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Article: A sensitive method for the quantitation of lysophosphatidylcholine in canine heart

TitleA sensitive method for the quantitation of lysophosphatidylcholine in canine heart
Authors
Issue Date1985
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jlr.org/
Citation
Journal Of Lipid Research, 1985, v. 26 n. 9, p. 1166-1169 How to Cite?
AbstractWe have developed a procedure for the determination of small amounts of lysophosphatidylcholine in cardiac tissue. Lysophosphatidylcholine from canine heart was separated from the major phospholipid by column chromatography, and then acetylated with labeled acetic anhydride. The acetylated lysophosphatidylcholine was isolated by thin-layer chromatography and the lysophosphatidylcholine content was calculated from the radioactivity associated wth the acetylated product. Although the sensitivity of the assay depends on the specific radioactivity of the acetic anhydride used, as low as 0.5 nmol of lysophospholipid in tissue samples can be readily quantitated. The results obtained from the control and ischemic canine cardiac tissues by this assay compares favorably with those obtained by lipid-phosphorus assay. The sensitivity and specificity of the present procedure allows us and other investigators to assay for lysophosphatidylcholine content in very small (10 mg wet weight) tissue samples.
Persistent Identifierhttp://hdl.handle.net/10722/170788
ISSN
2015 Impact Factor: 4.368
2015 SCImago Journal Rankings: 2.529
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWientzek, Men_US
dc.contributor.authorArthur, Gen_US
dc.contributor.authorMan, RYKen_US
dc.contributor.authorChoy, PCen_US
dc.date.accessioned2012-10-30T06:10:51Z-
dc.date.available2012-10-30T06:10:51Z-
dc.date.issued1985en_US
dc.identifier.citationJournal Of Lipid Research, 1985, v. 26 n. 9, p. 1166-1169en_US
dc.identifier.issn0022-2275en_US
dc.identifier.urihttp://hdl.handle.net/10722/170788-
dc.description.abstractWe have developed a procedure for the determination of small amounts of lysophosphatidylcholine in cardiac tissue. Lysophosphatidylcholine from canine heart was separated from the major phospholipid by column chromatography, and then acetylated with labeled acetic anhydride. The acetylated lysophosphatidylcholine was isolated by thin-layer chromatography and the lysophosphatidylcholine content was calculated from the radioactivity associated wth the acetylated product. Although the sensitivity of the assay depends on the specific radioactivity of the acetic anhydride used, as low as 0.5 nmol of lysophospholipid in tissue samples can be readily quantitated. The results obtained from the control and ischemic canine cardiac tissues by this assay compares favorably with those obtained by lipid-phosphorus assay. The sensitivity and specificity of the present procedure allows us and other investigators to assay for lysophosphatidylcholine content in very small (10 mg wet weight) tissue samples.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jlr.org/en_US
dc.relation.ispartofJournal of Lipid Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChromatography, Gas - Methodsen_US
dc.subject.meshChromatography, Thin Layer - Methodsen_US
dc.subject.meshDogsen_US
dc.subject.meshFemaleen_US
dc.subject.meshLysophosphatidylcholines - Analysisen_US
dc.subject.meshMaleen_US
dc.subject.meshMicrochemistryen_US
dc.subject.meshMyocardium - Analysisen_US
dc.subject.meshPhospholipids - Isolation & Purificationen_US
dc.subject.meshTritiumen_US
dc.subject.meshType C Phospholipasesen_US
dc.titleA sensitive method for the quantitation of lysophosphatidylcholine in canine hearten_US
dc.typeArticleen_US
dc.identifier.emailMan, RYK:rykman@hkucc.hku.hken_US
dc.identifier.authorityMan, RYK=rp00236en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid4067437-
dc.identifier.scopuseid_2-s2.0-0022183035en_US
dc.identifier.volume26en_US
dc.identifier.issue9en_US
dc.identifier.spage1166en_US
dc.identifier.epage1169en_US
dc.identifier.isiWOS:A1985ARE2900016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWientzek, M=6602842169en_US
dc.identifier.scopusauthoridArthur, G=7102560612en_US
dc.identifier.scopusauthoridMan, RYK=7004986435en_US
dc.identifier.scopusauthoridChoy, PC=7006633002en_US

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