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Article: The co-transplantation of human bone marrow stromal cells and embryo olfactory ensheathing cells as a new approach to treat spinal cord injury in a rat model

TitleThe co-transplantation of human bone marrow stromal cells and embryo olfactory ensheathing cells as a new approach to treat spinal cord injury in a rat model
Authors
Issue Date2008
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/14653249.asp
Citation
Cytotherapy, 2008, v. 10 n. 6, p. 551-564 How to Cite?
AbstractBackground: Both bone marrow stromal cells (BMSC) and olfactory ensheathing cells (OEC) have been demonstrated experimentally as promising for therapy of spinal cord injury (SCI). However, clinical use may be constrained by the margin neuronal differentiation capacity of BMSC as well as the limited number of isolatable OEC. This study therefore tested the efficacy of co-grafting human BMSC and OEC in treating thoracic SCI. Methods: Rat SCI models were created with cushion forces. OEC were labeled with Hoechst 33342 and BMSC with BrdU or GFP. BMSC, OEC and BMSC plus OEC were injected into the injured sites of rat spinal cords. Histologic, electrophysiologic and functional approaches were applied to assess the effects of transplantation of these cell types. Results: Behavioral evaluation showed an improvement in animals with all cell-based treatments. The co-graft led to significantly higher gait scaling. The latency of transcranial magnetic motor-evoked potential (tcMMEP) responses was also better restored in the co-graft group. Larger numbers and sizes of axon bundles through the transitional zone between the normal and injured regions were observed in the co-graft animals in comparison with all other animals. Transplanted bone marrow stromal cells were identified as neurofilament-positive in the co-grafted animals although the number of glial fibrillary acidic protein-positive cells remained the same in all groups. Discussion: Taken together, our results suggest that the combined use of BMSC and OEC may provide an improved approach for the treatment of SCI.
Persistent Identifierhttp://hdl.handle.net/10722/170133
ISSN
2015 Impact Factor: 3.625
2015 SCImago Journal Rankings: 1.259
References

 

DC FieldValueLanguage
dc.contributor.authorDeng, YBen_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorZhu, WBen_US
dc.contributor.authorBi, XBen_US
dc.contributor.authorWang, YZen_US
dc.contributor.authorYe, MHen_US
dc.contributor.authorZhou, GQen_US
dc.date.accessioned2012-10-30T06:05:30Z-
dc.date.available2012-10-30T06:05:30Z-
dc.date.issued2008en_US
dc.identifier.citationCytotherapy, 2008, v. 10 n. 6, p. 551-564en_US
dc.identifier.issn1465-3249en_US
dc.identifier.urihttp://hdl.handle.net/10722/170133-
dc.description.abstractBackground: Both bone marrow stromal cells (BMSC) and olfactory ensheathing cells (OEC) have been demonstrated experimentally as promising for therapy of spinal cord injury (SCI). However, clinical use may be constrained by the margin neuronal differentiation capacity of BMSC as well as the limited number of isolatable OEC. This study therefore tested the efficacy of co-grafting human BMSC and OEC in treating thoracic SCI. Methods: Rat SCI models were created with cushion forces. OEC were labeled with Hoechst 33342 and BMSC with BrdU or GFP. BMSC, OEC and BMSC plus OEC were injected into the injured sites of rat spinal cords. Histologic, electrophysiologic and functional approaches were applied to assess the effects of transplantation of these cell types. Results: Behavioral evaluation showed an improvement in animals with all cell-based treatments. The co-graft led to significantly higher gait scaling. The latency of transcranial magnetic motor-evoked potential (tcMMEP) responses was also better restored in the co-graft group. Larger numbers and sizes of axon bundles through the transitional zone between the normal and injured regions were observed in the co-graft animals in comparison with all other animals. Transplanted bone marrow stromal cells were identified as neurofilament-positive in the co-grafted animals although the number of glial fibrillary acidic protein-positive cells remained the same in all groups. Discussion: Taken together, our results suggest that the combined use of BMSC and OEC may provide an improved approach for the treatment of SCI.en_US
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/14653249.aspen_US
dc.relation.ispartofCytotherapyen_US
dc.rightsCytotherapy. Copyright © Informa Healthcare.-
dc.subject.meshAnimalsen_US
dc.subject.meshAxons - Physiologyen_US
dc.subject.meshBone Marrow Cells - Cytology - Physiologyen_US
dc.subject.meshBone Marrow Transplantationen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshOlfactory Bulb - Cytology - Physiology - Transplantationen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSpinal Cord Injuries - Surgeryen_US
dc.subject.meshStem Cell Transplantationen_US
dc.subject.meshStromal Cells - Cytology - Physiology - Transplantationen_US
dc.titleThe co-transplantation of human bone marrow stromal cells and embryo olfactory ensheathing cells as a new approach to treat spinal cord injury in a rat modelen_US
dc.typeArticleen_US
dc.identifier.emailZhou, GQ:wormoscz@gmail.comen_US
dc.identifier.authorityZhou, GQ=rp00527en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1080/14653240802165673en_US
dc.identifier.pmid18608352-
dc.identifier.scopuseid_2-s2.0-53749094964en_US
dc.identifier.hkuros144002-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-53749094964&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume10en_US
dc.identifier.issue6en_US
dc.identifier.spage551en_US
dc.identifier.epage564en_US
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridDeng, YB=15032713100en_US
dc.identifier.scopusauthoridLiu, Y=8943983800en_US
dc.identifier.scopusauthoridZhu, WB=35207905200en_US
dc.identifier.scopusauthoridBi, XB=25227509200en_US
dc.identifier.scopusauthoridWang, YZ=7601489365en_US
dc.identifier.scopusauthoridYe, MH=7202049055en_US
dc.identifier.scopusauthoridZhou, GQ=23394245100en_US

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