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Article: Transforming growth factor-β1 inhibits L-arginine-derived relaxing factor(s) from smooth muscle cells

TitleTransforming growth factor-β1 inhibits L-arginine-derived relaxing factor(s) from smooth muscle cells
Authors
Issue Date1992
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Heart And Circulatory Physiology, 1992, v. 262 n. 6 31-6, p. H1788-H1795 How to Cite?
AbstractThe effects of human recombinant interleukin-1β were investigated on the release of nonprostanoid relaxing substances from cultured aortic smooth muscle cells from Wistar rats. Cells cultured on microcarrier beads were packed in columns. The perfusate over these beads was bioassayed by measuring changes in isometric tension of contracted arteries without endothelium. The perfusates from interleukin-1β-treated smooth muscle cells, but not from control cells, evoked relaxations. The relaxations persisted when the transit time between the cultured smooth muscle cells and the detector was increased to 5 min. The effect of relaxing substance(s) was inhibited by cycloheximide, nitro-L-arginine, methylene blue, and transforming growth factor-β1. L- Arginine but not D-arginine overcame the blockade by nitro-L-arginine. Superoxide dismutase potentiated the relaxations. In cells cultured in multiwell plates, interleukin-1β evoked a time- and concentration-dependent accumulation of nitrite in the extracellular medium that was inhibited dose dependently by transforming growth factor-β1. These studies demonstrate that cultured smooth muscle cells can be stimulated to produce nitric oxide- related substances and that the inducible pathway is modulated by transforming growth factor-β1.
Persistent Identifierhttp://hdl.handle.net/10722/171067
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102

 

DC FieldValueLanguage
dc.contributor.authorJunquero, DCen_US
dc.contributor.authorSchini, VBen_US
dc.contributor.authorScottBurden, Ten_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:04Z-
dc.date.available2012-10-30T06:12:04Z-
dc.date.issued1992en_US
dc.identifier.citationAmerican Journal Of Physiology - Heart And Circulatory Physiology, 1992, v. 262 n. 6 31-6, p. H1788-H1795en_US
dc.identifier.issn0002-9513en_US
dc.identifier.urihttp://hdl.handle.net/10722/171067-
dc.description.abstractThe effects of human recombinant interleukin-1β were investigated on the release of nonprostanoid relaxing substances from cultured aortic smooth muscle cells from Wistar rats. Cells cultured on microcarrier beads were packed in columns. The perfusate over these beads was bioassayed by measuring changes in isometric tension of contracted arteries without endothelium. The perfusates from interleukin-1β-treated smooth muscle cells, but not from control cells, evoked relaxations. The relaxations persisted when the transit time between the cultured smooth muscle cells and the detector was increased to 5 min. The effect of relaxing substance(s) was inhibited by cycloheximide, nitro-L-arginine, methylene blue, and transforming growth factor-β1. L- Arginine but not D-arginine overcame the blockade by nitro-L-arginine. Superoxide dismutase potentiated the relaxations. In cells cultured in multiwell plates, interleukin-1β evoked a time- and concentration-dependent accumulation of nitrite in the extracellular medium that was inhibited dose dependently by transforming growth factor-β1. These studies demonstrate that cultured smooth muscle cells can be stimulated to produce nitric oxide- related substances and that the inducible pathway is modulated by transforming growth factor-β1.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Heart and Circulatory Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArginine - Analogs & Derivatives - Metabolism - Pharmacologyen_US
dc.subject.meshBiological Assayen_US
dc.subject.meshInterleukin-1 - Pharmacologyen_US
dc.subject.meshMuscle, Smooth - Cytology - Metabolismen_US
dc.subject.meshNitrites - Metabolismen_US
dc.subject.meshNitroarginineen_US
dc.subject.meshStereoisomerismen_US
dc.subject.meshTransforming Growth Factor Beta - Pharmacologyen_US
dc.subject.meshVasodilator Agents - Antagonists & Inhibitors - Metabolismen_US
dc.titleTransforming growth factor-β1 inhibits L-arginine-derived relaxing factor(s) from smooth muscle cellsen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1621837-
dc.identifier.scopuseid_2-s2.0-0026695643en_US
dc.identifier.volume262en_US
dc.identifier.issue6 31-6en_US
dc.identifier.spageH1788en_US
dc.identifier.epageH1795en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridJunquero, DC=26643025500en_US
dc.identifier.scopusauthoridSchini, VB=7004113565en_US
dc.identifier.scopusauthoridScottBurden, T=7004306459en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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