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Article: Human eosinophil major basic protein causes hyperreactivity of respiratory smooth muscle. Role of the epithelium

TitleHuman eosinophil major basic protein causes hyperreactivity of respiratory smooth muscle. Role of the epithelium
Authors
Issue Date1988
PublisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.org
Citation
American Review Of Respiratory Disease, 1988, v. 138 n. 3, p. 685-688 How to Cite?
AbstractMajor basic protein (MBP), one of several cationic proteins associated with the eosinophil granule, is cytotoxic to respiratory epithelium and is present in the sputum of asthmatics and on damaged bronchial epithelium from patients dying of asthma. The present experiments were performed in order to determine the effects of MBP on the responsiveness of airway smooth muscle. Rings of guinea pig trachea, in some of which the epithelium had been gently removed by rubbing, were incubated for 5 h in modified Krebs-Ringer bicarbonate solution containing buffer or human MBP (100 μg/ml). The tracheal rings were then suspended for isometric tension recording in organ chambers filled with Krebs-Ringer solution containing indomethacin. MBP did not affect the reactivity of rings in which the epithelium had been removed, but significantly augmented that of unrubbed rings to acetylcholine and histamine. The results suggest that MBP, by inhibiting the function of epithelial but not smooth muscle cells, causes hyperreactivity of respiratory smooth muscle, which may contribute to the hyperreactivity observed in chronic respiratory disease.
Persistent Identifierhttp://hdl.handle.net/10722/170882
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFlavahan, NAen_US
dc.contributor.authorSlifman, NRen_US
dc.contributor.authorGleich, GJen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:11:16Z-
dc.date.available2012-10-30T06:11:16Z-
dc.date.issued1988en_US
dc.identifier.citationAmerican Review Of Respiratory Disease, 1988, v. 138 n. 3, p. 685-688en_US
dc.identifier.issn0003-0805en_US
dc.identifier.urihttp://hdl.handle.net/10722/170882-
dc.description.abstractMajor basic protein (MBP), one of several cationic proteins associated with the eosinophil granule, is cytotoxic to respiratory epithelium and is present in the sputum of asthmatics and on damaged bronchial epithelium from patients dying of asthma. The present experiments were performed in order to determine the effects of MBP on the responsiveness of airway smooth muscle. Rings of guinea pig trachea, in some of which the epithelium had been gently removed by rubbing, were incubated for 5 h in modified Krebs-Ringer bicarbonate solution containing buffer or human MBP (100 μg/ml). The tracheal rings were then suspended for isometric tension recording in organ chambers filled with Krebs-Ringer solution containing indomethacin. MBP did not affect the reactivity of rings in which the epithelium had been removed, but significantly augmented that of unrubbed rings to acetylcholine and histamine. The results suggest that MBP, by inhibiting the function of epithelial but not smooth muscle cells, causes hyperreactivity of respiratory smooth muscle, which may contribute to the hyperreactivity observed in chronic respiratory disease.en_US
dc.languageengen_US
dc.publisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.orgen_US
dc.relation.ispartofAmerican Review of Respiratory Diseaseen_US
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlood Proteins - Isolation & Purification - Pharmacologyen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshDrug Interactionsen_US
dc.subject.meshEosinophil Granule Proteinsen_US
dc.subject.meshEosinophils - Metabolismen_US
dc.subject.meshEpithelial Cellsen_US
dc.subject.meshEpithelium - Drug Effects - Physiologyen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshHistamine - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle Contraction - Drug Effectsen_US
dc.subject.meshMuscle, Smooth - Cytology - Drug Effects - Physiologyen_US
dc.subject.meshRibonucleasesen_US
dc.subject.meshTrachea - Cytology - Drug Effects - Physiologyen_US
dc.titleHuman eosinophil major basic protein causes hyperreactivity of respiratory smooth muscle. Role of the epitheliumen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1164/ajrccm/138.3.685-
dc.identifier.pmid3202421-
dc.identifier.scopuseid_2-s2.0-0023785820en_US
dc.identifier.volume138en_US
dc.identifier.issue3en_US
dc.identifier.spage685en_US
dc.identifier.epage688en_US
dc.identifier.isiWOS:A1988Q221600032-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridFlavahan, NA=7006398882en_US
dc.identifier.scopusauthoridSlifman, NR=6602923008en_US
dc.identifier.scopusauthoridGleich, GJ=34770421900en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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