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Article: Interleukin-1 β induces the production of an L-arginine-derived relaxing factor from cultured smooth muscle cells from rat aorta

TitleInterleukin-1 β induces the production of an L-arginine-derived relaxing factor from cultured smooth muscle cells from rat aorta
Authors
Issue Date1991
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 1991, v. 176 n. 1, p. 114-121 How to Cite?
AbstractThe effect of interleukin-1 β on the production of non-prostanoid vasoactive factors by cultured rat aortic smooth muscle cells was investigated. Under bioassay conditions, the perfusate from a column of confluent cells grown on beads and treated with interleukin-1 β (1 ng/ml for 18 to 24 hr) abolished the contraction of a canine coronary ring without endothelium contracted by phenylephrine (1 μM), while the perfusate from control cells had no effect. The relaxing activity of the perfusate was observed when transit times were increased from 1 sec to 5 min. Nitro L-arginine (100 μM) reversed the relaxations and L-arginine stereoselectively restored the relaxations. Interleukin-1 β (1 ng/ml) evoked a time-dependent accumulation of cyclic GMP but not cyclic AMP in cultured smooth muscle cells. The transfer of fresh or stored (-70°C) conditioned culture medium from interleukin-1 β-treated cells but not from control cells, to cultured smooth muscle cells stimulated the production of cyclic GMP. These observations demonstrate that interleukin-1 β induces the production of transferable factor which relaxes vascular smooth muscle and stimulates the production of cyclic GMP.
Persistent Identifierhttp://hdl.handle.net/10722/171004
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSchini, VBen_US
dc.contributor.authorJunquero, DCen_US
dc.contributor.authorScottBurden, Ten_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:11:48Z-
dc.date.available2012-10-30T06:11:48Z-
dc.date.issued1991en_US
dc.identifier.citationBiochemical And Biophysical Research Communications, 1991, v. 176 n. 1, p. 114-121en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/171004-
dc.description.abstractThe effect of interleukin-1 β on the production of non-prostanoid vasoactive factors by cultured rat aortic smooth muscle cells was investigated. Under bioassay conditions, the perfusate from a column of confluent cells grown on beads and treated with interleukin-1 β (1 ng/ml for 18 to 24 hr) abolished the contraction of a canine coronary ring without endothelium contracted by phenylephrine (1 μM), while the perfusate from control cells had no effect. The relaxing activity of the perfusate was observed when transit times were increased from 1 sec to 5 min. Nitro L-arginine (100 μM) reversed the relaxations and L-arginine stereoselectively restored the relaxations. Interleukin-1 β (1 ng/ml) evoked a time-dependent accumulation of cyclic GMP but not cyclic AMP in cultured smooth muscle cells. The transfer of fresh or stored (-70°C) conditioned culture medium from interleukin-1 β-treated cells but not from control cells, to cultured smooth muscle cells stimulated the production of cyclic GMP. These observations demonstrate that interleukin-1 β induces the production of transferable factor which relaxes vascular smooth muscle and stimulates the production of cyclic GMP.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.subject.mesh1-Methyl-3-Isobutylxanthine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAorta, Thoracic - Drug Effects - Physiologyen_US
dc.subject.meshArginine - Metabolism - Pharmacologyen_US
dc.subject.meshBiological Assayen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCoronary Vessels - Drug Effects - Physiologyen_US
dc.subject.meshCyclic Gmp - Metabolismen_US
dc.subject.meshDogsen_US
dc.subject.meshIndomethacin - Pharmacologyen_US
dc.subject.meshInterleukin-1 - Pharmacologyen_US
dc.subject.meshKineticsen_US
dc.subject.meshMuscle, Smooth, Vascular - Drug Effects - Physiologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshStereoisomerismen_US
dc.subject.meshVasodilator Agents - Pharmacologyen_US
dc.titleInterleukin-1 β induces the production of an L-arginine-derived relaxing factor from cultured smooth muscle cells from rat aortaen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0006-291X(91)90897-G-
dc.identifier.pmid1708241-
dc.identifier.scopuseid_2-s2.0-0025768140en_US
dc.identifier.volume176en_US
dc.identifier.issue1en_US
dc.identifier.spage114en_US
dc.identifier.epage121en_US
dc.identifier.isiWOS:A1991FG32800018-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSchini, VB=7004113565en_US
dc.identifier.scopusauthoridJunquero, DC=26643025500en_US
dc.identifier.scopusauthoridScottBurden, T=7004306459en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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