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Article: Inhibition of sympathetic neurotransmission in canine blood vessels by adenosine and adenine nucleotides

TitleInhibition of sympathetic neurotransmission in canine blood vessels by adenosine and adenine nucleotides
Authors
Issue Date1977
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://circres.ahajournals.org
Citation
Circulation Research, 1977, v. 40 n. 2, p. 208-215 How to Cite?
AbstractAdenosine and the adenine nucleotides caused a greater relaxation of strips of canine saphenous vein and tibial artery when they had been contracted by nerve stimulation than by exogenous norepinephrine. An infusion of adenosine into the dogs' lateral saphenous vein, perfused at constant flow, caused a greater relaxation of this vein when constricted by electrical stimulation of the lumbar sympathetic chain than by exogenous norepinephrine. That this difference was due to inhibition by these compounds of the output of neurotransmitter from the sympathetic nerve endings was demonstrated by column chromatographic analysis of the radioactivity in the superfusion fluid of vein strips, previously incubated with tritiated norepinephrine. Both adenosine and adenosine triphosphate (10-5 M) reduced the efflux of 3H norepinephrine during nerve stimulation with electrical impulses. Adenosine also reduced the efflux caused by potassium (30 mM), but not that caused by tyramine (6 x 10-6M). Theophylline antagonized the inhibitory, effect of adenosine on the sympathetic neurotrasmission. We found that at 4 x 10-4M adenosine triphosphate still caused a decreased efflux of neurotransmitter during electrical stimulation, but with adenosine the 3H norepinephrine efflux no longer decreased and the overflow of deaminated compounds increased. Furthermore, the same concentration of adenosine increased the efflux of 3H norepinephrine and deaminated compounds in unstimulated strips, and the increase of 3H norepinephrine was enhanced after monoamine oxidase inhibition. Thus, we conclude that at higher concentrations adenosine increases the intraneuronal leakage of norepinephrine out of the storage vesicles.
Persistent Identifierhttp://hdl.handle.net/10722/170524
ISSN
2015 Impact Factor: 11.551
2015 SCImago Journal Rankings: 5.755
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorVerhaeghe, RHen_US
dc.contributor.authorVanhoutte, PMen_US
dc.contributor.authorShepherd, JTen_US
dc.date.accessioned2012-10-30T06:09:48Z-
dc.date.available2012-10-30T06:09:48Z-
dc.date.issued1977en_US
dc.identifier.citationCirculation Research, 1977, v. 40 n. 2, p. 208-215en_US
dc.identifier.issn0009-7330en_US
dc.identifier.urihttp://hdl.handle.net/10722/170524-
dc.description.abstractAdenosine and the adenine nucleotides caused a greater relaxation of strips of canine saphenous vein and tibial artery when they had been contracted by nerve stimulation than by exogenous norepinephrine. An infusion of adenosine into the dogs' lateral saphenous vein, perfused at constant flow, caused a greater relaxation of this vein when constricted by electrical stimulation of the lumbar sympathetic chain than by exogenous norepinephrine. That this difference was due to inhibition by these compounds of the output of neurotransmitter from the sympathetic nerve endings was demonstrated by column chromatographic analysis of the radioactivity in the superfusion fluid of vein strips, previously incubated with tritiated norepinephrine. Both adenosine and adenosine triphosphate (10-5 M) reduced the efflux of 3H norepinephrine during nerve stimulation with electrical impulses. Adenosine also reduced the efflux caused by potassium (30 mM), but not that caused by tyramine (6 x 10-6M). Theophylline antagonized the inhibitory, effect of adenosine on the sympathetic neurotrasmission. We found that at 4 x 10-4M adenosine triphosphate still caused a decreased efflux of neurotransmitter during electrical stimulation, but with adenosine the 3H norepinephrine efflux no longer decreased and the overflow of deaminated compounds increased. Furthermore, the same concentration of adenosine increased the efflux of 3H norepinephrine and deaminated compounds in unstimulated strips, and the increase of 3H norepinephrine was enhanced after monoamine oxidase inhibition. Thus, we conclude that at higher concentrations adenosine increases the intraneuronal leakage of norepinephrine out of the storage vesicles.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://circres.ahajournals.orgen_US
dc.relation.ispartofCirculation Researchen_US
dc.subject.meshAdenine Nucleotides - Pharmacologyen_US
dc.subject.meshAdenosine - Pharmacologyen_US
dc.subject.meshAdenosine Diphosphate - Pharmacologyen_US
dc.subject.meshAdenosine Triphosphate - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArteries - Metabolismen_US
dc.subject.meshDogsen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshElectric Stimulationen_US
dc.subject.meshHindlimb - Blood Supplyen_US
dc.subject.meshNorepinephrine - Metabolism - Pharmacologyen_US
dc.subject.meshSaphenous Vein - Metabolismen_US
dc.subject.meshSympathetic Nervous System - Drug Effectsen_US
dc.subject.meshSynaptic Transmission - Drug Effectsen_US
dc.subject.meshVasomotor System - Drug Effectsen_US
dc.titleInhibition of sympathetic neurotransmission in canine blood vessels by adenosine and adenine nucleotidesen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid191214-
dc.identifier.scopuseid_2-s2.0-0017332924en_US
dc.identifier.volume40en_US
dc.identifier.issue2en_US
dc.identifier.spage208en_US
dc.identifier.epage215en_US
dc.identifier.isiWOS:A1977CV41000015-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridVerhaeghe, RH=7006347736en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US
dc.identifier.scopusauthoridShepherd, JT=7401742522en_US

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