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Article: Ouabain inhibits endothelium-dependent relaxations to arachidonic acid in canine coronary arteries

TitleOuabain inhibits endothelium-dependent relaxations to arachidonic acid in canine coronary arteries
Authors
Issue Date1985
PublisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org
Citation
Journal Of Pharmacology And Experimental Therapeutics, 1985, v. 235 n. 1, p. 81-86 How to Cite?
AbstractExperiments were designed to analyze the effects of ouabain on the actions of exogenous arachidonic acid on endothelial and vascular smooth muscle cells. Rings or strips were prepared from left circumflex canine coronary arteries and suspended for isometric tension recording in organ chambers filled with oxygenerated modified Krebs-Ringer-bicarbonate solution. During contractions evoked by prostaglandin F(2α), arachidonic acid caused relaxations both in the presence and the absence of endothelium. However, removal of the endothelium reduced its inhibitory action. Indomethacin prevented the relaxations in rings without endothelium, but did not affect the response to high doses (10-6 to 10-5 M) of arachidonic acid in preparations with endothelium. The inhibitor of lipoxygenase, nordihydroguaiaretic acid, had no effect on the inhibitory responses to arachidonic acid in rings with or without endothelium. Ouabain abolished both the endothelium-dependent and the direct relaxations to arachidonic acid. Endothelium-dependent relaxations in response to oleic acid, elaidic acid, adenosine diphosphate and thrombin were not affected by ouabain. In the presence of indomethacin, coronary artery strips without endothelium were relaxed by arachidonic acid only when layered (intimal surface against intimal surface) with a longitudinal strip with endothelium. In layered preparations, treatment of the intact longitudinal strip with ouabain before layering prevented the relaxation, whereas pretreatment of the strip without endothelium had no effect. These experiments demonstrate that: 1) arachidonic acid stimulates the release of a relaxing factor from the endothelial cells of canine coronary arteries which differs from prostaglandins; 2) ouabain prevents this effect by acting on endothelial cells; and 3) the selective effect of ouabain on endothelium-dependent relaxations induced by arachidonic acid indicates that it either stimulates the release of a factor different from that produced by other endothelium-dependent relaxing substances and/or causes the release of the same relaxing mediator by different means.
Persistent Identifierhttp://hdl.handle.net/10722/170775
ISSN
2015 Impact Factor: 3.76
2015 SCImago Journal Rankings: 1.847
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRubanyi, GMen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:10:48Z-
dc.date.available2012-10-30T06:10:48Z-
dc.date.issued1985en_US
dc.identifier.citationJournal Of Pharmacology And Experimental Therapeutics, 1985, v. 235 n. 1, p. 81-86en_US
dc.identifier.issn0022-3565en_US
dc.identifier.urihttp://hdl.handle.net/10722/170775-
dc.description.abstractExperiments were designed to analyze the effects of ouabain on the actions of exogenous arachidonic acid on endothelial and vascular smooth muscle cells. Rings or strips were prepared from left circumflex canine coronary arteries and suspended for isometric tension recording in organ chambers filled with oxygenerated modified Krebs-Ringer-bicarbonate solution. During contractions evoked by prostaglandin F(2α), arachidonic acid caused relaxations both in the presence and the absence of endothelium. However, removal of the endothelium reduced its inhibitory action. Indomethacin prevented the relaxations in rings without endothelium, but did not affect the response to high doses (10-6 to 10-5 M) of arachidonic acid in preparations with endothelium. The inhibitor of lipoxygenase, nordihydroguaiaretic acid, had no effect on the inhibitory responses to arachidonic acid in rings with or without endothelium. Ouabain abolished both the endothelium-dependent and the direct relaxations to arachidonic acid. Endothelium-dependent relaxations in response to oleic acid, elaidic acid, adenosine diphosphate and thrombin were not affected by ouabain. In the presence of indomethacin, coronary artery strips without endothelium were relaxed by arachidonic acid only when layered (intimal surface against intimal surface) with a longitudinal strip with endothelium. In layered preparations, treatment of the intact longitudinal strip with ouabain before layering prevented the relaxation, whereas pretreatment of the strip without endothelium had no effect. These experiments demonstrate that: 1) arachidonic acid stimulates the release of a relaxing factor from the endothelial cells of canine coronary arteries which differs from prostaglandins; 2) ouabain prevents this effect by acting on endothelial cells; and 3) the selective effect of ouabain on endothelium-dependent relaxations induced by arachidonic acid indicates that it either stimulates the release of a factor different from that produced by other endothelium-dependent relaxing substances and/or causes the release of the same relaxing mediator by different means.en_US
dc.languageengen_US
dc.publisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.orgen_US
dc.relation.ispartofJournal of Pharmacology and Experimental Therapeuticsen_US
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAdenosine Diphosphate - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArachidonic Aciden_US
dc.subject.meshArachidonic Acids - Pharmacologyen_US
dc.subject.meshCoronary Vessels - Drug Effectsen_US
dc.subject.meshDinoprosten_US
dc.subject.meshDogsen_US
dc.subject.meshEndothelium - Drug Effectsen_US
dc.subject.meshFemaleen_US
dc.subject.meshIndomethacin - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle Contraction - Drug Effectsen_US
dc.subject.meshMuscle Relaxation - Drug Effectsen_US
dc.subject.meshMuscle, Smooth, Vascular - Drug Effectsen_US
dc.subject.meshOuabain - Pharmacologyen_US
dc.subject.meshProstaglandins F - Pharmacologyen_US
dc.subject.meshThrombin - Pharmacologyen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshVasodilation - Drug Effectsen_US
dc.titleOuabain inhibits endothelium-dependent relaxations to arachidonic acid in canine coronary arteriesen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3930700-
dc.identifier.scopuseid_2-s2.0-0021933564en_US
dc.identifier.volume235en_US
dc.identifier.issue1en_US
dc.identifier.spage81en_US
dc.identifier.epage86en_US
dc.identifier.isiWOS:A1985ASE5000014-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridRubanyi, GM=7005517991en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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