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Article: Localization of putative stem cells in dental epithelium and their association with Notch and FGF signaling

TitleLocalization of putative stem cells in dental epithelium and their association with Notch and FGF signaling
Authors
Issue Date1999
PublisherRockefeller University Press. The Journal's web site is located at http://www.jcb.org
Citation
Journal Of Cell Biology, 1999, v. 147 n. 1, p. 105-120 How to Cite?
AbstractThe continuously growing mouse incisor is an excellent model to analyze the mechanisms for stem cell lineage. We designed an organ culture method for the apical end of the incisor and analyzed the epithelial cell lineage by 5- bromo-2'-deoxyuridine and DiI labeling. Our results indicate that stem cells reside in the cervical loop epithelium consisting of a central core of stellate reticulum cells surrounded by a layer of basal epithelial cells, and that they give rise to transit-amplifying progeny differentiating into enamel forming ameloblasts. We identified slowly dividing cells among the Notch1- expressing stellate reticulum cells in specific locations near the basal epithelial cells expressing lunatic fringe, a secretory molecule modulating Notch signaling. It is known from tissue recombination studies that in the mouse incisor the mesenchyme regulates the continuous growth of epithelium. Expression of Fgf-3 and Fgf-10 were restricted to the mesenchyme underlying the basal epithelial cells and the transit-amplifying cells expressing their receptors Fgfr1b and Fgfr2b. When FGF-10 protein was applied with beads on the cultured cervical loop epithelium it stimulated cell proliferation as well as expression of lunatic fringe. We present a model in which FGF signaling from the mesenchyme regulates the Notch pathway in dental epithelial stem cells via stimulation of lunatic fringe expression and, thereby, has a central role in coupling the mitogenesis and fate decision of stem cells.
Persistent Identifierhttp://hdl.handle.net/10722/169534
ISSN
2015 Impact Factor: 8.717
2015 SCImago Journal Rankings: 7.923
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHarada, Hen_US
dc.contributor.authorKettunen, Pen_US
dc.contributor.authorJung, HSen_US
dc.contributor.authorMustonen, Ten_US
dc.contributor.authorWang, YAen_US
dc.contributor.authorThesleff, Ien_US
dc.date.accessioned2012-10-25T04:52:36Z-
dc.date.available2012-10-25T04:52:36Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of Cell Biology, 1999, v. 147 n. 1, p. 105-120en_US
dc.identifier.issn0021-9525en_US
dc.identifier.urihttp://hdl.handle.net/10722/169534-
dc.description.abstractThe continuously growing mouse incisor is an excellent model to analyze the mechanisms for stem cell lineage. We designed an organ culture method for the apical end of the incisor and analyzed the epithelial cell lineage by 5- bromo-2'-deoxyuridine and DiI labeling. Our results indicate that stem cells reside in the cervical loop epithelium consisting of a central core of stellate reticulum cells surrounded by a layer of basal epithelial cells, and that they give rise to transit-amplifying progeny differentiating into enamel forming ameloblasts. We identified slowly dividing cells among the Notch1- expressing stellate reticulum cells in specific locations near the basal epithelial cells expressing lunatic fringe, a secretory molecule modulating Notch signaling. It is known from tissue recombination studies that in the mouse incisor the mesenchyme regulates the continuous growth of epithelium. Expression of Fgf-3 and Fgf-10 were restricted to the mesenchyme underlying the basal epithelial cells and the transit-amplifying cells expressing their receptors Fgfr1b and Fgfr2b. When FGF-10 protein was applied with beads on the cultured cervical loop epithelium it stimulated cell proliferation as well as expression of lunatic fringe. We present a model in which FGF signaling from the mesenchyme regulates the Notch pathway in dental epithelial stem cells via stimulation of lunatic fringe expression and, thereby, has a central role in coupling the mitogenesis and fate decision of stem cells.en_US
dc.languageengen_US
dc.publisherRockefeller University Press. The Journal's web site is located at http://www.jcb.orgen_US
dc.relation.ispartofJournal of Cell Biologyen_US
dc.subject.meshAmeloblasts - Cytology - Drug Effects - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium-Binding Proteinsen_US
dc.subject.meshCell Differentiation - Drug Effectsen_US
dc.subject.meshCell Division - Drug Effectsen_US
dc.subject.meshCell Lineage - Drug Effectsen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshEpithelial Cells - Drug Effects - Metabolismen_US
dc.subject.meshFibroblast Growth Factor 10en_US
dc.subject.meshFibroblast Growth Factor 3en_US
dc.subject.meshFibroblast Growth Factors - Metabolism - Pharmacologyen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshGlycosyltransferasesen_US
dc.subject.meshIncisor - Cytology - Drug Effects - Metabolismen_US
dc.subject.meshIntercellular Signaling Peptides And Proteinsen_US
dc.subject.meshMembrane Proteins - Genetics - Physiologyen_US
dc.subject.meshMesoderm - Drug Effects - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Strainsen_US
dc.subject.meshProteins - Geneticsen_US
dc.subject.meshProto-Oncogene Proteins - Metabolismen_US
dc.subject.meshRna, Messenger - Analysis - Geneticsen_US
dc.subject.meshReceptors, Fibroblast Growth Factor - Metabolismen_US
dc.subject.meshReceptors, Notchen_US
dc.subject.meshRegenerationen_US
dc.subject.meshSignal Transduction - Drug Effectsen_US
dc.subject.meshStem Cells - Cytology - Drug Effects - Metabolismen_US
dc.subject.meshXenopus Proteinsen_US
dc.titleLocalization of putative stem cells in dental epithelium and their association with Notch and FGF signalingen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1083/jcb.147.1.105en_US
dc.identifier.pmid10508859-
dc.identifier.scopuseid_2-s2.0-0033523757en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033523757&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume147en_US
dc.identifier.issue1en_US
dc.identifier.spage105en_US
dc.identifier.epage120en_US
dc.identifier.isiWOS:000082953900011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHarada, H=7402619046en_US
dc.identifier.scopusauthoridKettunen, P=35902540000en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.scopusauthoridMustonen, T=6603088419en_US
dc.identifier.scopusauthoridWang, YA=18038903300en_US
dc.identifier.scopusauthoridThesleff, I=7005319521en_US

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