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Article: ZIP2 and ZIP4 mediate age-related zinc fluxes across the retinal pigment epithelium
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TitleZIP2 and ZIP4 mediate age-related zinc fluxes across the retinal pigment epithelium
 
AuthorsLeung, KW1
Gvritishvili, A1
Liu, Y1
TombranTink, J1
 
KeywordsAge-related macular degeneration
Aging
Retina
RPE cells
Zinc transport
Zinc transporters
 
Issue Date2012
 
CitationJournal Of Molecular Neuroscience, 2012, v. 46 n. 2, p. 122-137 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s12031-011-9536-0
 
AbstractDecreases in systemic and cellular levels of zinc (Zn 2+) during normal aging correlate with several age-related pathologies including age-related macular degeneration. Zn 2+ homeostasis in tissues is not only dependent on dietary intake but also on optimal expression and function of its influx (ZIP) and efflux (ZnT) transporters. We recently showed that many of the Zn 2+ transporters are expressed by the retinal pigment epithelial (RPE) cells. In this study, we present evidence that RPE cells contain less endogenous Zn 2+ with increased aging and transport this ion vectorially with greater transport from the basal to apical direction. Expression of two Zn 2+ influx transporters, ZIP2 and ZIP4, is reduced as a function of RPE age. Gene silencing of ZIP2 and ZIP4 in RPE cells from young donors or their overexpression in cells from older donors confirms that these two transporters are essential in controlling Zn 2+ influx and sequestration in RPE cells. Both transporters are distributed on the basal surface of the RPE where they are likely to control Zn 2+ homeostasis in the outer retina. © Springer Science+Business Media, LLC 2011.
 
ISSN0895-8696
2013 Impact Factor: 2.757
2013 SCImago Journal Rankings: 1.242
 
DOIhttp://dx.doi.org/10.1007/s12031-011-9536-0
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLeung, KW
 
dc.contributor.authorGvritishvili, A
 
dc.contributor.authorLiu, Y
 
dc.contributor.authorTombranTink, J
 
dc.date.accessioned2012-10-25T04:57:11Z
 
dc.date.available2012-10-25T04:57:11Z
 
dc.date.issued2012
 
dc.description.abstractDecreases in systemic and cellular levels of zinc (Zn 2+) during normal aging correlate with several age-related pathologies including age-related macular degeneration. Zn 2+ homeostasis in tissues is not only dependent on dietary intake but also on optimal expression and function of its influx (ZIP) and efflux (ZnT) transporters. We recently showed that many of the Zn 2+ transporters are expressed by the retinal pigment epithelial (RPE) cells. In this study, we present evidence that RPE cells contain less endogenous Zn 2+ with increased aging and transport this ion vectorially with greater transport from the basal to apical direction. Expression of two Zn 2+ influx transporters, ZIP2 and ZIP4, is reduced as a function of RPE age. Gene silencing of ZIP2 and ZIP4 in RPE cells from young donors or their overexpression in cells from older donors confirms that these two transporters are essential in controlling Zn 2+ influx and sequestration in RPE cells. Both transporters are distributed on the basal surface of the RPE where they are likely to control Zn 2+ homeostasis in the outer retina. © Springer Science+Business Media, LLC 2011.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Molecular Neuroscience, 2012, v. 46 n. 2, p. 122-137 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s12031-011-9536-0
 
dc.identifier.citeulike9363874
 
dc.identifier.doihttp://dx.doi.org/10.1007/s12031-011-9536-0
 
dc.identifier.epage137
 
dc.identifier.issn0895-8696
2013 Impact Factor: 2.757
2013 SCImago Journal Rankings: 1.242
 
dc.identifier.issue2
 
dc.identifier.pmid21603979
 
dc.identifier.scopuseid_2-s2.0-84863385420
 
dc.identifier.spage122
 
dc.identifier.urihttp://hdl.handle.net/10722/169868
 
dc.identifier.volume46
 
dc.languageeng
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Molecular Neuroscience
 
dc.relation.referencesReferences in Scopus
 
dc.subjectAge-related macular degeneration
 
dc.subjectAging
 
dc.subjectRetina
 
dc.subjectRPE cells
 
dc.subjectZinc transport
 
dc.subjectZinc transporters
 
dc.titleZIP2 and ZIP4 mediate age-related zinc fluxes across the retinal pigment epithelium
 
dc.typeArticle
 
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Author Affiliations
  1. Penn State College of Medicine