File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The forkhead transcription factor Foxc2 stimulates osteoblast differentiation

TitleThe forkhead transcription factor Foxc2 stimulates osteoblast differentiation
Authors
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2009, v. 386 n. 3, p. 532-536 How to Cite?
AbstractThe forkhead box C2 (Foxc2) protein is a member of the family of winged helix/forkhead transcription factors. Foxc2-deficient mice display defective formation of the aortic arches, multiple craniofacial bones, and vertebral columns. To investigate the role of Foxc2 in osteoblast differentiation, DNA containing Foxc2 was transfected into the developing cranial suture mesenchymal cells by electroporation. Compared to the controls, alkaline phosphatase (ALP) and bone sialoprotein were expressed strongly in suture mesenchymal cells in the Foxc2 overexpressed calvaria. After Foxc2-siRNA transfection, ALP staining was rarely observed in the suture mesenchyme and adjacent parietal bone of the calvaria. Meanwhile, overexpression of Foxc2 increased protein levels of β-catenin and stimulated TCF/LEF transcriptional activity. The protein kinase A inhibitor H-89 suppressed Foxc2-mediated increases in TCF/LEF transcriptional activity (-40%, P < 0.01). In conclusion, our results demonstrated that Foxc2 stimulated osteoblast differentiation of mesenchymal cells and preosteoblasts. Activation of canonical Wnt-β-catenin signals might be involved in the Foxc2-mediated stimulation of osteoblast differentiation. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/169561
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKim, SHen_US
dc.contributor.authorCho, KWen_US
dc.contributor.authorChoi, HSen_US
dc.contributor.authorPark, SJen_US
dc.contributor.authorRhee, Yen_US
dc.contributor.authorJung, HSen_US
dc.contributor.authorLim, SKen_US
dc.date.accessioned2012-10-25T04:52:53Z-
dc.date.available2012-10-25T04:52:53Z-
dc.date.issued2009en_US
dc.identifier.citationBiochemical And Biophysical Research Communications, 2009, v. 386 n. 3, p. 532-536en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/169561-
dc.description.abstractThe forkhead box C2 (Foxc2) protein is a member of the family of winged helix/forkhead transcription factors. Foxc2-deficient mice display defective formation of the aortic arches, multiple craniofacial bones, and vertebral columns. To investigate the role of Foxc2 in osteoblast differentiation, DNA containing Foxc2 was transfected into the developing cranial suture mesenchymal cells by electroporation. Compared to the controls, alkaline phosphatase (ALP) and bone sialoprotein were expressed strongly in suture mesenchymal cells in the Foxc2 overexpressed calvaria. After Foxc2-siRNA transfection, ALP staining was rarely observed in the suture mesenchyme and adjacent parietal bone of the calvaria. Meanwhile, overexpression of Foxc2 increased protein levels of β-catenin and stimulated TCF/LEF transcriptional activity. The protein kinase A inhibitor H-89 suppressed Foxc2-mediated increases in TCF/LEF transcriptional activity (-40%, P < 0.01). In conclusion, our results demonstrated that Foxc2 stimulated osteoblast differentiation of mesenchymal cells and preosteoblasts. Activation of canonical Wnt-β-catenin signals might be involved in the Foxc2-mediated stimulation of osteoblast differentiation. © 2009 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Lineen_US
dc.subject.meshForkhead Transcription Factors - Genetics - Physiologyen_US
dc.subject.meshMesenchymal Stem Cells - Cytology - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshOrgan Culture Techniquesen_US
dc.subject.meshOsteoblasts - Cytology - Physiologyen_US
dc.subject.meshRna, Small Interfering - Geneticsen_US
dc.subject.meshSkull - Cytologyen_US
dc.subject.meshWnt Proteins - Metabolismen_US
dc.subject.meshBeta Catenin - Metabolismen_US
dc.titleThe forkhead transcription factor Foxc2 stimulates osteoblast differentiationen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.bbrc.2009.06.071en_US
dc.identifier.pmid19540201-
dc.identifier.scopuseid_2-s2.0-67650229581en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67650229581&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume386en_US
dc.identifier.issue3en_US
dc.identifier.spage532en_US
dc.identifier.epage536en_US
dc.identifier.isiWOS:000268262100023-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKim, SH=34769729600en_US
dc.identifier.scopusauthoridCho, KW=7403956665en_US
dc.identifier.scopusauthoridChoi, HS=8609345400en_US
dc.identifier.scopusauthoridPark, SJ=7501829452en_US
dc.identifier.scopusauthoridRhee, Y=7102441193en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.scopusauthoridLim, SK=7404080753en_US
dc.identifier.citeulike5000011-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats