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Article: Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds

TitlePreparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds
Authors
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2002, v. 23 n. 15, p. 3227-3234 How to Cite?
AbstractA novel biodegradable hydroxyapatite/chitosan-gelatin network (HA/CS-Gel) composite of similar composition to that of normal human bone was prepared as a three-dimensional biomimetic scaffold by phase separation method for bone tissue engineering. Changing the solid content and the compositional variables of the original mixtures allowed control of the porosities and densities of the scaffolds. The HA granules were dispersed uniformly in the organic network with intimate interface contact via pulverizing and ultrasonically treating commercial available HA particles. Scaffolds of 90.6% porosity were used to examine the proliferation and functions of the cells in this three-dimensional microenvironment by culturing neonatal rat caldaria osteoblasts. Histological and immunohistochemical staining and scanning electron microscopy observation indicated that the osteoblasts attached to and proliferated on the scaffolds. Extracellular matrices including collagen I and proteoglycan-like substrate were synthesized, while osteoid and bone-like tissue formed during the culture period. Furthermore, the cell/scaffold constructs had good biomineralization effect after 3 weeks in culture. © 2002 Elsevier Science Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/170041
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565
References

 

DC FieldValueLanguage
dc.contributor.authorZhao, Fen_US
dc.contributor.authorYin, Yen_US
dc.contributor.authorLu, WWen_US
dc.contributor.authorLeong, JCen_US
dc.contributor.authorZhang, Wen_US
dc.contributor.authorZhang, Jen_US
dc.contributor.authorZhang, Men_US
dc.contributor.authorYao, Ken_US
dc.date.accessioned2012-10-30T06:04:55Z-
dc.date.available2012-10-30T06:04:55Z-
dc.date.issued2002en_US
dc.identifier.citationBiomaterials, 2002, v. 23 n. 15, p. 3227-3234en_US
dc.identifier.issn0142-9612en_US
dc.identifier.urihttp://hdl.handle.net/10722/170041-
dc.description.abstractA novel biodegradable hydroxyapatite/chitosan-gelatin network (HA/CS-Gel) composite of similar composition to that of normal human bone was prepared as a three-dimensional biomimetic scaffold by phase separation method for bone tissue engineering. Changing the solid content and the compositional variables of the original mixtures allowed control of the porosities and densities of the scaffolds. The HA granules were dispersed uniformly in the organic network with intimate interface contact via pulverizing and ultrasonically treating commercial available HA particles. Scaffolds of 90.6% porosity were used to examine the proliferation and functions of the cells in this three-dimensional microenvironment by culturing neonatal rat caldaria osteoblasts. Histological and immunohistochemical staining and scanning electron microscopy observation indicated that the osteoblasts attached to and proliferated on the scaffolds. Extracellular matrices including collagen I and proteoglycan-like substrate were synthesized, while osteoid and bone-like tissue formed during the culture period. Furthermore, the cell/scaffold constructs had good biomineralization effect after 3 weeks in culture. © 2002 Elsevier Science Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterialsen_US
dc.relation.ispartofBiomaterialsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiocompatible Materials - Isolation & Purificationen_US
dc.subject.meshBiodegradation, Environmentalen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChitin - Analogs & Derivatives - Isolation & Purificationen_US
dc.subject.meshChitosanen_US
dc.subject.meshDurapatite - Isolation & Purificationen_US
dc.subject.meshGelatin - Isolation & Purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshMaterials Testingen_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshOsteoblasts - Cytology - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshTissue Engineeringen_US
dc.titlePreparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffoldsen_US
dc.typeArticleen_US
dc.identifier.emailLu, WW:wwlu@hku.hken_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0142-9612(02)00077-7en_US
dc.identifier.pmid12102194-
dc.identifier.scopuseid_2-s2.0-0035999776en_US
dc.identifier.hkuros71441-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035999776&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume23en_US
dc.identifier.issue15en_US
dc.identifier.spage3227en_US
dc.identifier.epage3234en_US
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridZhao, F=36040646100en_US
dc.identifier.scopusauthoridYin, Y=7403273865en_US
dc.identifier.scopusauthoridLu, WW=7404215221en_US
dc.identifier.scopusauthoridLeong, JC=35560782200en_US
dc.identifier.scopusauthoridZhang, W=36122097200en_US
dc.identifier.scopusauthoridZhang, J=8512129700en_US
dc.identifier.scopusauthoridZhang, M=8531407100en_US
dc.identifier.scopusauthoridYao, K=7403234424en_US

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