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Article: Implantation of BM mesenchymal stem cells into injured spinal cord elicits de novo neurogenesis and functional recovery: Evidence from a study in rhesus monkeys

TitleImplantation of BM mesenchymal stem cells into injured spinal cord elicits de novo neurogenesis and functional recovery: Evidence from a study in rhesus monkeys
Authors
Issue Date2006
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/14653249.asp
Citation
Cytotherapy, 2006, v. 8 n. 3, p. 210-214 How to Cite?
Abstract
Background: Transplantation of mesenchymal stem cells (MSC) in rodent models has proved to be an effective therapeutic approach for spinal cord injury (SCI). However, further studies in primate models are still needed before clinical application of MSC to patients. Methods: MSC were isolated from rhesus monkey BM and induced ex vivo to differentiate into neural lineage cells. Induced cells were labeled with Hoechst 33342 and injected into the injured sites of rhesus SCI models. Function of the injured spinal cord was assessed using Tarlov behavior assessment, sensory responses and electrophysiologic tests of cortical somatosensory-evoked potential (CSEP) and motor-evoked potential (MEP). In vivo differentiation of the implanted cells was demonstrated by the presence of neural cell markers in Hoechst 33342-labeled cells. The re-establishment of the axonal pathway was demonstrated using a true blue (TB) chloride retrograde tracing study. Results: Monkeys achieved Tarlov grades 2-3 and nearly normal sensory responses 3 months after cell transplantation. Both CSEP and MEP showed recovery features. The presence of the neural cell markers neurofilament (NF), neuro-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was observed in approximately 10% of Hoechst 33342-labeled cells. TB, originally injected at the caudal side of injured sites, was traceable in the rostral thoracic spinal cord, red nucleus and sensory motor cortex. Discussion: Our results suggest that the implantation of MSC-derived cells elicits de novo neurogenesis and functional recovery in a non-human primate SCI model and should harness the clinical application of BM MSC in SCI patients. © 2006 ISCT.
Persistent Identifierhttp://hdl.handle.net/10722/170086
ISSN
2013 Impact Factor: 3.100
ISI Accession Number ID
References

 

Author Affiliations
  1. Queen's University Belfast
  2. Sun Yat-Sen University
DC FieldValueLanguage
dc.contributor.authorDeng, YBen_US
dc.contributor.authorLiu, XGen_US
dc.contributor.authorLiu, ZGen_US
dc.contributor.authorLiu, XLen_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorZhou, GQen_US
dc.date.accessioned2012-10-30T06:05:14Z-
dc.date.available2012-10-30T06:05:14Z-
dc.date.issued2006en_US
dc.identifier.citationCytotherapy, 2006, v. 8 n. 3, p. 210-214en_US
dc.identifier.issn1465-3249en_US
dc.identifier.urihttp://hdl.handle.net/10722/170086-
dc.description.abstractBackground: Transplantation of mesenchymal stem cells (MSC) in rodent models has proved to be an effective therapeutic approach for spinal cord injury (SCI). However, further studies in primate models are still needed before clinical application of MSC to patients. Methods: MSC were isolated from rhesus monkey BM and induced ex vivo to differentiate into neural lineage cells. Induced cells were labeled with Hoechst 33342 and injected into the injured sites of rhesus SCI models. Function of the injured spinal cord was assessed using Tarlov behavior assessment, sensory responses and electrophysiologic tests of cortical somatosensory-evoked potential (CSEP) and motor-evoked potential (MEP). In vivo differentiation of the implanted cells was demonstrated by the presence of neural cell markers in Hoechst 33342-labeled cells. The re-establishment of the axonal pathway was demonstrated using a true blue (TB) chloride retrograde tracing study. Results: Monkeys achieved Tarlov grades 2-3 and nearly normal sensory responses 3 months after cell transplantation. Both CSEP and MEP showed recovery features. The presence of the neural cell markers neurofilament (NF), neuro-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was observed in approximately 10% of Hoechst 33342-labeled cells. TB, originally injected at the caudal side of injured sites, was traceable in the rostral thoracic spinal cord, red nucleus and sensory motor cortex. Discussion: Our results suggest that the implantation of MSC-derived cells elicits de novo neurogenesis and functional recovery in a non-human primate SCI model and should harness the clinical application of BM MSC in SCI patients. © 2006 ISCT.en_US
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/14653249.aspen_US
dc.relation.ispartofCytotherapyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cd - Analysisen_US
dc.subject.meshBone Marrow Cells - Cytologyen_US
dc.subject.meshCell Culture Techniquesen_US
dc.subject.meshCell Differentiation - Drug Effectsen_US
dc.subject.meshCell Separationen_US
dc.subject.meshDrugs, Chinese Herbal - Pharmacologyen_US
dc.subject.meshElectrophysiologyen_US
dc.subject.meshEvoked Potentials, Motor - Physiologyen_US
dc.subject.meshEvoked Potentials, Somatosensory - Physiologyen_US
dc.subject.meshGene Expression - Geneticsen_US
dc.subject.meshGlutamate Decarboxylase - Geneticsen_US
dc.subject.meshIsoenzymes - Geneticsen_US
dc.subject.meshMacaca Mulattaen_US
dc.subject.meshMaleen_US
dc.subject.meshMesenchymal Stem Cell Transplantation - Methodsen_US
dc.subject.meshMesenchymal Stem Cells - Chemistry - Cytology - Metabolismen_US
dc.subject.meshNerve Regenerationen_US
dc.subject.meshPhenanthrenes - Pharmacologyen_US
dc.subject.meshRecovery Of Functionen_US
dc.subject.meshSpinal Cord Injuries - Physiopathology - Therapyen_US
dc.subject.meshTreatment Outcomeen_US
dc.titleImplantation of BM mesenchymal stem cells into injured spinal cord elicits de novo neurogenesis and functional recovery: Evidence from a study in rhesus monkeysen_US
dc.typeArticleen_US
dc.identifier.emailZhou, GQ:wormoscz@gmail.comen_US
dc.identifier.authorityZhou, GQ=rp00527en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1080/14653240600760808en_US
dc.identifier.pmid16793730en_US
dc.identifier.scopuseid_2-s2.0-33745456034en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33745456034&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume8en_US
dc.identifier.issue3en_US
dc.identifier.spage210en_US
dc.identifier.epage214en_US
dc.identifier.isiWOS:000238504500005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridDeng, YB=15032713100en_US
dc.identifier.scopusauthoridLiu, XG=8853081400en_US
dc.identifier.scopusauthoridLiu, ZG=26031511400en_US
dc.identifier.scopusauthoridLiu, XL=8911849600en_US
dc.identifier.scopusauthoridLiu, Y=36012292400en_US
dc.identifier.scopusauthoridZhou, GQ=23394245100en_US
dc.identifier.citeulike710678-

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