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Article: Tissue accumulation and release of newly synthesized 3H-dopamine and 3H-noradrenaline in canine saphenous veins incubated with 3H-(-)-tyrosine

TitleTissue accumulation and release of newly synthesized 3H-dopamine and 3H-noradrenaline in canine saphenous veins incubated with 3H-(-)-tyrosine
Authors
Issue Date1983
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00210/index.htm
Citation
Naunyn-Schmiedeberg's Archives Of Pharmacology, 1983, v. 324 n. 1, p. 7-14 How to Cite?
AbstractExperiments were designed to study the release of newly synthesized catecholamines in the isolated canine saphenous vein. Unlabelled (-)-tyrosine did not affect the contractions caused by electrical stimulation or exogenous noradrenaline and did not influence the basal efflux and the stimulation-induced overflow of 3H-noradrenaline in veins, previously incubated with the radiolabelled transmitter. The precursor increased the stimulation-induced overflow of the principal intraneuronal metabolite of 3H-noradrenaline, 3H-3,4-dihydroxyphenylglycol, and augmented the concentration of endogenous dopamine. Available column chromatographic procedures were modified to measure small amounts of 3H-catecholamines in the presence of large concentrations of tritiated (-)-tyrosine. Incubation of isolated veins with 3H-(-)-tyrosine caused concentration- and time-dependent tissue accumulation of newly synthesized catecholamines; the amounts of 3H-noradrenaline and 3H-dopamine were roughly comparable and were augmented by raising the external K+ concentration. In isolated veins, first incubated with 3H-(-)-tyrosine, and then superfused, a small basal efflux of 3H-noradrenaline was detected. Electrical stimulation caused a frequency-dependent overflow of 3H-catecholamines consisting mainly of 3H-noradrenaline; a stimulation-evoked efflux of 3H-dopamine (10% of total 3H-catecholamines) could be detected only when long stimulation periods were applied. After a 70-min period of superfusion, the 3H-dopamine content of the tissues decreased while that of 3H-noradrenaline remained unchanged, irrespective of whether the tissues had been stimulated electrically or not. The present results show that the isolated canine saphenous vein can synthesize and release 3H-noradrenaline and 3H-dopamine when incubated with 3H-(-)-tyrosine.
Persistent Identifierhttp://hdl.handle.net/10722/170697
ISSN
2015 Impact Factor: 2.376
2015 SCImago Journal Rankings: 0.859
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBoels, PJen_US
dc.contributor.authorVerbeuren, TJen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:10:30Z-
dc.date.available2012-10-30T06:10:30Z-
dc.date.issued1983en_US
dc.identifier.citationNaunyn-Schmiedeberg's Archives Of Pharmacology, 1983, v. 324 n. 1, p. 7-14en_US
dc.identifier.issn0028-1298en_US
dc.identifier.urihttp://hdl.handle.net/10722/170697-
dc.description.abstractExperiments were designed to study the release of newly synthesized catecholamines in the isolated canine saphenous vein. Unlabelled (-)-tyrosine did not affect the contractions caused by electrical stimulation or exogenous noradrenaline and did not influence the basal efflux and the stimulation-induced overflow of 3H-noradrenaline in veins, previously incubated with the radiolabelled transmitter. The precursor increased the stimulation-induced overflow of the principal intraneuronal metabolite of 3H-noradrenaline, 3H-3,4-dihydroxyphenylglycol, and augmented the concentration of endogenous dopamine. Available column chromatographic procedures were modified to measure small amounts of 3H-catecholamines in the presence of large concentrations of tritiated (-)-tyrosine. Incubation of isolated veins with 3H-(-)-tyrosine caused concentration- and time-dependent tissue accumulation of newly synthesized catecholamines; the amounts of 3H-noradrenaline and 3H-dopamine were roughly comparable and were augmented by raising the external K+ concentration. In isolated veins, first incubated with 3H-(-)-tyrosine, and then superfused, a small basal efflux of 3H-noradrenaline was detected. Electrical stimulation caused a frequency-dependent overflow of 3H-catecholamines consisting mainly of 3H-noradrenaline; a stimulation-evoked efflux of 3H-dopamine (10% of total 3H-catecholamines) could be detected only when long stimulation periods were applied. After a 70-min period of superfusion, the 3H-dopamine content of the tissues decreased while that of 3H-noradrenaline remained unchanged, irrespective of whether the tissues had been stimulated electrically or not. The present results show that the isolated canine saphenous vein can synthesize and release 3H-noradrenaline and 3H-dopamine when incubated with 3H-(-)-tyrosine.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00210/index.htmen_US
dc.relation.ispartofNaunyn-Schmiedeberg's Archives of Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDogsen_US
dc.subject.meshDopamine - Metabolismen_US
dc.subject.meshElectric Stimulationen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle, Smooth, Vascular - Metabolismen_US
dc.subject.meshNorepinephrine - Metabolismen_US
dc.subject.meshPotassium - Pharmacologyen_US
dc.subject.meshSaphenous Vein - Metabolismen_US
dc.subject.meshTritium - Diagnostic Useen_US
dc.subject.meshTyrosine - Metabolism - Pharmacologyen_US
dc.titleTissue accumulation and release of newly synthesized 3H-dopamine and 3H-noradrenaline in canine saphenous veins incubated with 3H-(-)-tyrosineen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF00647831-
dc.identifier.pmid6633679-
dc.identifier.scopuseid_2-s2.0-0020637662en_US
dc.identifier.volume324en_US
dc.identifier.issue1en_US
dc.identifier.spage7en_US
dc.identifier.epage14en_US
dc.identifier.isiWOS:A1983RH99800002-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridBoels, PJ=6701763271en_US
dc.identifier.scopusauthoridVerbeuren, TJ=7007006534en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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