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Article: The novel function of Oct3/4 in mouse tooth development

TitleThe novel function of Oct3/4 in mouse tooth development
Authors
Issue Date2012
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
Citation
Histochemistry And Cell Biology, 2012, v. 137 n. 3, p. 367-376 How to Cite?
AbstractOctamer-binding factor 3/4 (Oct3/4) is one of the key regulators maintaining the pluripotency and self-renewal in embryonic stem cells and is involved in the developmental events. However, the functional significance of Oct3/4 remains to be clarified during tooth morphogenesis. This study aimed to examine the functional role of Oct3/4 in mouse. During tooth morphogenesis (E11-E16.5), Oct3/4-positive cells, detected by nuclear immunoreaction, increased in number, and subsequently, their immunoreaction shifted from the nucleus to the cytoplasm at the stage of cell differentiation (E18.5). Quantitative real-time PCR clearly demonstrated the relationship between isoforms of Oct3/4 and the in vivo cellular localization of Oct3/4, suggesting that the Oct3/4 expressed in nucleus was Oct3/4A, whereas that expressed in the cytoplasm was Oct3/4B. RNAi knockdown of Oct3/4 induced apoptosis and arrested tooth morphogenesis. Our results suggest that (1) the increased number of Oct3/4-positive cells with nuclear immunoreaction correlate with active cell proliferation during tooth morphogenesis and (2) the shift of Oct3/4 from the nucleus to the cytoplasm plays a crucial role in cell differentiation. © 2011 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/169590
ISSN
2015 Impact Factor: 2.78
2015 SCImago Journal Rankings: 1.287
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNakagawa, Een_US
dc.contributor.authorZhang, Len_US
dc.contributor.authorKim, EJen_US
dc.contributor.authorShin, JOen_US
dc.contributor.authorCho, SWen_US
dc.contributor.authorOhshima, Hen_US
dc.contributor.authorJung, HSen_US
dc.date.accessioned2012-10-25T04:53:13Z-
dc.date.available2012-10-25T04:53:13Z-
dc.date.issued2012en_US
dc.identifier.citationHistochemistry And Cell Biology, 2012, v. 137 n. 3, p. 367-376en_US
dc.identifier.issn0948-6143en_US
dc.identifier.urihttp://hdl.handle.net/10722/169590-
dc.description.abstractOctamer-binding factor 3/4 (Oct3/4) is one of the key regulators maintaining the pluripotency and self-renewal in embryonic stem cells and is involved in the developmental events. However, the functional significance of Oct3/4 remains to be clarified during tooth morphogenesis. This study aimed to examine the functional role of Oct3/4 in mouse. During tooth morphogenesis (E11-E16.5), Oct3/4-positive cells, detected by nuclear immunoreaction, increased in number, and subsequently, their immunoreaction shifted from the nucleus to the cytoplasm at the stage of cell differentiation (E18.5). Quantitative real-time PCR clearly demonstrated the relationship between isoforms of Oct3/4 and the in vivo cellular localization of Oct3/4, suggesting that the Oct3/4 expressed in nucleus was Oct3/4A, whereas that expressed in the cytoplasm was Oct3/4B. RNAi knockdown of Oct3/4 induced apoptosis and arrested tooth morphogenesis. Our results suggest that (1) the increased number of Oct3/4-positive cells with nuclear immunoreaction correlate with active cell proliferation during tooth morphogenesis and (2) the shift of Oct3/4 from the nucleus to the cytoplasm plays a crucial role in cell differentiation. © 2011 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htmen_US
dc.relation.ispartofHistochemistry and Cell Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis - Physiologyen_US
dc.subject.meshCell Differentiation - Physiologyen_US
dc.subject.meshCell Nucleus - Metabolismen_US
dc.subject.meshCytoplasm - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation, Developmental - Physiologyen_US
dc.subject.meshGene Knockdown Techniquesen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Icren_US
dc.subject.meshOctamer Transcription Factor-3 - Genetics - Metabolismen_US
dc.subject.meshOdontogenesis - Physiologyen_US
dc.subject.meshOrgan Culture Techniquesen_US
dc.subject.meshPregnancyen_US
dc.subject.meshTooth - Cytology - Embryology - Physiologyen_US
dc.titleThe novel function of Oct3/4 in mouse tooth developmenten_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00418-011-0895-yen_US
dc.identifier.pmid22159899-
dc.identifier.scopuseid_2-s2.0-84857638648en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84857638648&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume137en_US
dc.identifier.issue3en_US
dc.identifier.spage367en_US
dc.identifier.epage376en_US
dc.identifier.isiWOS:000300326100009-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridNakagawa, E=52364474400en_US
dc.identifier.scopusauthoridZhang, L=54418382600en_US
dc.identifier.scopusauthoridKim, EJ=36064163200en_US
dc.identifier.scopusauthoridShin, JO=37361704500en_US
dc.identifier.scopusauthoridCho, SW=32967447200en_US
dc.identifier.scopusauthoridOhshima, H=7202879991en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.citeulike10128478-

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