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Article: Shh signaling is essential for rugae morphogenesis in mice

TitleShh signaling is essential for rugae morphogenesis in mice
Authors
Issue Date2011
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
Citation
Histochemistry And Cell Biology, 2011, v. 136 n. 6, p. 663-675 How to Cite?
Abstract
Palatal ridges, or rugae palatinae, are corrugated structures observed in the hard palate region. They are found in most mammalian species, but their number and arrangement are species-specific. Nine palatal rugae are found in the mouse secondary palate. Previous studies have shown that epithelial Shh signaling in the palatal ridge plays an important role during rugae development. Moreover, Wnt family members, including LEF1, play a functional role in orofacial morphogenesis. To explore the function of Shh during rugae development, we utilized the maternal transfer of 5E1 (anti-Shh antibody) to mouse embryos. 5E1 induced abnormal rugae patterning characterized by a spotted shape of palatal ridge rather than a stripe. The expression patterns of Shh and Shh-related genes, Sostdc1, Lef1 and Ptch1, were disrupted following 5E1 injection. Moreover, rugae-specific cell proliferation and inter-rugae-specific apoptosis were affected by inhibition of Shh signaling. We hypothesize that the altered gene expression patterns and the change in molecular events caused by the inhibition of Shh signaling may have induced abnormal rugae patterning. Furthermore, we propose a reaction-diffusion model generated by Wnt, Shh and Sostdc1 signaling. In this study, we show that Sostdc1, a secreted inhibitor of the Wnt pathway, is a downstream target of Shh and hypothesize that the interaction of Wnt, Shh and Sostdc1 is a pivotal mechanism controlling the spatial patterning of palatal rugae. © 2011 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/169588
ISSN
2013 Impact Factor: 2.927
ISI Accession Number ID
References

 

Author Affiliations
  1. Yonsei University
  2. Osaka University
DC FieldValueLanguage
dc.contributor.authorLee, JMen_US
dc.contributor.authorMiyazawa, Sen_US
dc.contributor.authorShin, JOen_US
dc.contributor.authorKwon, HJen_US
dc.contributor.authorKang, DWen_US
dc.contributor.authorChoi, BJen_US
dc.contributor.authorLee, JHen_US
dc.contributor.authorKondo, Sen_US
dc.contributor.authorCho, SWen_US
dc.contributor.authorJung, HSen_US
dc.date.accessioned2012-10-25T04:53:12Z-
dc.date.available2012-10-25T04:53:12Z-
dc.date.issued2011en_US
dc.identifier.citationHistochemistry And Cell Biology, 2011, v. 136 n. 6, p. 663-675en_US
dc.identifier.issn0948-6143en_US
dc.identifier.urihttp://hdl.handle.net/10722/169588-
dc.description.abstractPalatal ridges, or rugae palatinae, are corrugated structures observed in the hard palate region. They are found in most mammalian species, but their number and arrangement are species-specific. Nine palatal rugae are found in the mouse secondary palate. Previous studies have shown that epithelial Shh signaling in the palatal ridge plays an important role during rugae development. Moreover, Wnt family members, including LEF1, play a functional role in orofacial morphogenesis. To explore the function of Shh during rugae development, we utilized the maternal transfer of 5E1 (anti-Shh antibody) to mouse embryos. 5E1 induced abnormal rugae patterning characterized by a spotted shape of palatal ridge rather than a stripe. The expression patterns of Shh and Shh-related genes, Sostdc1, Lef1 and Ptch1, were disrupted following 5E1 injection. Moreover, rugae-specific cell proliferation and inter-rugae-specific apoptosis were affected by inhibition of Shh signaling. We hypothesize that the altered gene expression patterns and the change in molecular events caused by the inhibition of Shh signaling may have induced abnormal rugae patterning. Furthermore, we propose a reaction-diffusion model generated by Wnt, Shh and Sostdc1 signaling. In this study, we show that Sostdc1, a secreted inhibitor of the Wnt pathway, is a downstream target of Shh and hypothesize that the interaction of Wnt, Shh and Sostdc1 is a pivotal mechanism controlling the spatial patterning of palatal rugae. © 2011 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htmen_US
dc.relation.ispartofHistochemistry and Cell Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Pharmacologyen_US
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshCell Proliferation - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshComputer Simulationen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshHedgehog Proteins - Antagonists & Inhibitors - Genetics - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMicroarray Analysisen_US
dc.subject.meshPalate - Growth & Developmenten_US
dc.subject.meshReal-Time Polymerase Chain Reactionen_US
dc.subject.meshSignal Transductionen_US
dc.titleShh signaling is essential for rugae morphogenesis in miceen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00418-011-0870-7en_US
dc.identifier.pmid22038040en_US
dc.identifier.scopuseid_2-s2.0-84855247292en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84855247292&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume136en_US
dc.identifier.issue6en_US
dc.identifier.spage663en_US
dc.identifier.epage675en_US
dc.identifier.isiWOS:000297129100004-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridLee, JM=41361401200en_US
dc.identifier.scopusauthoridMiyazawa, S=36675627400en_US
dc.identifier.scopusauthoridShin, JO=37361704500en_US
dc.identifier.scopusauthoridKwon, HJ=18836582500en_US
dc.identifier.scopusauthoridKang, DW=53877581400en_US
dc.identifier.scopusauthoridChoi, BJ=37260950700en_US
dc.identifier.scopusauthoridLee, JH=26661939800en_US
dc.identifier.scopusauthoridKondo, S=7403377131en_US
dc.identifier.scopusauthoridCho, SW=32967447200en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.citeulike9979285-

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