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Article: Apoptosis and p53 gene expression in male reproductive tissues of cadmium exposed rats
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TitleApoptosis and p53 gene expression in male reproductive tissues of cadmium exposed rats
 
AuthorsXu, G1 2
Zhou, G1
Jin, T1 2
Zhou, T1 2
Hammarström, S1
Bergh, A1
Nordberg, G
 
Issue Date1999
 
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0966-0844
 
CitationBiometals, 1999, v. 12 n. 2, p. 131-139 [How to Cite?]
DOI: http://dx.doi.org/10.1023/A:1009273711068
 
AbstractReverse transcription (RT) PCR technique was used to investigate the mechanism of apoptosis induced by Cd and the change of its related genes in testes and prostate of rats. Adult male rats were given a single (s.c.) injection of CdCl 2 0, 2.5, 5.0, 10 μmol/kg. 48 h and 72 h after administration of Cd, animals were sacrificed. The results indicated that Cd can induce apoptosis in testes via p53-independent pathway. No apoptosis occurred in prostate in any of the Cd-exposed groups. There was a clearly negative relationship in testes between p53 gene expression and Cd exposure and this dose-response relationship was observed both at 48 h and 72 h. There was a very small increase of this gene expression in the dorsolateral lobe of the prostate in Cd exposed groups. The other apoptosis related gene, bcl-x, was not detectable in either control or Cd-exposed group in testes and dorsal prostate. Although the MT-I gene was expressed in testes or dorsal prostate both in control and exposed groups, no overexpression of MT-I gene was found after administration of Cd. The expression of MT-I in the ventral prostate was not detected in the control group, but a weak expression was found after Cd exposure. Since p53 is a tumor suppressor gene which can inhibit tumorigenesis, the consequence of a Cd-induced decrease of p53 in testes may have a relation to the known risk of Cd tumorigenesis in this tissue.
 
ISSN0966-0844
2013 Impact Factor: 2.689
 
DOIhttp://dx.doi.org/10.1023/A:1009273711068
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorXu, G
 
dc.contributor.authorZhou, G
 
dc.contributor.authorJin, T
 
dc.contributor.authorZhou, T
 
dc.contributor.authorHammarström, S
 
dc.contributor.authorBergh, A
 
dc.contributor.authorNordberg, G
 
dc.date.accessioned2012-10-30T06:04:47Z
 
dc.date.available2012-10-30T06:04:47Z
 
dc.date.issued1999
 
dc.description.abstractReverse transcription (RT) PCR technique was used to investigate the mechanism of apoptosis induced by Cd and the change of its related genes in testes and prostate of rats. Adult male rats were given a single (s.c.) injection of CdCl 2 0, 2.5, 5.0, 10 μmol/kg. 48 h and 72 h after administration of Cd, animals were sacrificed. The results indicated that Cd can induce apoptosis in testes via p53-independent pathway. No apoptosis occurred in prostate in any of the Cd-exposed groups. There was a clearly negative relationship in testes between p53 gene expression and Cd exposure and this dose-response relationship was observed both at 48 h and 72 h. There was a very small increase of this gene expression in the dorsolateral lobe of the prostate in Cd exposed groups. The other apoptosis related gene, bcl-x, was not detectable in either control or Cd-exposed group in testes and dorsal prostate. Although the MT-I gene was expressed in testes or dorsal prostate both in control and exposed groups, no overexpression of MT-I gene was found after administration of Cd. The expression of MT-I in the ventral prostate was not detected in the control group, but a weak expression was found after Cd exposure. Since p53 is a tumor suppressor gene which can inhibit tumorigenesis, the consequence of a Cd-induced decrease of p53 in testes may have a relation to the known risk of Cd tumorigenesis in this tissue.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationBiometals, 1999, v. 12 n. 2, p. 131-139 [How to Cite?]
DOI: http://dx.doi.org/10.1023/A:1009273711068
 
dc.identifier.doihttp://dx.doi.org/10.1023/A:1009273711068
 
dc.identifier.epage139
 
dc.identifier.issn0966-0844
2013 Impact Factor: 2.689
 
dc.identifier.issue2
 
dc.identifier.pmid10406082
 
dc.identifier.scopuseid_2-s2.0-0033066279
 
dc.identifier.spage131
 
dc.identifier.urihttp://hdl.handle.net/10722/170018
 
dc.identifier.volume12
 
dc.languageeng
 
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0966-0844
 
dc.publisher.placeUnited States
 
dc.relation.ispartofBioMetals
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshApoptosis - Drug Effects
 
dc.subject.meshCadmium - Toxicity
 
dc.subject.meshGene Expression - Drug Effects
 
dc.subject.meshGenes, P53
 
dc.subject.meshMale
 
dc.subject.meshMetallothionein - Genetics
 
dc.subject.meshProstate - Drug Effects - Metabolism - Pathology
 
dc.subject.meshRna, Messenger - Analysis
 
dc.subject.meshRats
 
dc.subject.meshRats, Wistar
 
dc.subject.meshReproducibility Of Results
 
dc.subject.meshTestis - Drug Effects - Metabolism - Pathology
 
dc.titleApoptosis and p53 gene expression in male reproductive tissues of cadmium exposed rats
 
dc.typeArticle
 
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<contributor.author>Zhou, T</contributor.author>
<contributor.author>Hammarstr&#246;m, S</contributor.author>
<contributor.author>Bergh, A</contributor.author>
<contributor.author>Nordberg, G</contributor.author>
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Author Affiliations
  1. Umeå universitet
  2. null