Article: Apoptosis and p53 gene expression in male reproductive tissues of cadmium exposed rats

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TitleApoptosis and p53 gene expression in male reproductive tissues of cadmium exposed rats
AuthorsXu, G1 2
Zhou, G1
Jin, T1 2
Zhou, T1 2
Hammarström, S1
Bergh, A1
Nordberg, G
Issue Date1999
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0966-0844
CitationBiometals, 1999, v. 12 n. 2, p. 131-139 [How to Cite?]
DOI: http://dx.doi.org/10.1023/A:1009273711068
AbstractReverse transcription (RT) PCR technique was used to investigate the mechanism of apoptosis induced by Cd and the change of its related genes in testes and prostate of rats. Adult male rats were given a single (s.c.) injection of CdCl 2 0, 2.5, 5.0, 10 μmol/kg. 48 h and 72 h after administration of Cd, animals were sacrificed. The results indicated that Cd can induce apoptosis in testes via p53-independent pathway. No apoptosis occurred in prostate in any of the Cd-exposed groups. There was a clearly negative relationship in testes between p53 gene expression and Cd exposure and this dose-response relationship was observed both at 48 h and 72 h. There was a very small increase of this gene expression in the dorsolateral lobe of the prostate in Cd exposed groups. The other apoptosis related gene, bcl-x, was not detectable in either control or Cd-exposed group in testes and dorsal prostate. Although the MT-I gene was expressed in testes or dorsal prostate both in control and exposed groups, no overexpression of MT-I gene was found after administration of Cd. The expression of MT-I in the ventral prostate was not detected in the control group, but a weak expression was found after Cd exposure. Since p53 is a tumor suppressor gene which can inhibit tumorigenesis, the consequence of a Cd-induced decrease of p53 in testes may have a relation to the known risk of Cd tumorigenesis in this tissue.
ISSN0966-0844
2011 Impact Factor: 2.823
2011 SCImago Journal Rankings: 0.157
DOIhttp://dx.doi.org/10.1023/A:1009273711068
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorXu, G
dc.contributor.authorZhou, G
dc.contributor.authorJin, T
dc.contributor.authorZhou, T
dc.contributor.authorHammarström, S
dc.contributor.authorBergh, A
dc.contributor.authorNordberg, G
dc.date.accessioned2012-10-30T06:04:47Z
dc.date.available2012-10-30T06:04:47Z
dc.date.issued1999
dc.description.abstractReverse transcription (RT) PCR technique was used to investigate the mechanism of apoptosis induced by Cd and the change of its related genes in testes and prostate of rats. Adult male rats were given a single (s.c.) injection of CdCl 2 0, 2.5, 5.0, 10 μmol/kg. 48 h and 72 h after administration of Cd, animals were sacrificed. The results indicated that Cd can induce apoptosis in testes via p53-independent pathway. No apoptosis occurred in prostate in any of the Cd-exposed groups. There was a clearly negative relationship in testes between p53 gene expression and Cd exposure and this dose-response relationship was observed both at 48 h and 72 h. There was a very small increase of this gene expression in the dorsolateral lobe of the prostate in Cd exposed groups. The other apoptosis related gene, bcl-x, was not detectable in either control or Cd-exposed group in testes and dorsal prostate. Although the MT-I gene was expressed in testes or dorsal prostate both in control and exposed groups, no overexpression of MT-I gene was found after administration of Cd. The expression of MT-I in the ventral prostate was not detected in the control group, but a weak expression was found after Cd exposure. Since p53 is a tumor suppressor gene which can inhibit tumorigenesis, the consequence of a Cd-induced decrease of p53 in testes may have a relation to the known risk of Cd tumorigenesis in this tissue.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationBiometals, 1999, v. 12 n. 2, p. 131-139 [How to Cite?]
DOI: http://dx.doi.org/10.1023/A:1009273711068
dc.identifier.doihttp://dx.doi.org/10.1023/A:1009273711068
dc.identifier.epage139
dc.identifier.issn0966-0844
2011 Impact Factor: 2.823
2011 SCImago Journal Rankings: 0.157
dc.identifier.issue2
dc.identifier.pmid10406082
dc.identifier.scopuseid_2-s2.0-0033066279
dc.identifier.spage131
dc.identifier.urihttp://hdl.handle.net/10722/170018
dc.identifier.volume12
dc.languageeng
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0966-0844
dc.publisher.placeUnited States
dc.relation.ispartofBioMetals
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshApoptosis - Drug Effects
dc.subject.meshCadmium - Toxicity
dc.subject.meshGene Expression - Drug Effects
dc.subject.meshGenes, P53
dc.subject.meshMale
dc.subject.meshMetallothionein - Genetics
dc.subject.meshProstate - Drug Effects - Metabolism - Pathology
dc.subject.meshRna, Messenger - Analysis
dc.subject.meshRats
dc.subject.meshRats, Wistar
dc.subject.meshReproducibility Of Results
dc.subject.meshTestis - Drug Effects - Metabolism - Pathology
dc.titleApoptosis and p53 gene expression in male reproductive tissues of cadmium exposed rats
dc.typeArticle
Author Affiliations
  1. Umeå universitet
  2. null