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Article: SOX9 directly binds CREB as a novel synergism with the PKA pathway in BMP-2-induced osteochondrogenic differentiation

TitleSOX9 directly binds CREB as a novel synergism with the PKA pathway in BMP-2-induced osteochondrogenic differentiation
Authors
Issue Date2009
PublisherAmerican Society for Bone and Mineral Research. The Journal's web site is located at http://www.jbmr.org/view/0/index.html
Citation
Journal Of Bone And Mineral Research, 2009, v. 24 n. 5, p. 826-836 How to Cite?
AbstractSOX9 acts as a master transcription factor in osteochondrogenesis, and the phosphorylation by protein kinase A (PKA) has been shown to increase its DNA binding and transactivation activity. The PKA pathway is involved in the complex downstream signaling underlying the BMP-2-mediated osteochondrogenesis. This study therefore aimed at further analyzing the possible cross-talk between the SOX9 and the PKA regulation on the background of BMP-2 stimulation. It was first shown that the removal of the residues serine 64 and 211 of SOX9 diminished, but did not completely deplete, its stimulatory effect on the expression of both osteo- and chondrogenic markers. PKA activators and inhibitors increased and decreased the action of wildtype and mutated SOX9, respectively. Interestingly, the interplay of the SOX9 action with the PKA pathway was further shown to occur through direct physical association between SOX9 and CREB, a prototypical PKA downstream transcription factor. Moreover, the binding was shown to be an active biological event happening on BMP-2 stimulation. The C-terminal domain of SOX9 and amino acid residue serine at position 133 of CREB were identified to be involved in the interaction. The action of SOX9 was enhanced by overexpressing CREB. These results suggest that PKA signaling synergizes with SOX9 at the nuclear and Cytoplasmic levels to promote BMP-2-induced osteochondrogenic differentiation. © 2009 American Society for Bone and Mineral Research.
Persistent Identifierhttp://hdl.handle.net/10722/170141
ISSN
2015 Impact Factor: 5.622
2015 SCImago Journal Rankings: 2.773
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhao, Len_US
dc.contributor.authorLi, Gen_US
dc.contributor.authorZhou, GQen_US
dc.date.accessioned2012-10-30T06:05:33Z-
dc.date.available2012-10-30T06:05:33Z-
dc.date.issued2009en_US
dc.identifier.citationJournal Of Bone And Mineral Research, 2009, v. 24 n. 5, p. 826-836en_US
dc.identifier.issn0884-0431en_US
dc.identifier.urihttp://hdl.handle.net/10722/170141-
dc.description.abstractSOX9 acts as a master transcription factor in osteochondrogenesis, and the phosphorylation by protein kinase A (PKA) has been shown to increase its DNA binding and transactivation activity. The PKA pathway is involved in the complex downstream signaling underlying the BMP-2-mediated osteochondrogenesis. This study therefore aimed at further analyzing the possible cross-talk between the SOX9 and the PKA regulation on the background of BMP-2 stimulation. It was first shown that the removal of the residues serine 64 and 211 of SOX9 diminished, but did not completely deplete, its stimulatory effect on the expression of both osteo- and chondrogenic markers. PKA activators and inhibitors increased and decreased the action of wildtype and mutated SOX9, respectively. Interestingly, the interplay of the SOX9 action with the PKA pathway was further shown to occur through direct physical association between SOX9 and CREB, a prototypical PKA downstream transcription factor. Moreover, the binding was shown to be an active biological event happening on BMP-2 stimulation. The C-terminal domain of SOX9 and amino acid residue serine at position 133 of CREB were identified to be involved in the interaction. The action of SOX9 was enhanced by overexpressing CREB. These results suggest that PKA signaling synergizes with SOX9 at the nuclear and Cytoplasmic levels to promote BMP-2-induced osteochondrogenic differentiation. © 2009 American Society for Bone and Mineral Research.en_US
dc.languageengen_US
dc.publisherAmerican Society for Bone and Mineral Research. The Journal's web site is located at http://www.jbmr.org/view/0/index.htmlen_US
dc.relation.ispartofJournal of Bone and Mineral Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Markers - Metabolismen_US
dc.subject.meshBone Morphogenetic Protein 2 - Pharmacologyen_US
dc.subject.meshCell Differentiation - Drug Effectsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshChondrogenesis - Drug Effectsen_US
dc.subject.meshCyclic Amp Response Element-Binding Protein - Metabolismen_US
dc.subject.meshCyclic Amp-Dependent Protein Kinases - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshOsteogenesis - Drug Effectsen_US
dc.subject.meshPhosphorylation - Drug Effectsen_US
dc.subject.meshProtein Binding - Drug Effectsen_US
dc.subject.meshSox9 Transcription Factor - Metabolismen_US
dc.subject.meshSignal Transduction - Drug Effectsen_US
dc.titleSOX9 directly binds CREB as a novel synergism with the PKA pathway in BMP-2-induced osteochondrogenic differentiationen_US
dc.typeArticleen_US
dc.identifier.emailZhou, GQ:wormoscz@gmail.comen_US
dc.identifier.authorityZhou, GQ=rp00527en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1359/jbmr.081236en_US
dc.identifier.pmid19113914-
dc.identifier.scopuseid_2-s2.0-65949089831en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-65949089831&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume24en_US
dc.identifier.issue5en_US
dc.identifier.spage826en_US
dc.identifier.epage836en_US
dc.identifier.isiWOS:000265550000010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZhao, L=7404456036en_US
dc.identifier.scopusauthoridLi, G=36014758500en_US
dc.identifier.scopusauthoridZhou, GQ=23394245100en_US

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