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Article: Effect of cooling on alpha-1 and alpha-2, adrenergic responses in canine saphenous and femoral veins

TitleEffect of cooling on alpha-1 and alpha-2, adrenergic responses in canine saphenous and femoral veins
Authors
Issue Date1986
PublisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org
Citation
Journal Of Pharmacology And Experimental Therapeutics, 1986, v. 238 n. 1, p. 139-147 How to Cite?
AbstractExperiments were designed to determine the effect of cooling on alpha-1 and alpha-2 adrenergic responses in isolated canine veins. Rings saphenous and femoral veins were suspended for isometric tension recording in modified Krebs-Ringer bicarbonate solution, gassed with 95% O2 and 5% CO2. Cooling (from 37-24°C) augmented contractions to norepinephrine in saphenous but caused depression in femoral veins. Cooling (to 24° C) had no effect on alpha-1 adrenergic responses evoked by phenylephrine in saphenous veins but caused depression in femoral veins. Alpha-2 adrenergic responses produced by UK 14,304 were augmented by cooling in the saphenous but were virtually abolished by cooling in femoral veins. Cooling decreased the dissociation constant (i.e., increased affinity) of corynanthine for alpha-1 adrenoceptors in saphenous and femoral veins (approximately 3-fold), and the dissociation constant of rauwolscine for alpha-2 adrenoceptors in saphenous veins (approximately 7.5-fold). The influence of cooling on alpha adrenoceptor responsiveness was analyzed using computer-generated receptor-models. The results suggest that the differential sensitivity of cutaneous and deep blood vessels to cooling results from differences in efficiency of alpha-1 and alpha-2 adrenoceptor response coupling. In the saphenous vein, there is a large alpha-1 adrenoceptor reserve which buffers the alpha-1 adrenergic response from the inhibitory influence of cooling. This coupled with a cooling-induced increase in alpha-2 adrenoceptor affinity ensures that cooling augments the response to norepinephrine. In the femoral vein, there is no alpha-1 adrenoceptor reserve and cooling therefore depresses alpha-1 adrenergic responses. The alpha-2 adrenergic response is so inefficient in this blood vessel that an increase in alpha-2 adrenoceptor affinity cannot overcome the inhibitory effect of cooling. Therefore, in the femoral vein, cooling inhibits alpha-1 and alpha-2 adrenergic responses and, thus, depresses responses produced by norepinephrine.
Persistent Identifierhttp://hdl.handle.net/10722/170805
ISSN
2015 Impact Factor: 3.76
2015 SCImago Journal Rankings: 1.847
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFlavahan, NAen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:10:55Z-
dc.date.available2012-10-30T06:10:55Z-
dc.date.issued1986en_US
dc.identifier.citationJournal Of Pharmacology And Experimental Therapeutics, 1986, v. 238 n. 1, p. 139-147en_US
dc.identifier.issn0022-3565en_US
dc.identifier.urihttp://hdl.handle.net/10722/170805-
dc.description.abstractExperiments were designed to determine the effect of cooling on alpha-1 and alpha-2 adrenergic responses in isolated canine veins. Rings saphenous and femoral veins were suspended for isometric tension recording in modified Krebs-Ringer bicarbonate solution, gassed with 95% O2 and 5% CO2. Cooling (from 37-24°C) augmented contractions to norepinephrine in saphenous but caused depression in femoral veins. Cooling (to 24° C) had no effect on alpha-1 adrenergic responses evoked by phenylephrine in saphenous veins but caused depression in femoral veins. Alpha-2 adrenergic responses produced by UK 14,304 were augmented by cooling in the saphenous but were virtually abolished by cooling in femoral veins. Cooling decreased the dissociation constant (i.e., increased affinity) of corynanthine for alpha-1 adrenoceptors in saphenous and femoral veins (approximately 3-fold), and the dissociation constant of rauwolscine for alpha-2 adrenoceptors in saphenous veins (approximately 7.5-fold). The influence of cooling on alpha adrenoceptor responsiveness was analyzed using computer-generated receptor-models. The results suggest that the differential sensitivity of cutaneous and deep blood vessels to cooling results from differences in efficiency of alpha-1 and alpha-2 adrenoceptor response coupling. In the saphenous vein, there is a large alpha-1 adrenoceptor reserve which buffers the alpha-1 adrenergic response from the inhibitory influence of cooling. This coupled with a cooling-induced increase in alpha-2 adrenoceptor affinity ensures that cooling augments the response to norepinephrine. In the femoral vein, there is no alpha-1 adrenoceptor reserve and cooling therefore depresses alpha-1 adrenergic responses. The alpha-2 adrenergic response is so inefficient in this blood vessel that an increase in alpha-2 adrenoceptor affinity cannot overcome the inhibitory effect of cooling. Therefore, in the femoral vein, cooling inhibits alpha-1 and alpha-2 adrenergic responses and, thus, depresses responses produced by norepinephrine.en_US
dc.languageengen_US
dc.publisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.orgen_US
dc.relation.ispartofJournal of Pharmacology and Experimental Therapeuticsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAzepines - Pharmacologyen_US
dc.subject.meshCold Temperatureen_US
dc.subject.meshDogsen_US
dc.subject.meshFemaleen_US
dc.subject.meshFemoral Vein - Metabolismen_US
dc.subject.meshIsometric Contractionen_US
dc.subject.meshMaleen_US
dc.subject.meshMathematicsen_US
dc.subject.meshNorepinephrine - Pharmacologyen_US
dc.subject.meshPhenoxybenzamine - Pharmacologyen_US
dc.subject.meshQuinoxalines - Pharmacologyen_US
dc.subject.meshReceptors, Adrenergic, Alpha - Drug Effects - Physiologyen_US
dc.subject.meshSaphenous Vein - Metabolismen_US
dc.subject.meshTime Factorsen_US
dc.titleEffect of cooling on alpha-1 and alpha-2, adrenergic responses in canine saphenous and femoral veinsen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3014112-
dc.identifier.scopuseid_2-s2.0-0022453261en_US
dc.identifier.volume238en_US
dc.identifier.issue1en_US
dc.identifier.spage139en_US
dc.identifier.epage147en_US
dc.identifier.isiWOS:A1986D211300022-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridFlavahan, NA=7006398882en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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