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Article: Implantation of BM mesenchymal stem cells into injured spinal cord elicits de novo neurogenesis and functional recovery: Evidence from a study in rhesus monkeys
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TitleImplantation of BM mesenchymal stem cells into injured spinal cord elicits de novo neurogenesis and functional recovery: Evidence from a study in rhesus monkeys
 
AuthorsDeng, YB2
Liu, XG2
Liu, ZG2
Liu, XL2
Liu, Y2
Zhou, GQ1
 
Issue Date2006
 
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/14653249.asp
 
CitationCytotherapy, 2006, v. 8 n. 3, p. 210-214 [How to Cite?]
DOI: http://dx.doi.org/10.1080/14653240600760808
 
AbstractBackground: Transplantation of mesenchymal stem cells (MSC) in rodent models has proved to be an effective therapeutic approach for spinal cord injury (SCI). However, further studies in primate models are still needed before clinical application of MSC to patients. Methods: MSC were isolated from rhesus monkey BM and induced ex vivo to differentiate into neural lineage cells. Induced cells were labeled with Hoechst 33342 and injected into the injured sites of rhesus SCI models. Function of the injured spinal cord was assessed using Tarlov behavior assessment, sensory responses and electrophysiologic tests of cortical somatosensory-evoked potential (CSEP) and motor-evoked potential (MEP). In vivo differentiation of the implanted cells was demonstrated by the presence of neural cell markers in Hoechst 33342-labeled cells. The re-establishment of the axonal pathway was demonstrated using a true blue (TB) chloride retrograde tracing study. Results: Monkeys achieved Tarlov grades 2-3 and nearly normal sensory responses 3 months after cell transplantation. Both CSEP and MEP showed recovery features. The presence of the neural cell markers neurofilament (NF), neuro-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was observed in approximately 10% of Hoechst 33342-labeled cells. TB, originally injected at the caudal side of injured sites, was traceable in the rostral thoracic spinal cord, red nucleus and sensory motor cortex. Discussion: Our results suggest that the implantation of MSC-derived cells elicits de novo neurogenesis and functional recovery in a non-human primate SCI model and should harness the clinical application of BM MSC in SCI patients. © 2006 ISCT.
 
ISSN1465-3249
2013 Impact Factor: 3.100
 
DOIhttp://dx.doi.org/10.1080/14653240600760808
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorDeng, YB
 
dc.contributor.authorLiu, XG
 
dc.contributor.authorLiu, ZG
 
dc.contributor.authorLiu, XL
 
dc.contributor.authorLiu, Y
 
dc.contributor.authorZhou, GQ
 
dc.date.accessioned2012-10-30T06:05:14Z
 
dc.date.available2012-10-30T06:05:14Z
 
dc.date.issued2006
 
dc.description.abstractBackground: Transplantation of mesenchymal stem cells (MSC) in rodent models has proved to be an effective therapeutic approach for spinal cord injury (SCI). However, further studies in primate models are still needed before clinical application of MSC to patients. Methods: MSC were isolated from rhesus monkey BM and induced ex vivo to differentiate into neural lineage cells. Induced cells were labeled with Hoechst 33342 and injected into the injured sites of rhesus SCI models. Function of the injured spinal cord was assessed using Tarlov behavior assessment, sensory responses and electrophysiologic tests of cortical somatosensory-evoked potential (CSEP) and motor-evoked potential (MEP). In vivo differentiation of the implanted cells was demonstrated by the presence of neural cell markers in Hoechst 33342-labeled cells. The re-establishment of the axonal pathway was demonstrated using a true blue (TB) chloride retrograde tracing study. Results: Monkeys achieved Tarlov grades 2-3 and nearly normal sensory responses 3 months after cell transplantation. Both CSEP and MEP showed recovery features. The presence of the neural cell markers neurofilament (NF), neuro-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was observed in approximately 10% of Hoechst 33342-labeled cells. TB, originally injected at the caudal side of injured sites, was traceable in the rostral thoracic spinal cord, red nucleus and sensory motor cortex. Discussion: Our results suggest that the implantation of MSC-derived cells elicits de novo neurogenesis and functional recovery in a non-human primate SCI model and should harness the clinical application of BM MSC in SCI patients. © 2006 ISCT.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationCytotherapy, 2006, v. 8 n. 3, p. 210-214 [How to Cite?]
DOI: http://dx.doi.org/10.1080/14653240600760808
 
dc.identifier.citeulike710678
 
dc.identifier.doihttp://dx.doi.org/10.1080/14653240600760808
 
dc.identifier.epage214
 
dc.identifier.issn1465-3249
2013 Impact Factor: 3.100
 
dc.identifier.issue3
 
dc.identifier.pmid16793730
 
dc.identifier.scopuseid_2-s2.0-33745456034
 
dc.identifier.spage210
 
dc.identifier.urihttp://hdl.handle.net/10722/170086
 
dc.identifier.volume8
 
dc.languageeng
 
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/14653249.asp
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofCytotherapy
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshAntigens, Cd - Analysis
 
dc.subject.meshBone Marrow Cells - Cytology
 
dc.subject.meshCell Culture Techniques
 
dc.subject.meshCell Differentiation - Drug Effects
 
dc.subject.meshCell Separation
 
dc.subject.meshDrugs, Chinese Herbal - Pharmacology
 
dc.subject.meshElectrophysiology
 
dc.subject.meshEvoked Potentials, Motor - Physiology
 
dc.subject.meshEvoked Potentials, Somatosensory - Physiology
 
dc.subject.meshGene Expression - Genetics
 
dc.subject.meshGlutamate Decarboxylase - Genetics
 
dc.subject.meshIsoenzymes - Genetics
 
dc.subject.meshMacaca Mulatta
 
dc.subject.meshMale
 
dc.subject.meshMesenchymal Stem Cell Transplantation - Methods
 
dc.subject.meshMesenchymal Stem Cells - Chemistry - Cytology - Metabolism
 
dc.subject.meshNerve Regeneration
 
dc.subject.meshPhenanthrenes - Pharmacology
 
dc.subject.meshRecovery Of Function
 
dc.subject.meshSpinal Cord Injuries - Physiopathology - Therapy
 
dc.subject.meshTreatment Outcome
 
dc.titleImplantation of BM mesenchymal stem cells into injured spinal cord elicits de novo neurogenesis and functional recovery: Evidence from a study in rhesus monkeys
 
dc.typeArticle
 
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<contributor.author>Liu, Y</contributor.author>
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<description.abstract>Background: Transplantation of mesenchymal stem cells (MSC) in rodent models has proved to be an effective therapeutic approach for spinal cord injury (SCI). However, further studies in primate models are still needed before clinical application of MSC to patients. Methods: MSC were isolated from rhesus monkey BM and induced ex vivo to differentiate into neural lineage cells. Induced cells were labeled with Hoechst 33342 and injected into the injured sites of rhesus SCI models. Function of the injured spinal cord was assessed using Tarlov behavior assessment, sensory responses and electrophysiologic tests of cortical somatosensory-evoked potential (CSEP) and motor-evoked potential (MEP). In vivo differentiation of the implanted cells was demonstrated by the presence of neural cell markers in Hoechst 33342-labeled cells. The re-establishment of the axonal pathway was demonstrated using a true blue (TB) chloride retrograde tracing study. Results: Monkeys achieved Tarlov grades 2-3 and nearly normal sensory responses 3 months after cell transplantation. Both CSEP and MEP showed recovery features. The presence of the neural cell markers neurofilament (NF), neuro-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was observed in approximately 10% of Hoechst 33342-labeled cells. TB, originally injected at the caudal side of injured sites, was traceable in the rostral thoracic spinal cord, red nucleus and sensory motor cortex. Discussion: Our results suggest that the implantation of MSC-derived cells elicits de novo neurogenesis and functional recovery in a non-human primate SCI model and should harness the clinical application of BM MSC in SCI patients. &#169; 2006 ISCT.</description.abstract>
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Author Affiliations
  1. Queen's University Belfast
  2. Sun Yat-Sen University