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Article: Guanosine 5'-O-(3-thiotriphosphate) causes endothelium-dependent, pertussis toxin-sensitive relaxations in porcine coronary arteries

TitleGuanosine 5'-O-(3-thiotriphosphate) causes endothelium-dependent, pertussis toxin-sensitive relaxations in porcine coronary arteries
Authors
Issue Date1992
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 1992, v. 189 n. 1, p. 324-329 How to Cite?
AbstractTo determine whether direct stimulation of endothelial G-proteins causes relaxations of the underlying vascular smooth muscle, the effects of guanosine 5'-O-(3-thiotriphosphate) (GTPγS) and sodium fluoride were studied in porcine coronary arteries and endothelial cells. Isometric tension was measured in coronary rings contracted with prostaglandin F(2α). GTPγS (in the presence of saponin) and sodium fluoride (in the presence of AlCl3) relaxed rings with, but not those without endothelium. The responses were inhibited by nitro-L-arginine and pertussis toxin. In membrane fractions of coronary endothelial cells, GTPγS and sodium fluoride inhibited the ADP-ribosylation of G-proteins catalyzed with [32P]-NAD and pertussis toxin. These data suggest that direct stimulation of G-proteins in endothelial cells by GTPγS and sodium fluoride causes a pertussis toxin-sensitive relaxation which may be attributed to the release of nitrix oxide.
Persistent Identifierhttp://hdl.handle.net/10722/171078
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShibano, Ten_US
dc.contributor.authorCodina, Jen_US
dc.contributor.authorBirnbaumer, Len_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:06Z-
dc.date.available2012-10-30T06:12:06Z-
dc.date.issued1992en_US
dc.identifier.citationBiochemical And Biophysical Research Communications, 1992, v. 189 n. 1, p. 324-329en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/171078-
dc.description.abstractTo determine whether direct stimulation of endothelial G-proteins causes relaxations of the underlying vascular smooth muscle, the effects of guanosine 5'-O-(3-thiotriphosphate) (GTPγS) and sodium fluoride were studied in porcine coronary arteries and endothelial cells. Isometric tension was measured in coronary rings contracted with prostaglandin F(2α). GTPγS (in the presence of saponin) and sodium fluoride (in the presence of AlCl3) relaxed rings with, but not those without endothelium. The responses were inhibited by nitro-L-arginine and pertussis toxin. In membrane fractions of coronary endothelial cells, GTPγS and sodium fluoride inhibited the ADP-ribosylation of G-proteins catalyzed with [32P]-NAD and pertussis toxin. These data suggest that direct stimulation of G-proteins in endothelial cells by GTPγS and sodium fluoride causes a pertussis toxin-sensitive relaxation which may be attributed to the release of nitrix oxide.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.subject.meshAdenosine Diphosphate Ribose - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArginine - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshCoronary Vessels - Drug Effects - Physiologyen_US
dc.subject.meshDinoprost - Pharmacologyen_US
dc.subject.meshEndothelium, Vascular - Physiologyen_US
dc.subject.meshGtp-Binding Proteins - Metabolismen_US
dc.subject.meshGuanosine 5'-O-(3-Thiotriphosphate) - Pharmacologyen_US
dc.subject.meshKineticsen_US
dc.subject.meshMuscle Relaxation - Drug Effectsen_US
dc.subject.meshMuscle, Smooth, Vascular - Drug Effects - Physiologyen_US
dc.subject.meshNitroarginineen_US
dc.subject.meshPertussis Toxinen_US
dc.subject.meshSaponins - Pharmacologyen_US
dc.subject.meshSodium Fluoride - Pharmacologyen_US
dc.subject.meshSwineen_US
dc.subject.meshVirulence Factors, Bordetella - Pharmacologyen_US
dc.titleGuanosine 5'-O-(3-thiotriphosphate) causes endothelium-dependent, pertussis toxin-sensitive relaxations in porcine coronary arteriesen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0006-291X(92)91561-4en_US
dc.identifier.pmid1449486-
dc.identifier.scopuseid_2-s2.0-0027055115en_US
dc.identifier.volume189en_US
dc.identifier.issue1en_US
dc.identifier.spage324en_US
dc.identifier.epage329en_US
dc.identifier.isiWOS:A1992KA51300046-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridShibano, T=7006946465en_US
dc.identifier.scopusauthoridCodina, J=7005011473en_US
dc.identifier.scopusauthoridBirnbaumer, L=35401284700en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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