Article: Shh signaling is essential for rugae morphogenesis in mice

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TitleShh signaling is essential for rugae morphogenesis in mice
AuthorsLee, JM1
Miyazawa, S2
Shin, JO1
Kwon, HJ1
Kang, DW1
Choi, BJ1
Lee, JH1
Kondo, S2
Cho, SW1
Jung, HS1
Issue Date2011
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
CitationHistochemistry And Cell Biology, 2011, v. 136 n. 6, p. 663-675 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00418-011-0870-7
AbstractPalatal ridges, or rugae palatinae, are corrugated structures observed in the hard palate region. They are found in most mammalian species, but their number and arrangement are species-specific. Nine palatal rugae are found in the mouse secondary palate. Previous studies have shown that epithelial Shh signaling in the palatal ridge plays an important role during rugae development. Moreover, Wnt family members, including LEF1, play a functional role in orofacial morphogenesis. To explore the function of Shh during rugae development, we utilized the maternal transfer of 5E1 (anti-Shh antibody) to mouse embryos. 5E1 induced abnormal rugae patterning characterized by a spotted shape of palatal ridge rather than a stripe. The expression patterns of Shh and Shh-related genes, Sostdc1, Lef1 and Ptch1, were disrupted following 5E1 injection. Moreover, rugae-specific cell proliferation and inter-rugae-specific apoptosis were affected by inhibition of Shh signaling. We hypothesize that the altered gene expression patterns and the change in molecular events caused by the inhibition of Shh signaling may have induced abnormal rugae patterning. Furthermore, we propose a reaction-diffusion model generated by Wnt, Shh and Sostdc1 signaling. In this study, we show that Sostdc1, a secreted inhibitor of the Wnt pathway, is a downstream target of Shh and hypothesize that the interaction of Wnt, Shh and Sostdc1 is a pivotal mechanism controlling the spatial patterning of palatal rugae. © 2011 Springer-Verlag.
ISSN0948-6143
2011 Impact Factor: 2.588
2011 SCImago Journal Rankings: 0.474
DOIhttp://dx.doi.org/10.1007/s00418-011-0870-7
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLee, JM
dc.contributor.authorMiyazawa, S
dc.contributor.authorShin, JO
dc.contributor.authorKwon, HJ
dc.contributor.authorKang, DW
dc.contributor.authorChoi, BJ
dc.contributor.authorLee, JH
dc.contributor.authorKondo, S
dc.contributor.authorCho, SW
dc.contributor.authorJung, HS
dc.date.accessioned2012-10-25T04:53:12Z
dc.date.available2012-10-25T04:53:12Z
dc.date.issued2011
dc.description.abstractPalatal ridges, or rugae palatinae, are corrugated structures observed in the hard palate region. They are found in most mammalian species, but their number and arrangement are species-specific. Nine palatal rugae are found in the mouse secondary palate. Previous studies have shown that epithelial Shh signaling in the palatal ridge plays an important role during rugae development. Moreover, Wnt family members, including LEF1, play a functional role in orofacial morphogenesis. To explore the function of Shh during rugae development, we utilized the maternal transfer of 5E1 (anti-Shh antibody) to mouse embryos. 5E1 induced abnormal rugae patterning characterized by a spotted shape of palatal ridge rather than a stripe. The expression patterns of Shh and Shh-related genes, Sostdc1, Lef1 and Ptch1, were disrupted following 5E1 injection. Moreover, rugae-specific cell proliferation and inter-rugae-specific apoptosis were affected by inhibition of Shh signaling. We hypothesize that the altered gene expression patterns and the change in molecular events caused by the inhibition of Shh signaling may have induced abnormal rugae patterning. Furthermore, we propose a reaction-diffusion model generated by Wnt, Shh and Sostdc1 signaling. In this study, we show that Sostdc1, a secreted inhibitor of the Wnt pathway, is a downstream target of Shh and hypothesize that the interaction of Wnt, Shh and Sostdc1 is a pivotal mechanism controlling the spatial patterning of palatal rugae. © 2011 Springer-Verlag.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationHistochemistry And Cell Biology, 2011, v. 136 n. 6, p. 663-675 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00418-011-0870-7
dc.identifier.citeulike9979285
dc.identifier.doihttp://dx.doi.org/10.1007/s00418-011-0870-7
dc.identifier.epage675
dc.identifier.issn0948-6143
2011 Impact Factor: 2.588
2011 SCImago Journal Rankings: 0.474
dc.identifier.issue6
dc.identifier.pmid22038040
dc.identifier.scopuseid_2-s2.0-84855247292
dc.identifier.spage663
dc.identifier.urihttp://hdl.handle.net/10722/169588
dc.identifier.volume136
dc.languageeng
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
dc.publisher.placeGermany
dc.relation.ispartofHistochemistry and Cell Biology
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshAntibodies, Monoclonal - Pharmacology
dc.subject.meshApoptosis - Drug Effects
dc.subject.meshCell Proliferation - Drug Effects
dc.subject.meshCells, Cultured
dc.subject.meshComputer Simulation
dc.subject.meshGene Expression Regulation - Drug Effects
dc.subject.meshHedgehog Proteins - Antagonists & Inhibitors - Genetics - Metabolism
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshIn Situ Hybridization
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMicroarray Analysis
dc.subject.meshPalate - Growth & Development
dc.subject.meshReal-Time Polymerase Chain Reaction
dc.subject.meshSignal Transduction
dc.titleShh signaling is essential for rugae morphogenesis in mice
dc.typeArticle
Author Affiliations
  1. Yonsei University
  2. Osaka University