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Article: Luminescent detection of DNA-binding proteins

TitleLuminescent detection of DNA-binding proteins
Authors
Issue Date2012
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 2012, v. 40 n. 3, p. 941-955 How to Cite?
AbstractTranscription factors play a central role in cell development, differentiation and growth in biological systems due to their ability to regulate gene expression by binding to specific DNA sequences within the nucleus. The dysregulation of transcription factor signaling has been implicated in the pathogenesis of a number of cancers, developmental disorders, inflammation and autoimmunity. There is thus a high demand for convenient high-throughput methodologies able to detect sequence-specific DNA-binding proteins and monitor their DNA-binding activities. Traditional approaches for protein detection include gel mobility shift assays, DNA footprinting and enzyme-linked immunosorbent assays (ELISAs) which tend to be tedious, time-consuming, and may necessitate the use of radiographic labeling. By contrast, luminescence technologies offer the potential for rapid, sensitive and low-cost detection that are amenable to high-throughput and real-time analysis. The discoveries of molecular beacons and aptamers have spearheaded the development of new luminescent methodologies for the detection of proteins over the last decade. We survey here recent advances in the development of luminescent detection methods for DNA-binding proteins, including those based on molecular beacons, aptamer beacons, label-free techniques and exonuclease protection. © 2011 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/168642
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, CHen_US
dc.contributor.authorChan, DSHen_US
dc.contributor.authorHe, HZen_US
dc.contributor.authorCheng, Zen_US
dc.contributor.authorYang, Hen_US
dc.contributor.authorMa, DLen_US
dc.date.accessioned2012-10-08T03:23:52Z-
dc.date.available2012-10-08T03:23:52Z-
dc.date.issued2012en_US
dc.identifier.citationNucleic Acids Research, 2012, v. 40 n. 3, p. 941-955en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://hdl.handle.net/10722/168642-
dc.description.abstractTranscription factors play a central role in cell development, differentiation and growth in biological systems due to their ability to regulate gene expression by binding to specific DNA sequences within the nucleus. The dysregulation of transcription factor signaling has been implicated in the pathogenesis of a number of cancers, developmental disorders, inflammation and autoimmunity. There is thus a high demand for convenient high-throughput methodologies able to detect sequence-specific DNA-binding proteins and monitor their DNA-binding activities. Traditional approaches for protein detection include gel mobility shift assays, DNA footprinting and enzyme-linked immunosorbent assays (ELISAs) which tend to be tedious, time-consuming, and may necessitate the use of radiographic labeling. By contrast, luminescence technologies offer the potential for rapid, sensitive and low-cost detection that are amenable to high-throughput and real-time analysis. The discoveries of molecular beacons and aptamers have spearheaded the development of new luminescent methodologies for the detection of proteins over the last decade. We survey here recent advances in the development of luminescent detection methods for DNA-binding proteins, including those based on molecular beacons, aptamer beacons, label-free techniques and exonuclease protection. © 2011 The Author(s).en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.subject.meshAptamers, Nucleotide - Chemistryen_US
dc.subject.meshDna-Binding Proteins - Analysisen_US
dc.subject.meshLuminescent Measurementsen_US
dc.subject.meshNuclease Protection Assaysen_US
dc.subject.meshOligonucleotide Probesen_US
dc.titleLuminescent detection of DNA-binding proteinsen_US
dc.typeArticleen_US
dc.identifier.emailLeung, CH:duncanl@hkucc.hku.hken_US
dc.identifier.emailMa, DL:edmondma@hku.hken_US
dc.identifier.authorityLeung, CH=rp00730en_US
dc.identifier.authorityMa, DL=rp00760en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/nar/gkr763en_US
dc.identifier.pmid21967849-
dc.identifier.scopuseid_2-s2.0-84863116694en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863116694&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume40en_US
dc.identifier.issue3en_US
dc.identifier.spage941en_US
dc.identifier.epage955en_US
dc.identifier.isiWOS:000300422400010-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLeung, CH=7402612570en_US
dc.identifier.scopusauthoridChan, DSH=35285471900en_US
dc.identifier.scopusauthoridHe, HZ=35995462000en_US
dc.identifier.scopusauthoridCheng, Z=37039284500en_US
dc.identifier.scopusauthoridYang, H=36653320200en_US
dc.identifier.scopusauthoridMa, DL=7402075538en_US

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