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Article: A simple and sensitive method for the determination of the biological activity of bleomycin using isolated rat liver nuclei

TitleA simple and sensitive method for the determination of the biological activity of bleomycin using isolated rat liver nuclei
Authors
Issue Date1980
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabio
Citation
Analytical Biochemistry, 1980, v. 108 n. 2, p. 306-312 How to Cite?
AbstractA simple biochemical method for the determination of bleomycin is presented. The assay is based upon a DNA polymerase response to DNA damage caused by bleomycin in an isolated rat liver nuclear system. In the presence of ascorbate, the assay is sufficiently accurate and reproducible to permit measurement of levels of bleomycin between 0.1 and 1.0 μM. The lowest detectable concentration is approximately 7.5 nM (10.5 ng/ml). In contrast to other reported assays, which require deproteinization of serum samples and other pretreatments prior to assay, this technique is directly applicable to small serum samples (20 μl). Since this assay is predicated upon the molecular mechanism of action of bleomycin, it should, therefore, provide a more meaningful and precise measure of biologic antitumor activity than other chemical methods.
Persistent Identifierhttp://hdl.handle.net/10722/170614
ISSN
2015 Impact Factor: 2.243
2015 SCImago Journal Rankings: 0.725
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTom, WMen_US
dc.contributor.authorLynch, WEen_US
dc.contributor.authorSartiano, GPen_US
dc.date.accessioned2012-10-30T06:10:08Z-
dc.date.available2012-10-30T06:10:08Z-
dc.date.issued1980en_US
dc.identifier.citationAnalytical Biochemistry, 1980, v. 108 n. 2, p. 306-312en_US
dc.identifier.issn0003-2697en_US
dc.identifier.urihttp://hdl.handle.net/10722/170614-
dc.description.abstractA simple biochemical method for the determination of bleomycin is presented. The assay is based upon a DNA polymerase response to DNA damage caused by bleomycin in an isolated rat liver nuclear system. In the presence of ascorbate, the assay is sufficiently accurate and reproducible to permit measurement of levels of bleomycin between 0.1 and 1.0 μM. The lowest detectable concentration is approximately 7.5 nM (10.5 ng/ml). In contrast to other reported assays, which require deproteinization of serum samples and other pretreatments prior to assay, this technique is directly applicable to small serum samples (20 μl). Since this assay is predicated upon the molecular mechanism of action of bleomycin, it should, therefore, provide a more meaningful and precise measure of biologic antitumor activity than other chemical methods.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabioen_US
dc.relation.ispartofAnalytical Biochemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAscorbic Acid - Pharmacologyen_US
dc.subject.meshBleomycin - Blood - Pharmacologyen_US
dc.subject.meshCell Nucleus - Metabolismen_US
dc.subject.meshDna-Directed Dna Polymerase - Metabolismen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshLiver - Drug Effects - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMethodsen_US
dc.subject.meshRatsen_US
dc.subject.meshSubcellular Fractions - Analysisen_US
dc.subject.meshThymine Nucleotides - Metabolismen_US
dc.titleA simple and sensitive method for the determination of the biological activity of bleomycin using isolated rat liver nucleien_US
dc.typeArticleen_US
dc.identifier.emailTom, WM:wmtoma@hkucc.hku.hken_US
dc.identifier.authorityTom, WM=rp00237en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0003-2697(80)90590-4-
dc.identifier.pmid6161566-
dc.identifier.scopuseid_2-s2.0-0019180469en_US
dc.identifier.volume108en_US
dc.identifier.issue2en_US
dc.identifier.spage306en_US
dc.identifier.epage312en_US
dc.identifier.isiWOS:A1980KT40200020-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridTom, WM=7003709519en_US
dc.identifier.scopusauthoridLynch, WE=7202572236en_US
dc.identifier.scopusauthoridSartiano, GP=7004572167en_US

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