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Article: Methods of cardiac preservation alter the function of the endothelium in porcine coronary arteries

TitleMethods of cardiac preservation alter the function of the endothelium in porcine coronary arteries
Authors
Issue Date1991
PublisherMosby, Inc. The Journal's web site is located at http://www.elsevier.com/locate/jtcvs
Citation
Journal Of Thoracic And Cardiovascular Surgery, 1991, v. 102 n. 6, p. 923-930 How to Cite?
AbstractThis study was undertaken to determine whether clinical methods for preservation and storage of hearts explanted for transplantation affect the responsiveness of coronary arteries to vasoactive agents. Porcine hearts were perfused with crystalloid or blood cardioplegic solution. Rings of coronary arteries were suspended in organ chambers for measurement of isometric force (1) immediately after perfusion and (2) after 5 hours' storage of the hearts at 4° C in the same cardioplegic solution (n = 6 in each group). The maximal contraction of the smooth muscle to potassium chloride, 40 mmol/L, was reduced significantly after perfusion with crystalloid cardioplegic solution (10.8 ± 1.2 gm) compared with blood cardioplegic solution (17.3 ± 0.8 gm) and nonperfused coronary arteries (control group 16.9 ± 1.8 gm). The sensitivity of the arteries with endothelium to the contractile effects of prostaglandin F(2α) increased after perfusion with crystalloid cardioplegic solution (ED50, [-log mol/L] 5.8 ± 0.04) compared with blood cardioplegic solution (5.3 ± 0.02) and the control group (5.7 ± 0.03). In addition, relaxations to the calcium ionophore A23187, bradykinin, and the α2-agonist BHT-920, which depend on the presence of endothelial cells, were significantly reduced after perfusion with crystalloid compared with blood cardioplegic solution or the control group. The responsiveness of the endothelium and smooth muscle after 5 hours' cold storage was unaltered in the blood cardioplegia group, whereas storage resulted in functional recovery in the crystalloid cardioplegia group, with the result that all groups were comparable. These data suggest an immediate and reversible change in vascular function with crystalloid cardioplegia, which was not apparent with blood cardioplegia.
Persistent Identifierhttp://hdl.handle.net/10722/171035
ISSN
2015 Impact Factor: 3.494
2015 SCImago Journal Rankings: 2.369
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNilsson, FNen_US
dc.contributor.authorMiller, VMen_US
dc.contributor.authorVanhoutte, PMen_US
dc.contributor.authorMcgregor, CGAen_US
dc.date.accessioned2012-10-30T06:11:55Z-
dc.date.available2012-10-30T06:11:55Z-
dc.date.issued1991en_US
dc.identifier.citationJournal Of Thoracic And Cardiovascular Surgery, 1991, v. 102 n. 6, p. 923-930en_US
dc.identifier.issn0022-5223en_US
dc.identifier.urihttp://hdl.handle.net/10722/171035-
dc.description.abstractThis study was undertaken to determine whether clinical methods for preservation and storage of hearts explanted for transplantation affect the responsiveness of coronary arteries to vasoactive agents. Porcine hearts were perfused with crystalloid or blood cardioplegic solution. Rings of coronary arteries were suspended in organ chambers for measurement of isometric force (1) immediately after perfusion and (2) after 5 hours' storage of the hearts at 4° C in the same cardioplegic solution (n = 6 in each group). The maximal contraction of the smooth muscle to potassium chloride, 40 mmol/L, was reduced significantly after perfusion with crystalloid cardioplegic solution (10.8 ± 1.2 gm) compared with blood cardioplegic solution (17.3 ± 0.8 gm) and nonperfused coronary arteries (control group 16.9 ± 1.8 gm). The sensitivity of the arteries with endothelium to the contractile effects of prostaglandin F(2α) increased after perfusion with crystalloid cardioplegic solution (ED50, [-log mol/L] 5.8 ± 0.04) compared with blood cardioplegic solution (5.3 ± 0.02) and the control group (5.7 ± 0.03). In addition, relaxations to the calcium ionophore A23187, bradykinin, and the α2-agonist BHT-920, which depend on the presence of endothelial cells, were significantly reduced after perfusion with crystalloid compared with blood cardioplegic solution or the control group. The responsiveness of the endothelium and smooth muscle after 5 hours' cold storage was unaltered in the blood cardioplegia group, whereas storage resulted in functional recovery in the crystalloid cardioplegia group, with the result that all groups were comparable. These data suggest an immediate and reversible change in vascular function with crystalloid cardioplegia, which was not apparent with blood cardioplegia.en_US
dc.languageengen_US
dc.publisherMosby, Inc. The Journal's web site is located at http://www.elsevier.com/locate/jtcvsen_US
dc.relation.ispartofJournal of Thoracic and Cardiovascular Surgeryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAzepines - Pharmacologyen_US
dc.subject.meshBicarbonates - Pharmacologyen_US
dc.subject.meshBradykinin - Pharmacologyen_US
dc.subject.meshCalcimycin - Pharmacologyen_US
dc.subject.meshCalcium Chloride - Pharmacologyen_US
dc.subject.meshCardioplegic Solutions - Pharmacologyen_US
dc.subject.meshCoronary Vessels - Drug Effectsen_US
dc.subject.meshDinoprost - Pharmacologyen_US
dc.subject.meshEndothelium, Vascular - Drug Effectsen_US
dc.subject.meshFemaleen_US
dc.subject.meshMagnesium - Pharmacologyen_US
dc.subject.meshMuscle Contraction - Drug Effectsen_US
dc.subject.meshPotassium Chloride - Pharmacologyen_US
dc.subject.meshSodium Chloride - Pharmacologyen_US
dc.subject.meshSwineen_US
dc.subject.meshTime Factorsen_US
dc.titleMethods of cardiac preservation alter the function of the endothelium in porcine coronary arteriesen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1960999-
dc.identifier.scopuseid_2-s2.0-0026319856en_US
dc.identifier.volume102en_US
dc.identifier.issue6en_US
dc.identifier.spage923en_US
dc.identifier.epage930en_US
dc.identifier.isiWOS:A1991GV17400017-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridNilsson, FN=7102632519en_US
dc.identifier.scopusauthoridMiller, VM=7201476816en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US
dc.identifier.scopusauthoridMcGregor, CGA=35394153900en_US

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