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Article: Cadmium-induced apoptosis and changes in expression of p53, c-jun and MT-I genes in testes and ventral prostate of rats
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TitleCadmium-induced apoptosis and changes in expression of p53, c-jun and MT-I genes in testes and ventral prostate of rats
 
AuthorsZhou, T1 2
Zhou, G1
Song, W1 2
Eguchi, N1
Lu, W1
Lundin, E1
Jin, T1 2
Nordberg, G1
 
Issue Date1999
 
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxicol
 
CitationToxicology, 1999, v. 142 n. 1, p. 1-13 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0300-483X(99)00115-8
 
AbstractApoptosis and a change in the expression of p53, c-jun and MT-I genes occurred in rats exposed to cadmium in a way known to cause carcinogenesis in testes and ventral prostate. In situ end labelling (ISEL), DNA electrophoresis, and RT-PCR methods were used in present study. Adult male Wistar rats were given a single (s.c.) injection of 0, 5, 10, or 20 μmol/kg CdCl2. Then 12, 48 or 96 h after administration of cadmium, animals were sacrificed. It was observed that cadmium markedly induced apoptosis in the testes at the dose of 5 μmol/kg while 10 and 20 μmol/kg cadmium caused more necrosis than apoptosis. Apoptosis in the ventral prostate was markedly induced by all the doses of cadmium and there was an obvious time- and dose-dependent relationship between apoptotic index (AI) and cadmium treatment. Far fewer apoptotic cells appeared in liver, compared to the testes and ventral prostate. p53 mRNA expression was clearly enhanced in the ventral prostate but clearly suppressed in the testes by cadmium exposure, and the time- and dose-effect was very clear. The expression level of p53 in the liver was not affected by cadmium treatment. Cadmium-induced overexpression of c-jun gene appeared at 12 h in the liver, but not until 96 h in the testes and ventral prostate. Although the MT-I gene was found to be expressed in all tissues, marked induction by cadmium of the expression of MT-I gene was only observed in the liver. These results indicate: (1) that apoptosis is an early mechanism of acute tissue damage by cadmium in the testes and ventral prostate; (2) that p53 and c-jun genes may be involved in cadmium-induced cytotoxicity (apoptosis) and related carcinogenicity in male reproductive tissues; and (3) that the enhanced expression of MT-I in the liver could protect this organ from cadmium-induced cytotoxicity (apoptosis) and carcinogenicity. Copyright (C) 1999 Elsevier Science Ireland Ltd.
 
ISSN0300-483X
2013 Impact Factor: 3.745
2013 SCImago Journal Rankings: 1.196
 
DOIhttp://dx.doi.org/10.1016/S0300-483X(99)00115-8
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhou, T
 
dc.contributor.authorZhou, G
 
dc.contributor.authorSong, W
 
dc.contributor.authorEguchi, N
 
dc.contributor.authorLu, W
 
dc.contributor.authorLundin, E
 
dc.contributor.authorJin, T
 
dc.contributor.authorNordberg, G
 
dc.date.accessioned2012-10-30T06:04:46Z
 
dc.date.available2012-10-30T06:04:46Z
 
dc.date.issued1999
 
dc.description.abstractApoptosis and a change in the expression of p53, c-jun and MT-I genes occurred in rats exposed to cadmium in a way known to cause carcinogenesis in testes and ventral prostate. In situ end labelling (ISEL), DNA electrophoresis, and RT-PCR methods were used in present study. Adult male Wistar rats were given a single (s.c.) injection of 0, 5, 10, or 20 μmol/kg CdCl2. Then 12, 48 or 96 h after administration of cadmium, animals were sacrificed. It was observed that cadmium markedly induced apoptosis in the testes at the dose of 5 μmol/kg while 10 and 20 μmol/kg cadmium caused more necrosis than apoptosis. Apoptosis in the ventral prostate was markedly induced by all the doses of cadmium and there was an obvious time- and dose-dependent relationship between apoptotic index (AI) and cadmium treatment. Far fewer apoptotic cells appeared in liver, compared to the testes and ventral prostate. p53 mRNA expression was clearly enhanced in the ventral prostate but clearly suppressed in the testes by cadmium exposure, and the time- and dose-effect was very clear. The expression level of p53 in the liver was not affected by cadmium treatment. Cadmium-induced overexpression of c-jun gene appeared at 12 h in the liver, but not until 96 h in the testes and ventral prostate. Although the MT-I gene was found to be expressed in all tissues, marked induction by cadmium of the expression of MT-I gene was only observed in the liver. These results indicate: (1) that apoptosis is an early mechanism of acute tissue damage by cadmium in the testes and ventral prostate; (2) that p53 and c-jun genes may be involved in cadmium-induced cytotoxicity (apoptosis) and related carcinogenicity in male reproductive tissues; and (3) that the enhanced expression of MT-I in the liver could protect this organ from cadmium-induced cytotoxicity (apoptosis) and carcinogenicity. Copyright (C) 1999 Elsevier Science Ireland Ltd.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationToxicology, 1999, v. 142 n. 1, p. 1-13 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0300-483X(99)00115-8
 
dc.identifier.doihttp://dx.doi.org/10.1016/S0300-483X(99)00115-8
 
dc.identifier.epage13
 
dc.identifier.issn0300-483X
2013 Impact Factor: 3.745
2013 SCImago Journal Rankings: 1.196
 
dc.identifier.issue1
 
dc.identifier.pmid10647914
 
dc.identifier.scopuseid_2-s2.0-0032704482
 
dc.identifier.spage1
 
dc.identifier.urihttp://hdl.handle.net/10722/170016
 
dc.identifier.volume142
 
dc.languageeng
 
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxicol
 
dc.publisher.placeIreland
 
dc.relation.ispartofToxicology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshApoptosis - Drug Effects
 
dc.subject.meshCadmium - Toxicity
 
dc.subject.meshElectrophoresis
 
dc.subject.meshGene Expression Regulation - Drug Effects
 
dc.subject.meshGenes, Jun - Drug Effects
 
dc.subject.meshGenes, P53 - Drug Effects
 
dc.subject.meshLiver - Drug Effects - Metabolism - Physiology
 
dc.subject.meshMale
 
dc.subject.meshMetallothionein - Genetics
 
dc.subject.meshProstate - Drug Effects - Metabolism - Pathology
 
dc.subject.meshRna, Messenger - Metabolism
 
dc.subject.meshRats
 
dc.subject.meshRats, Wistar
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.subject.meshTestis - Drug Effects - Metabolism - Pathology
 
dc.subject.meshTranscription, Genetic - Drug Effects
 
dc.titleCadmium-induced apoptosis and changes in expression of p53, c-jun and MT-I genes in testes and ventral prostate of rats
 
dc.typeArticle
 
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Author Affiliations
  1. Umeå universitet
  2. null