Article: Capacity of dental pulp differentiation in mouse molars as demonstrated by allogenic tooth transplantation

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TitleCapacity of dental pulp differentiation in mouse molars as demonstrated by allogenic tooth transplantation
AuthorsTakamori, Y2
Suzuki, H2
NakakuraOhshima, K2
Cai, J1
Cho, SW1
Jung, HS1
Ohshima, H2
Issue Date2008
PublisherHistochemical Society. The Journal's web site is located at http://intl.jhc.org
CitationJournal Of Histochemistry And Cytochemistry, 2008, v. 56 n. 12, p. 1075-1086 [How to Cite?]
DOI: http://dx.doi.org/10.1369/jhc.2008.951558
AbstractDental pulp elaborates both bone and dentin under pathological conditions such as tooth replantation/transplantation. This study aims to clarify the capability of dental pulp to elaborate bone tissue in addition to dentin by allogenic tooth transplantation using immunohistochemistry and histochemistry. After extraction of the molars of 3-week-old mice, the roots and pulp floor were resected and immediately allografted into the sublingual region in a littermate. In addition, we studied the contribution of donor and host cells to the regenerated pulp tissue using a combination of allogenic tooth transplantation and lacZ transgenic ROSA26 mice. On Days 5-7, tubular dentin formation started next to the preexisting dentin at the pulp horn where nestin-positive odontoblast-like cells were arranged. Until Day 14, bone-like tissue formation occurred in the pulp chamber, where intense tartrate-resistant acid phosphatase-positive cells appeared. Furthermore, allogenic transplantation using ROSA26 mice clearly showed that both donor and host cells differentiated into osteoblast-like cells with the assistance of osteoclast-lineage cells, whereas newly differentiated odontoblasts were exclusively derived from donor cells. These results suggest that the odontoblast and osteoblast lineage cells reside in the dental pulp and that both donor and host cells contribute to bone-like tissue formation in the regenerated pulp tissue. © The Histochemical Society, Inc.
ISSN0022-1554
2011 Impact Factor: 2.725
2011 SCImago Journal Rankings: 0.283
DOIhttp://dx.doi.org/10.1369/jhc.2008.951558
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorTakamori, Y
dc.contributor.authorSuzuki, H
dc.contributor.authorNakakuraOhshima, K
dc.contributor.authorCai, J
dc.contributor.authorCho, SW
dc.contributor.authorJung, HS
dc.contributor.authorOhshima, H
dc.date.accessioned2012-10-25T04:52:48Z
dc.date.available2012-10-25T04:52:48Z
dc.date.issued2008
dc.description.abstractDental pulp elaborates both bone and dentin under pathological conditions such as tooth replantation/transplantation. This study aims to clarify the capability of dental pulp to elaborate bone tissue in addition to dentin by allogenic tooth transplantation using immunohistochemistry and histochemistry. After extraction of the molars of 3-week-old mice, the roots and pulp floor were resected and immediately allografted into the sublingual region in a littermate. In addition, we studied the contribution of donor and host cells to the regenerated pulp tissue using a combination of allogenic tooth transplantation and lacZ transgenic ROSA26 mice. On Days 5-7, tubular dentin formation started next to the preexisting dentin at the pulp horn where nestin-positive odontoblast-like cells were arranged. Until Day 14, bone-like tissue formation occurred in the pulp chamber, where intense tartrate-resistant acid phosphatase-positive cells appeared. Furthermore, allogenic transplantation using ROSA26 mice clearly showed that both donor and host cells differentiated into osteoblast-like cells with the assistance of osteoclast-lineage cells, whereas newly differentiated odontoblasts were exclusively derived from donor cells. These results suggest that the odontoblast and osteoblast lineage cells reside in the dental pulp and that both donor and host cells contribute to bone-like tissue formation in the regenerated pulp tissue. © The Histochemical Society, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Histochemistry And Cytochemistry, 2008, v. 56 n. 12, p. 1075-1086 [How to Cite?]
DOI: http://dx.doi.org/10.1369/jhc.2008.951558
dc.identifier.doihttp://dx.doi.org/10.1369/jhc.2008.951558
dc.identifier.epage1086
dc.identifier.issn0022-1554
2011 Impact Factor: 2.725
2011 SCImago Journal Rankings: 0.283
dc.identifier.issue12
dc.identifier.pmid18765839
dc.identifier.scopuseid_2-s2.0-56449123872
dc.identifier.spage1075
dc.identifier.urihttp://hdl.handle.net/10722/169552
dc.identifier.volume56
dc.languageeng
dc.publisherHistochemical Society. The Journal's web site is located at http://intl.jhc.org
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Histochemistry and Cytochemistry
dc.relation.referencesReferences in Scopus
dc.subject.meshAcid Phosphatase - Metabolism
dc.subject.meshAnimals
dc.subject.meshCell Differentiation
dc.subject.meshCell Proliferation
dc.subject.meshDental Pulp - Cytology - Metabolism
dc.subject.meshHistocytochemistry
dc.subject.meshIntermediate Filament Proteins - Metabolism
dc.subject.meshIsoenzymes - Metabolism
dc.subject.meshLac Operon
dc.subject.meshMice
dc.subject.meshMice, Inbred Icr
dc.subject.meshMice, Transgenic
dc.subject.meshMolar - Cytology - Metabolism - Transplantation
dc.subject.meshMouth Floor
dc.subject.meshNerve Tissue Proteins - Metabolism
dc.subject.meshOsteopontin - Metabolism
dc.subject.meshTransplantation, Homologous
dc.titleCapacity of dental pulp differentiation in mouse molars as demonstrated by allogenic tooth transplantation
dc.typeArticle
Author Affiliations
  1. Yonsei University
  2. Niigata University School of Medicine