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Article: Thrombin inhibits induction of nitric oxide synthase in vascular smooth muscle cells

TitleThrombin inhibits induction of nitric oxide synthase in vascular smooth muscle cells
Authors
Issue Date1993
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Heart And Circulatory Physiology, 1993, v. 264 n. 2 33-2, p. H611-H616 How to Cite?
AbstractExperiments were designed to examine whether thrombin affects the production of nitric oxide-like factor(s) evoked by interleukin-1β (IL-1β) in cultured smooth muscle cells from the rat aorta. IL-1β stimulated the release of nitrite (a stable oxidation product of nitric oxide) from cultured smooth muscle cells. Thrombin inhibited in a concentration-dependent manner the release of nitrite caused by IL-1β. The inhibition was prevented by hirudin (a thrombin inhibitor) and required the presence of thrombin before or during the induction period. Under bioassay conditions, the perfusates from columns containing IL-1β-treated smooth muscle cells relaxed detector rat aortic rings without endothelium. The addition of IL-1β-treated smooth muscle cells to suspensions of indomethacin-treated platelets inhibited their aggregation. Control untreated smooth muscle cells or cells treated with thrombin alone did not have such effects. The treatment of smooth muscle cells with IL-1β in combination with thrombin blunted both the relaxing activities of the perfusates under bioassay conditions and the inhibition of platelet aggregation. These observations indicate that thrombin inhibits the production of nitric oxide-like factor(s) evoked by the inducible nitric oxide synthase in cultured smooth muscle cells from rat aorta.
Persistent Identifierhttp://hdl.handle.net/10722/171108
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSchini, VBen_US
dc.contributor.authorCatovsky, Sen_US
dc.contributor.authorDurante, Wen_US
dc.contributor.authorScottBurden, Ten_US
dc.contributor.authorSchafer, AIen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:13Z-
dc.date.available2012-10-30T06:12:13Z-
dc.date.issued1993en_US
dc.identifier.citationAmerican Journal Of Physiology - Heart And Circulatory Physiology, 1993, v. 264 n. 2 33-2, p. H611-H616en_US
dc.identifier.issn0002-9513en_US
dc.identifier.urihttp://hdl.handle.net/10722/171108-
dc.description.abstractExperiments were designed to examine whether thrombin affects the production of nitric oxide-like factor(s) evoked by interleukin-1β (IL-1β) in cultured smooth muscle cells from the rat aorta. IL-1β stimulated the release of nitrite (a stable oxidation product of nitric oxide) from cultured smooth muscle cells. Thrombin inhibited in a concentration-dependent manner the release of nitrite caused by IL-1β. The inhibition was prevented by hirudin (a thrombin inhibitor) and required the presence of thrombin before or during the induction period. Under bioassay conditions, the perfusates from columns containing IL-1β-treated smooth muscle cells relaxed detector rat aortic rings without endothelium. The addition of IL-1β-treated smooth muscle cells to suspensions of indomethacin-treated platelets inhibited their aggregation. Control untreated smooth muscle cells or cells treated with thrombin alone did not have such effects. The treatment of smooth muscle cells with IL-1β in combination with thrombin blunted both the relaxing activities of the perfusates under bioassay conditions and the inhibition of platelet aggregation. These observations indicate that thrombin inhibits the production of nitric oxide-like factor(s) evoked by the inducible nitric oxide synthase in cultured smooth muscle cells from rat aorta.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Heart and Circulatory Physiologyen_US
dc.subject.meshAmino Acid Oxidoreductases - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArginine - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEnzyme Inductionen_US
dc.subject.meshInterleukin-1 - Pharmacologyen_US
dc.subject.meshMuscle, Smooth, Vascular - Cytology - Enzymologyen_US
dc.subject.meshNitric Oxide Synthaseen_US
dc.subject.meshNitroarginineen_US
dc.subject.meshRatsen_US
dc.subject.meshThrombin - Pharmacologyen_US
dc.titleThrombin inhibits induction of nitric oxide synthase in vascular smooth muscle cellsen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7680540-
dc.identifier.scopuseid_2-s2.0-0027457667en_US
dc.identifier.volume264en_US
dc.identifier.issue2 33-2en_US
dc.identifier.spageH611en_US
dc.identifier.epageH616en_US
dc.identifier.isiWOS:A1993KN68100047-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSchini, VB=7004113565en_US
dc.identifier.scopusauthoridCatovsky, S=6602617293en_US
dc.identifier.scopusauthoridDurante, W=7006946922en_US
dc.identifier.scopusauthoridScottBurden, T=7004306459en_US
dc.identifier.scopusauthoridSchafer, AI=7202243976en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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