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Article: Shh signaling is essential for rugae morphogenesis in mice
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TitleShh signaling is essential for rugae morphogenesis in mice
 
AuthorsLee, JM1
Miyazawa, S2
Shin, JO1
Kwon, HJ1
Kang, DW1
Choi, BJ1
Lee, JH1
Kondo, S2
Cho, SW1
Jung, HS1
 
Issue Date2011
 
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
 
CitationHistochemistry And Cell Biology, 2011, v. 136 n. 6, p. 663-675 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00418-011-0870-7
 
AbstractPalatal ridges, or rugae palatinae, are corrugated structures observed in the hard palate region. They are found in most mammalian species, but their number and arrangement are species-specific. Nine palatal rugae are found in the mouse secondary palate. Previous studies have shown that epithelial Shh signaling in the palatal ridge plays an important role during rugae development. Moreover, Wnt family members, including LEF1, play a functional role in orofacial morphogenesis. To explore the function of Shh during rugae development, we utilized the maternal transfer of 5E1 (anti-Shh antibody) to mouse embryos. 5E1 induced abnormal rugae patterning characterized by a spotted shape of palatal ridge rather than a stripe. The expression patterns of Shh and Shh-related genes, Sostdc1, Lef1 and Ptch1, were disrupted following 5E1 injection. Moreover, rugae-specific cell proliferation and inter-rugae-specific apoptosis were affected by inhibition of Shh signaling. We hypothesize that the altered gene expression patterns and the change in molecular events caused by the inhibition of Shh signaling may have induced abnormal rugae patterning. Furthermore, we propose a reaction-diffusion model generated by Wnt, Shh and Sostdc1 signaling. In this study, we show that Sostdc1, a secreted inhibitor of the Wnt pathway, is a downstream target of Shh and hypothesize that the interaction of Wnt, Shh and Sostdc1 is a pivotal mechanism controlling the spatial patterning of palatal rugae. © 2011 Springer-Verlag.
 
ISSN0948-6143
2013 Impact Factor: 2.927
 
DOIhttp://dx.doi.org/10.1007/s00418-011-0870-7
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLee, JM
 
dc.contributor.authorMiyazawa, S
 
dc.contributor.authorShin, JO
 
dc.contributor.authorKwon, HJ
 
dc.contributor.authorKang, DW
 
dc.contributor.authorChoi, BJ
 
dc.contributor.authorLee, JH
 
dc.contributor.authorKondo, S
 
dc.contributor.authorCho, SW
 
dc.contributor.authorJung, HS
 
dc.date.accessioned2012-10-25T04:53:12Z
 
dc.date.available2012-10-25T04:53:12Z
 
dc.date.issued2011
 
dc.description.abstractPalatal ridges, or rugae palatinae, are corrugated structures observed in the hard palate region. They are found in most mammalian species, but their number and arrangement are species-specific. Nine palatal rugae are found in the mouse secondary palate. Previous studies have shown that epithelial Shh signaling in the palatal ridge plays an important role during rugae development. Moreover, Wnt family members, including LEF1, play a functional role in orofacial morphogenesis. To explore the function of Shh during rugae development, we utilized the maternal transfer of 5E1 (anti-Shh antibody) to mouse embryos. 5E1 induced abnormal rugae patterning characterized by a spotted shape of palatal ridge rather than a stripe. The expression patterns of Shh and Shh-related genes, Sostdc1, Lef1 and Ptch1, were disrupted following 5E1 injection. Moreover, rugae-specific cell proliferation and inter-rugae-specific apoptosis were affected by inhibition of Shh signaling. We hypothesize that the altered gene expression patterns and the change in molecular events caused by the inhibition of Shh signaling may have induced abnormal rugae patterning. Furthermore, we propose a reaction-diffusion model generated by Wnt, Shh and Sostdc1 signaling. In this study, we show that Sostdc1, a secreted inhibitor of the Wnt pathway, is a downstream target of Shh and hypothesize that the interaction of Wnt, Shh and Sostdc1 is a pivotal mechanism controlling the spatial patterning of palatal rugae. © 2011 Springer-Verlag.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationHistochemistry And Cell Biology, 2011, v. 136 n. 6, p. 663-675 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00418-011-0870-7
 
dc.identifier.citeulike9979285
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00418-011-0870-7
 
dc.identifier.epage675
 
dc.identifier.issn0948-6143
2013 Impact Factor: 2.927
 
dc.identifier.issue6
 
dc.identifier.pmid22038040
 
dc.identifier.scopuseid_2-s2.0-84855247292
 
dc.identifier.spage663
 
dc.identifier.urihttp://hdl.handle.net/10722/169588
 
dc.identifier.volume136
 
dc.languageeng
 
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
 
dc.publisher.placeGermany
 
dc.relation.ispartofHistochemistry and Cell Biology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshAntibodies, Monoclonal - Pharmacology
 
dc.subject.meshApoptosis - Drug Effects
 
dc.subject.meshCell Proliferation - Drug Effects
 
dc.subject.meshCells, Cultured
 
dc.subject.meshComputer Simulation
 
dc.subject.meshGene Expression Regulation - Drug Effects
 
dc.subject.meshHedgehog Proteins - Antagonists & Inhibitors - Genetics - Metabolism
 
dc.subject.meshHumans
 
dc.subject.meshImmunohistochemistry
 
dc.subject.meshIn Situ Hybridization
 
dc.subject.meshMale
 
dc.subject.meshMice
 
dc.subject.meshMicroarray Analysis
 
dc.subject.meshPalate - Growth & Development
 
dc.subject.meshReal-Time Polymerase Chain Reaction
 
dc.subject.meshSignal Transduction
 
dc.titleShh signaling is essential for rugae morphogenesis in mice
 
dc.typeArticle
 
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<contributor.author>Kang, DW</contributor.author>
<contributor.author>Choi, BJ</contributor.author>
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Author Affiliations
  1. Yonsei University
  2. Osaka University