dc.subject.meshMuscle, Smooth, Vascular - Drug Effects - Physiologyen_US
dc.subject.meshOxygen - Metabolismen_US
dc.subject.meshProstaglandins - Biosynthesisen_US
dc.subject.meshSuperoxide Dismutase - Pharmacologyen_US
dc.subject.meshThromboxane B2 - Biosynthesisen_US
dc.subject.meshXanthineen_US
dc.subject.meshXanthine Oxidase - Pharmacologyen_US
dc.subject.meshXanthines - Pharmacologyen_US
dc.subject.meshOmega-N-Methylarginineen_US
dc.titleEndothelium-dependent contractions to oxygen-derived free radicals in the canine basilar arteryen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8456988-
dc.identifier.scopuseid_2-s2.0-0027535482en_US
dc.identifier.volume264en_US
dc.identifier.issue3 33-3en_US
dc.identifier.spageH859en_US
dc.identifier.epageH864en_US
dc.identifier.isiWOS:A1993KV27100027-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKatusic, ZS=7006971465en_US
dc.identifier.scopusauthoridSchugel, J=55309841200en_US
dc.identifier.scopusauthoridCosentino, F=7006332290en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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