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Article: Effects of hydroxyapatite in 3-D chitosan-gelatin polymer network on human mesenchymal stem cell construct development

TitleEffects of hydroxyapatite in 3-D chitosan-gelatin polymer network on human mesenchymal stem cell construct development
Authors
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2006, v. 27 n. 9, p. 1859-1867 How to Cite?
AbstractHuman mesenchymal stem cells (hMSCs) have great potential in bone tissue engineering, and hydroxyapatite (HA), a natural component of human hard tissues, is believed to support hMSC growth and osteogenic differentiation. In this study, two types of biomimetic composite materials, chitosan-gelatin (CG) and hydroxyapatite/chitosan-gelatin (HCG), were fabricated and compared to examine the effects of HA on hMSC adhesion and 3-D construct development. The 2-D membranes were prepared to examine the influence of HA on adhesion efficiency of hMSCs, while 3-D porous scaffolds were produced to investigate the effects of HA on material adsorption properties and 3-D hMSC construct development. HA was found to promote protein and calcium ion adsorption of the 3-D porous scaffolds in the complete tissue culture media. HMSCs exhibited higher initial cell adhesion efficiency to 2-D HCG membranes, and maintained higher proliferation rates in the 3-D porous HCG than CG scaffolds with 3.3 times higher final DNA amount in HCG scaffolds over a 35-day period. Colony forming unit-fibroblast (CFU-F) assays showed that higher percentages of cells maintained their progenicity in the 3-D porous HCG scaffolds over the 35-day culture period. Differentiation assays indicated that the multi-lineage differentiation potential of the hMSCs was preserved in both 3-D porous scaffolds. However, higher alkaline phosphate activity was detected in the 3-D porous HCG scaffolds upon osteogenic induction indicating improved osteogenic differentiation potential. The results demonstrate that enhanced protein and calcium ion adsorption properties of HA in the CG polymer network improve initial cell adhesion and long-term growth, favor osteogenic differentiation upon induction, as well as maintain the progenicity of the 3-D hMSC constructs. © 2005 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/170078
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhao, Fen_US
dc.contributor.authorGrayson, WLen_US
dc.contributor.authorMa, Ten_US
dc.contributor.authorBunnell, Ben_US
dc.contributor.authorLu, WWen_US
dc.date.accessioned2012-10-30T06:05:11Z-
dc.date.available2012-10-30T06:05:11Z-
dc.date.issued2006en_US
dc.identifier.citationBiomaterials, 2006, v. 27 n. 9, p. 1859-1867en_US
dc.identifier.issn0142-9612en_US
dc.identifier.urihttp://hdl.handle.net/10722/170078-
dc.description.abstractHuman mesenchymal stem cells (hMSCs) have great potential in bone tissue engineering, and hydroxyapatite (HA), a natural component of human hard tissues, is believed to support hMSC growth and osteogenic differentiation. In this study, two types of biomimetic composite materials, chitosan-gelatin (CG) and hydroxyapatite/chitosan-gelatin (HCG), were fabricated and compared to examine the effects of HA on hMSC adhesion and 3-D construct development. The 2-D membranes were prepared to examine the influence of HA on adhesion efficiency of hMSCs, while 3-D porous scaffolds were produced to investigate the effects of HA on material adsorption properties and 3-D hMSC construct development. HA was found to promote protein and calcium ion adsorption of the 3-D porous scaffolds in the complete tissue culture media. HMSCs exhibited higher initial cell adhesion efficiency to 2-D HCG membranes, and maintained higher proliferation rates in the 3-D porous HCG than CG scaffolds with 3.3 times higher final DNA amount in HCG scaffolds over a 35-day period. Colony forming unit-fibroblast (CFU-F) assays showed that higher percentages of cells maintained their progenicity in the 3-D porous HCG scaffolds over the 35-day culture period. Differentiation assays indicated that the multi-lineage differentiation potential of the hMSCs was preserved in both 3-D porous scaffolds. However, higher alkaline phosphate activity was detected in the 3-D porous HCG scaffolds upon osteogenic induction indicating improved osteogenic differentiation potential. The results demonstrate that enhanced protein and calcium ion adsorption properties of HA in the CG polymer network improve initial cell adhesion and long-term growth, favor osteogenic differentiation upon induction, as well as maintain the progenicity of the 3-D hMSC constructs. © 2005 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterialsen_US
dc.relation.ispartofBiomaterialsen_US
dc.rightsBiomaterials. Copyright © Elsevier BV.-
dc.subject.meshBone Substitutesen_US
dc.subject.meshCell Adhesionen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Lineageen_US
dc.subject.meshChitosan - Chemistry - Pharmacologyen_US
dc.subject.meshDurapatite - Chemistry - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMesenchymal Stem Cells - Cytology - Drug Effectsen_US
dc.subject.meshTissue Engineering - Methodsen_US
dc.titleEffects of hydroxyapatite in 3-D chitosan-gelatin polymer network on human mesenchymal stem cell construct developmenten_US
dc.typeArticleen_US
dc.identifier.emailLu, WW:wwlu@hku.hken_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.biomaterials.2005.09.031en_US
dc.identifier.pmid16225916-
dc.identifier.scopuseid_2-s2.0-28744439514en_US
dc.identifier.hkuros121492-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-28744439514&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume27en_US
dc.identifier.issue9en_US
dc.identifier.spage1859en_US
dc.identifier.epage1867en_US
dc.identifier.isiWOS:000234962500023-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridZhao, F=36040646100en_US
dc.identifier.scopusauthoridGrayson, WL=9746362300en_US
dc.identifier.scopusauthoridMa, T=15923465900en_US
dc.identifier.scopusauthoridBunnell, B=35601772800en_US
dc.identifier.scopusauthoridLu, WW=7404215221en_US

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