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Article: MHC expression kinetics and immunogenicity of mesenchymal stromal cells after short-term IFN-gamma challenge.

TitleMHC expression kinetics and immunogenicity of mesenchymal stromal cells after short-term IFN-gamma challenge.
Authors
Issue Date2008
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphem
Citation
Experimental Hematology, 2008, v. 36 n. 11, p. 1545-1555 How to Cite?
AbstractOBJECTIVE: Under the influence of interferon-gamma (IFN-gamma), mesenchymal stromal cells (MSCs) are conditional antigen-presenting cells, which have immunosuppressive potential. Apart from IFN-gamma upregulation of major histocompatibility complexes class I and II (MHC-I and MHC-II) expression, the underlying kinetics and mechanisms have not been described previously. This information is helpful to delineate how human MSCs can be modulated by IFN-gamma in different clinical scenarios. MATERIALS AND METHODS: Here, we demonstrated that IFN-gamma-treated MSCs underwent classical signal transduction pathway via phosphorylation of signal transducers and activators of transcription-1, activation of interferon regulatory factor-1, and class II transactivator comparable to that of primary human blood macrophages. RESULTS: IFN-gamma markedly induced expression of MHC-I instantly, while its effects on MHC-II were less dramatic and delayed up to 4 days. This is due to a slower intracellular transport of the MHC-II antigen to the membrane surface. More important is that MSCs showed a reduction in their proliferation by 50% without evidence of cell death after prolonged IFN-gamma treatment for 8 days. High-dose IFN-gamma-treated MSCs (500 U/mL) could initiate T-cell activation as indicated by expression of CD25 and proliferation of allogeneic T cells. CONCLUSIONS: The summative IFN-gamma effects will adversely affect the immunoprivilege status and lifespan of MSCs.
Persistent Identifierhttp://hdl.handle.net/10722/170408
ISSN
2015 Impact Factor: 2.303
2015 SCImago Journal Rankings: 1.341
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, WKen_US
dc.contributor.authorSikYin Lau, Aen_US
dc.contributor.authorChunBong Li, Jen_US
dc.contributor.authorKaWai Law, Hen_US
dc.contributor.authorLau, YLen_US
dc.contributor.authorChiFung Chan, Gen_US
dc.date.accessioned2012-10-30T06:08:13Z-
dc.date.available2012-10-30T06:08:13Z-
dc.date.issued2008en_US
dc.identifier.citationExperimental Hematology, 2008, v. 36 n. 11, p. 1545-1555en_US
dc.identifier.issn0301-472Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/170408-
dc.description.abstractOBJECTIVE: Under the influence of interferon-gamma (IFN-gamma), mesenchymal stromal cells (MSCs) are conditional antigen-presenting cells, which have immunosuppressive potential. Apart from IFN-gamma upregulation of major histocompatibility complexes class I and II (MHC-I and MHC-II) expression, the underlying kinetics and mechanisms have not been described previously. This information is helpful to delineate how human MSCs can be modulated by IFN-gamma in different clinical scenarios. MATERIALS AND METHODS: Here, we demonstrated that IFN-gamma-treated MSCs underwent classical signal transduction pathway via phosphorylation of signal transducers and activators of transcription-1, activation of interferon regulatory factor-1, and class II transactivator comparable to that of primary human blood macrophages. RESULTS: IFN-gamma markedly induced expression of MHC-I instantly, while its effects on MHC-II were less dramatic and delayed up to 4 days. This is due to a slower intracellular transport of the MHC-II antigen to the membrane surface. More important is that MSCs showed a reduction in their proliferation by 50% without evidence of cell death after prolonged IFN-gamma treatment for 8 days. High-dose IFN-gamma-treated MSCs (500 U/mL) could initiate T-cell activation as indicated by expression of CD25 and proliferation of allogeneic T cells. CONCLUSIONS: The summative IFN-gamma effects will adversely affect the immunoprivilege status and lifespan of MSCs.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphemen_US
dc.relation.ispartofExperimental hematologyen_US
dc.rightsExperimental Hematology. Copyright © Elsevier Inc.-
dc.subject.meshCell Proliferation - Drug Effectsen_US
dc.subject.meshHistocompatibility Antigens Class I - Analysisen_US
dc.subject.meshHistocompatibility Antigens Class Ii - Analysisen_US
dc.subject.meshHumansen_US
dc.subject.meshInterferon-Gamma - Pharmacologyen_US
dc.subject.meshLymphocyte Activationen_US
dc.subject.meshMesenchymal Stem Cells - Drug Effects - Immunologyen_US
dc.subject.meshT-Lymphocytes, Regulatory - Drug Effects - Immunologyen_US
dc.titleMHC expression kinetics and immunogenicity of mesenchymal stromal cells after short-term IFN-gamma challenge.en_US
dc.typeArticleen_US
dc.identifier.emailSikYin Lau, A:asylau@hku.hken_US
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_US
dc.identifier.emailChiFung Chan, G:gcfchan@hkucc.hku.hken_US
dc.identifier.authoritySikYin Lau, A=rp00474en_US
dc.identifier.authorityLau, YL=rp00361en_US
dc.identifier.authorityChiFung Chan, G=rp00431en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.exphem.2008.06.008-
dc.identifier.pmid18715686-
dc.identifier.scopuseid_2-s2.0-58149153178en_US
dc.identifier.hkuros153945-
dc.identifier.volume36en_US
dc.identifier.issue11en_US
dc.identifier.spage1545en_US
dc.identifier.epage1555en_US
dc.identifier.isiWOS:000260617300015-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChan, WK=35080149500en_US
dc.identifier.scopusauthoridSikYin Lau, A=7202626202en_US
dc.identifier.scopusauthoridChunBong Li, J=24544452200en_US
dc.identifier.scopusauthoridKaWai Law, H=24544482700en_US
dc.identifier.scopusauthoridLau, YL=7201403380en_US
dc.identifier.scopusauthoridChiFung Chan, G=16160154400en_US

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