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Article: Capacity of dental pulp differentiation in mouse molars as demonstrated by allogenic tooth transplantation

TitleCapacity of dental pulp differentiation in mouse molars as demonstrated by allogenic tooth transplantation
Authors
Issue Date2008
PublisherHistochemical Society. The Journal's web site is located at http://intl.jhc.org
Citation
Journal Of Histochemistry And Cytochemistry, 2008, v. 56 n. 12, p. 1075-1086 How to Cite?
Abstract
Dental pulp elaborates both bone and dentin under pathological conditions such as tooth replantation/transplantation. This study aims to clarify the capability of dental pulp to elaborate bone tissue in addition to dentin by allogenic tooth transplantation using immunohistochemistry and histochemistry. After extraction of the molars of 3-week-old mice, the roots and pulp floor were resected and immediately allografted into the sublingual region in a littermate. In addition, we studied the contribution of donor and host cells to the regenerated pulp tissue using a combination of allogenic tooth transplantation and lacZ transgenic ROSA26 mice. On Days 5-7, tubular dentin formation started next to the preexisting dentin at the pulp horn where nestin-positive odontoblast-like cells were arranged. Until Day 14, bone-like tissue formation occurred in the pulp chamber, where intense tartrate-resistant acid phosphatase-positive cells appeared. Furthermore, allogenic transplantation using ROSA26 mice clearly showed that both donor and host cells differentiated into osteoblast-like cells with the assistance of osteoclast-lineage cells, whereas newly differentiated odontoblasts were exclusively derived from donor cells. These results suggest that the odontoblast and osteoblast lineage cells reside in the dental pulp and that both donor and host cells contribute to bone-like tissue formation in the regenerated pulp tissue. © The Histochemical Society, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/169552
ISSN
2013 Impact Factor: 2.403
2013 SCImago Journal Rankings: 1.209
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTakamori, Yen_US
dc.contributor.authorSuzuki, Hen_US
dc.contributor.authorNakakuraOhshima, Ken_US
dc.contributor.authorCai, Jen_US
dc.contributor.authorCho, SWen_US
dc.contributor.authorJung, HSen_US
dc.contributor.authorOhshima, Hen_US
dc.date.accessioned2012-10-25T04:52:48Z-
dc.date.available2012-10-25T04:52:48Z-
dc.date.issued2008en_US
dc.identifier.citationJournal Of Histochemistry And Cytochemistry, 2008, v. 56 n. 12, p. 1075-1086en_US
dc.identifier.issn0022-1554en_US
dc.identifier.urihttp://hdl.handle.net/10722/169552-
dc.description.abstractDental pulp elaborates both bone and dentin under pathological conditions such as tooth replantation/transplantation. This study aims to clarify the capability of dental pulp to elaborate bone tissue in addition to dentin by allogenic tooth transplantation using immunohistochemistry and histochemistry. After extraction of the molars of 3-week-old mice, the roots and pulp floor were resected and immediately allografted into the sublingual region in a littermate. In addition, we studied the contribution of donor and host cells to the regenerated pulp tissue using a combination of allogenic tooth transplantation and lacZ transgenic ROSA26 mice. On Days 5-7, tubular dentin formation started next to the preexisting dentin at the pulp horn where nestin-positive odontoblast-like cells were arranged. Until Day 14, bone-like tissue formation occurred in the pulp chamber, where intense tartrate-resistant acid phosphatase-positive cells appeared. Furthermore, allogenic transplantation using ROSA26 mice clearly showed that both donor and host cells differentiated into osteoblast-like cells with the assistance of osteoclast-lineage cells, whereas newly differentiated odontoblasts were exclusively derived from donor cells. These results suggest that the odontoblast and osteoblast lineage cells reside in the dental pulp and that both donor and host cells contribute to bone-like tissue formation in the regenerated pulp tissue. © The Histochemical Society, Inc.en_US
dc.languageengen_US
dc.publisherHistochemical Society. The Journal's web site is located at http://intl.jhc.orgen_US
dc.relation.ispartofJournal of Histochemistry and Cytochemistryen_US
dc.subject.meshAcid Phosphatase - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshDental Pulp - Cytology - Metabolismen_US
dc.subject.meshHistocytochemistryen_US
dc.subject.meshIntermediate Filament Proteins - Metabolismen_US
dc.subject.meshIsoenzymes - Metabolismen_US
dc.subject.meshLac Operonen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Icren_US
dc.subject.meshMice, Transgenicen_US
dc.subject.meshMolar - Cytology - Metabolism - Transplantationen_US
dc.subject.meshMouth Flooren_US
dc.subject.meshNerve Tissue Proteins - Metabolismen_US
dc.subject.meshOsteopontin - Metabolismen_US
dc.subject.meshTransplantation, Homologousen_US
dc.titleCapacity of dental pulp differentiation in mouse molars as demonstrated by allogenic tooth transplantationen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1369/jhc.2008.951558en_US
dc.identifier.pmid18765839en_US
dc.identifier.scopuseid_2-s2.0-56449123872en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-56449123872&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume56en_US
dc.identifier.issue12en_US
dc.identifier.spage1075en_US
dc.identifier.epage1086en_US
dc.identifier.isiWOS:000261041500003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridTakamori, Y=36922578200en_US
dc.identifier.scopusauthoridSuzuki, H=8740460700en_US
dc.identifier.scopusauthoridNakakuraOhshima, K=6602464566en_US
dc.identifier.scopusauthoridCai, J=9246458800en_US
dc.identifier.scopusauthoridCho, SW=32967447200en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.scopusauthoridOhshima, H=7202879991en_US

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