Article: Implantation of BM mesenchymal stem cells into injured spinal cord elicits de novo neurogenesis and functional recovery: Evidence from a study in rhesus monkeys

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TitleImplantation of BM mesenchymal stem cells into injured spinal cord elicits de novo neurogenesis and functional recovery: Evidence from a study in rhesus monkeys
AuthorsDeng, YB2
Liu, XG2
Liu, ZG2
Liu, XL2
Liu, Y2
Zhou, GQ1
Issue Date2006
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/14653249.asp
CitationCytotherapy, 2006, v. 8 n. 3, p. 210-214 [How to Cite?]
DOI: http://dx.doi.org/10.1080/14653240600760808
AbstractBackground: Transplantation of mesenchymal stem cells (MSC) in rodent models has proved to be an effective therapeutic approach for spinal cord injury (SCI). However, further studies in primate models are still needed before clinical application of MSC to patients. Methods: MSC were isolated from rhesus monkey BM and induced ex vivo to differentiate into neural lineage cells. Induced cells were labeled with Hoechst 33342 and injected into the injured sites of rhesus SCI models. Function of the injured spinal cord was assessed using Tarlov behavior assessment, sensory responses and electrophysiologic tests of cortical somatosensory-evoked potential (CSEP) and motor-evoked potential (MEP). In vivo differentiation of the implanted cells was demonstrated by the presence of neural cell markers in Hoechst 33342-labeled cells. The re-establishment of the axonal pathway was demonstrated using a true blue (TB) chloride retrograde tracing study. Results: Monkeys achieved Tarlov grades 2-3 and nearly normal sensory responses 3 months after cell transplantation. Both CSEP and MEP showed recovery features. The presence of the neural cell markers neurofilament (NF), neuro-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was observed in approximately 10% of Hoechst 33342-labeled cells. TB, originally injected at the caudal side of injured sites, was traceable in the rostral thoracic spinal cord, red nucleus and sensory motor cortex. Discussion: Our results suggest that the implantation of MSC-derived cells elicits de novo neurogenesis and functional recovery in a non-human primate SCI model and should harness the clinical application of BM MSC in SCI patients. © 2006 ISCT.
ISSN1465-3249
2011 Impact Factor: 3.627
2011 SCImago Journal Rankings: 0.330
DOIhttp://dx.doi.org/10.1080/14653240600760808
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorDeng, YB
dc.contributor.authorLiu, XG
dc.contributor.authorLiu, ZG
dc.contributor.authorLiu, XL
dc.contributor.authorLiu, Y
dc.contributor.authorZhou, GQ
dc.date.accessioned2012-10-30T06:05:14Z
dc.date.available2012-10-30T06:05:14Z
dc.date.issued2006
dc.description.abstractBackground: Transplantation of mesenchymal stem cells (MSC) in rodent models has proved to be an effective therapeutic approach for spinal cord injury (SCI). However, further studies in primate models are still needed before clinical application of MSC to patients. Methods: MSC were isolated from rhesus monkey BM and induced ex vivo to differentiate into neural lineage cells. Induced cells were labeled with Hoechst 33342 and injected into the injured sites of rhesus SCI models. Function of the injured spinal cord was assessed using Tarlov behavior assessment, sensory responses and electrophysiologic tests of cortical somatosensory-evoked potential (CSEP) and motor-evoked potential (MEP). In vivo differentiation of the implanted cells was demonstrated by the presence of neural cell markers in Hoechst 33342-labeled cells. The re-establishment of the axonal pathway was demonstrated using a true blue (TB) chloride retrograde tracing study. Results: Monkeys achieved Tarlov grades 2-3 and nearly normal sensory responses 3 months after cell transplantation. Both CSEP and MEP showed recovery features. The presence of the neural cell markers neurofilament (NF), neuro-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was observed in approximately 10% of Hoechst 33342-labeled cells. TB, originally injected at the caudal side of injured sites, was traceable in the rostral thoracic spinal cord, red nucleus and sensory motor cortex. Discussion: Our results suggest that the implantation of MSC-derived cells elicits de novo neurogenesis and functional recovery in a non-human primate SCI model and should harness the clinical application of BM MSC in SCI patients. © 2006 ISCT.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCytotherapy, 2006, v. 8 n. 3, p. 210-214 [How to Cite?]
DOI: http://dx.doi.org/10.1080/14653240600760808
dc.identifier.citeulike710678
dc.identifier.doihttp://dx.doi.org/10.1080/14653240600760808
dc.identifier.epage214
dc.identifier.issn1465-3249
2011 Impact Factor: 3.627
2011 SCImago Journal Rankings: 0.330
dc.identifier.issue3
dc.identifier.pmid16793730
dc.identifier.scopuseid_2-s2.0-33745456034
dc.identifier.spage210
dc.identifier.urihttp://hdl.handle.net/10722/170086
dc.identifier.volume8
dc.languageeng
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/14653249.asp
dc.publisher.placeUnited Kingdom
dc.relation.ispartofCytotherapy
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshAntigens, Cd - Analysis
dc.subject.meshBone Marrow Cells - Cytology
dc.subject.meshCell Culture Techniques
dc.subject.meshCell Differentiation - Drug Effects
dc.subject.meshCell Separation
dc.subject.meshDrugs, Chinese Herbal - Pharmacology
dc.subject.meshElectrophysiology
dc.subject.meshEvoked Potentials, Motor - Physiology
dc.subject.meshEvoked Potentials, Somatosensory - Physiology
dc.subject.meshGene Expression - Genetics
dc.subject.meshGlutamate Decarboxylase - Genetics
dc.subject.meshIsoenzymes - Genetics
dc.subject.meshMacaca Mulatta
dc.subject.meshMale
dc.subject.meshMesenchymal Stem Cell Transplantation - Methods
dc.subject.meshMesenchymal Stem Cells - Chemistry - Cytology - Metabolism
dc.subject.meshNerve Regeneration
dc.subject.meshPhenanthrenes - Pharmacology
dc.subject.meshRecovery Of Function
dc.subject.meshSpinal Cord Injuries - Physiopathology - Therapy
dc.subject.meshTreatment Outcome
dc.titleImplantation of BM mesenchymal stem cells into injured spinal cord elicits de novo neurogenesis and functional recovery: Evidence from a study in rhesus monkeys
dc.typeArticle
Author Affiliations
  1. Queen's University Belfast
  2. Sun Yat-Sen University