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Article: MiR-200b regulates cell migration via Zeb family during mouse palate development

TitleMiR-200b regulates cell migration via Zeb family during mouse palate development
Authors
Issue Date2012
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
Citation
Histochemistry And Cell Biology, 2012, v. 137 n. 4, p. 459-470 How to Cite?
AbstractPalate development requires coordinating proper cellular and molecular events in palatogenesis, including the epithelial-mesenchymal transition (EMT), apoptosis, cell proliferation, and cell migration. Zeb1 and Zeb2 regulate epithelial cadherin (E-cadherin) and EMT during organogenesis. While microRNA 200b (miR-200b) is known to be a negative regulator of Zeb1 and Zeb2 in cancer progression, its regulatory eVects on Zeb1 and Zeb2 in palatogenesis have not yet been clariWed. The aim of this study is to investigate the relationship between the regulators of palatal development, speciWcally, miR-200b and the Zeb family. Expression of both Zeb1 and Zeb2 was detected in the mesenchyme of the mouse palate, while miR-200b was expressed in the medial edge epithelium. After contact with the palatal shelves, miR-200b was expressed in the palatal epithelial lining and epithelial island around the fusion region but not in the palatal mesenchyme. The function of miR-200b was examined by overexpression via a lentiviral vector in the palatal shelves. Ectopic expression of miR-200b resulted in suppression of the Zeb family, upregulation of E-cadherin, and changes in cell migration and palatal fusion. These results suggest that miR-200b plays crucial roles in cell migration and palatal fusion by regulating Zeb1 and Zeb2 as a noncoding RNA during palate development.© 2012 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/169593
ISSN
2015 Impact Factor: 2.78
2015 SCImago Journal Rankings: 1.287
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShin, JOen_US
dc.contributor.authorNakagawa, Een_US
dc.contributor.authorKim, EJen_US
dc.contributor.authorCho, KWen_US
dc.contributor.authorLee, JMen_US
dc.contributor.authorCho, SWen_US
dc.contributor.authorJung, HSen_US
dc.date.accessioned2012-10-25T04:53:15Z-
dc.date.available2012-10-25T04:53:15Z-
dc.date.issued2012en_US
dc.identifier.citationHistochemistry And Cell Biology, 2012, v. 137 n. 4, p. 459-470en_US
dc.identifier.issn0948-6143en_US
dc.identifier.urihttp://hdl.handle.net/10722/169593-
dc.description.abstractPalate development requires coordinating proper cellular and molecular events in palatogenesis, including the epithelial-mesenchymal transition (EMT), apoptosis, cell proliferation, and cell migration. Zeb1 and Zeb2 regulate epithelial cadherin (E-cadherin) and EMT during organogenesis. While microRNA 200b (miR-200b) is known to be a negative regulator of Zeb1 and Zeb2 in cancer progression, its regulatory eVects on Zeb1 and Zeb2 in palatogenesis have not yet been clariWed. The aim of this study is to investigate the relationship between the regulators of palatal development, speciWcally, miR-200b and the Zeb family. Expression of both Zeb1 and Zeb2 was detected in the mesenchyme of the mouse palate, while miR-200b was expressed in the medial edge epithelium. After contact with the palatal shelves, miR-200b was expressed in the palatal epithelial lining and epithelial island around the fusion region but not in the palatal mesenchyme. The function of miR-200b was examined by overexpression via a lentiviral vector in the palatal shelves. Ectopic expression of miR-200b resulted in suppression of the Zeb family, upregulation of E-cadherin, and changes in cell migration and palatal fusion. These results suggest that miR-200b plays crucial roles in cell migration and palatal fusion by regulating Zeb1 and Zeb2 as a noncoding RNA during palate development.© 2012 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htmen_US
dc.relation.ispartofHistochemistry and Cell Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCadherins - Genetics - Metabolismen_US
dc.subject.meshCell Movementen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEpithelial-Mesenchymal Transitionen_US
dc.subject.meshHomeodomain Proteins - Genetics - Metabolismen_US
dc.subject.meshKruppel-Like Transcription Factors - Genetics - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Icren_US
dc.subject.meshMicrornas - Genetics - Metabolismen_US
dc.subject.meshPalate - Embryology - Metabolismen_US
dc.subject.meshRepressor Proteins - Genetics - Metabolismen_US
dc.titleMiR-200b regulates cell migration via Zeb family during mouse palate developmenten_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00418-012-0915-6en_US
dc.identifier.pmid22261924-
dc.identifier.scopuseid_2-s2.0-84862836550en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862836550&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume137en_US
dc.identifier.issue4en_US
dc.identifier.spage459en_US
dc.identifier.epage470en_US
dc.identifier.isiWOS:000302324700002-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridShin, JO=37361704500en_US
dc.identifier.scopusauthoridNakagawa, E=52364474400en_US
dc.identifier.scopusauthoridKim, EJ=36064163200en_US
dc.identifier.scopusauthoridCho, KW=7403956665en_US
dc.identifier.scopusauthoridLee, JM=41361401200en_US
dc.identifier.scopusauthoridCho, SW=32967447200en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.citeulike10262260-

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