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Article: Stimulation of cyclic GMP production in cultured endothelial cells of the pig by bradykinin, adenosine diphosphate, calcium ionophore A23187 and nitric oxide

TitleStimulation of cyclic GMP production in cultured endothelial cells of the pig by bradykinin, adenosine diphosphate, calcium ionophore A23187 and nitric oxide
Authors
Issue Date1990
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 1990, v. 101 n. 1, p. 152-156 How to Cite?
Abstract1. The effects of bradykinin, adenosine diphosphate, calcium ionophore A23187 and nitric oxide on the production of adenosine 3':5'-cyclic monophosphate (cyclic AMP) and guanosine 3':5'-cyclic monophosphate (cyclic GMP) were investigated in cultured aortic endothelial cells of the pig. 2. Bradykinin (10-7 M), adenosine diphosphate (3 x 10-5 M), nitric oxide (2 x 10-6 M) and A23187 (10-6 M) stimulated the production of cyclic GMP. This stimulation reached a maximum within 1 min and declined rapidly with the first three agonists whereas that induced by A23187 was long-lasting. 3. These concentrations of bradykinin, A23187 and nitric oxide had no effect on cyclic AMP production. However, adenosine diphosphate (3 x 10-5 M) slightly but significantly enhanced its production by about 1.7 fold. 4. The basal content of cyclic GMP in endothelial cells was significantly reduced by haemoglobin (10-5 M, a scavenger of endothelium-derived relaxing factor(s)) and methylene blue (10-5 M, and inhibitor of the activation of soluble guanylate cyclase) and was significantly enhanced by superoxide dismutase (500 u ml-1, a scavenger of superoxide anions). The basal content of cyclic GMP was not affected by N(G)-monomethyl-L-arginine (10-5 M, a specific inhibitor of the formation of nitric oxide from L-arginine) and was slightly but significantly increased by its D-enantiomer, N(G)-monomethyl-D-arginine. 5. The production of cyclic GMP stimulated by bradykinin, adenosine diphosphate, A23187 and nitric oxide was inhibited by haemoglobin (10-5 M) and methylene blue (10-5 M) but was unaffected by superoxide dismutase (500 u ml-1). 6. The production of cyclic GMP stimulated by bradykinin, adenosine diphosphate or A23187, but not that stimulated by nitric oxide, was significantly reduced by N(G)-monomethyl-L-arginine (10-5 M). The production of cyclic GMP evoked by nitric oxide, but not that induced by the other three agents, was enhanced significantly by N(G)-monomethyl-D-arginine by about 1.5 fold. 7. These data indicate that the endothelium-dependent vasodilators bradykinin, adenosine diphosphate and A23187 activate the production of cyclic GMP in endothelial cells via the synthesis of nitric oxide, which in turn stimulates the soluble guanylate cyclase.
Persistent Identifierhttp://hdl.handle.net/10722/170970
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBoulanger, Cen_US
dc.contributor.authorSchini, VBen_US
dc.contributor.authorMoncada, Sen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:11:39Z-
dc.date.available2012-10-30T06:11:39Z-
dc.date.issued1990en_US
dc.identifier.citationBritish Journal Of Pharmacology, 1990, v. 101 n. 1, p. 152-156en_US
dc.identifier.issn0007-1188en_US
dc.identifier.urihttp://hdl.handle.net/10722/170970-
dc.description.abstract1. The effects of bradykinin, adenosine diphosphate, calcium ionophore A23187 and nitric oxide on the production of adenosine 3':5'-cyclic monophosphate (cyclic AMP) and guanosine 3':5'-cyclic monophosphate (cyclic GMP) were investigated in cultured aortic endothelial cells of the pig. 2. Bradykinin (10-7 M), adenosine diphosphate (3 x 10-5 M), nitric oxide (2 x 10-6 M) and A23187 (10-6 M) stimulated the production of cyclic GMP. This stimulation reached a maximum within 1 min and declined rapidly with the first three agonists whereas that induced by A23187 was long-lasting. 3. These concentrations of bradykinin, A23187 and nitric oxide had no effect on cyclic AMP production. However, adenosine diphosphate (3 x 10-5 M) slightly but significantly enhanced its production by about 1.7 fold. 4. The basal content of cyclic GMP in endothelial cells was significantly reduced by haemoglobin (10-5 M, a scavenger of endothelium-derived relaxing factor(s)) and methylene blue (10-5 M, and inhibitor of the activation of soluble guanylate cyclase) and was significantly enhanced by superoxide dismutase (500 u ml-1, a scavenger of superoxide anions). The basal content of cyclic GMP was not affected by N(G)-monomethyl-L-arginine (10-5 M, a specific inhibitor of the formation of nitric oxide from L-arginine) and was slightly but significantly increased by its D-enantiomer, N(G)-monomethyl-D-arginine. 5. The production of cyclic GMP stimulated by bradykinin, adenosine diphosphate, A23187 and nitric oxide was inhibited by haemoglobin (10-5 M) and methylene blue (10-5 M) but was unaffected by superoxide dismutase (500 u ml-1). 6. The production of cyclic GMP stimulated by bradykinin, adenosine diphosphate or A23187, but not that stimulated by nitric oxide, was significantly reduced by N(G)-monomethyl-L-arginine (10-5 M). The production of cyclic GMP evoked by nitric oxide, but not that induced by the other three agents, was enhanced significantly by N(G)-monomethyl-D-arginine by about 1.5 fold. 7. These data indicate that the endothelium-dependent vasodilators bradykinin, adenosine diphosphate and A23187 activate the production of cyclic GMP in endothelial cells via the synthesis of nitric oxide, which in turn stimulates the soluble guanylate cyclase.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_US
dc.relation.ispartofBritish Journal of Pharmacologyen_US
dc.subject.meshAdenosine Diphosphate - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArginine - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshBradykinin - Pharmacologyen_US
dc.subject.meshCalcimycin - Pharmacologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshCyclic Gmp - Biosynthesisen_US
dc.subject.meshEndothelium, Vascular - Drug Effects - Metabolismen_US
dc.subject.meshKineticsen_US
dc.subject.meshMethylene Blue - Pharmacologyen_US
dc.subject.meshNitric Oxide - Biosynthesis - Pharmacologyen_US
dc.subject.meshOxyhemoglobins - Metabolismen_US
dc.subject.meshStimulation, Chemicalen_US
dc.subject.meshSuperoxide Dismutase - Metabolismen_US
dc.subject.meshSuperoxides - Metabolismen_US
dc.subject.meshSwineen_US
dc.subject.meshOmega-N-Methylarginineen_US
dc.titleStimulation of cyclic GMP production in cultured endothelial cells of the pig by bradykinin, adenosine diphosphate, calcium ionophore A23187 and nitric oxideen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1476-5381.1990.tb12105.x-
dc.identifier.pmid2178013-
dc.identifier.scopuseid_2-s2.0-0024998640en_US
dc.identifier.volume101en_US
dc.identifier.issue1en_US
dc.identifier.spage152en_US
dc.identifier.epage156en_US
dc.identifier.isiWOS:A1990DX64100030-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridBoulanger, C=7006599024en_US
dc.identifier.scopusauthoridSchini, VB=7004113565en_US
dc.identifier.scopusauthoridMoncada, S=7202884383en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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