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Article: Energy and entropy effects in dissociation of peptide radical anions

TitleEnergy and entropy effects in dissociation of peptide radical anions
Authors
KeywordsActivation Entropy
Peptide Radical Anion
Rrkm Modeling
Side Chain Loss
Surface-Induced Dissociation
Threshold Energy
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijms
Citation
International Journal Of Mass Spectrometry, 2012, v. 316-318, p. 251-258 How to Cite?
Abstract
Time- and collision energy-resolved surface-induced dissociation (SID) of peptide radical anions was studied using a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) configured for SID experiments. Peptide radical cations and anions were produced by gas-phase fragmentation of Co III(salen)-peptide complexes. The effect of the charge, radical, and the presence of a basic residue on the energetics and dynamics of dissociation of peptide ions was examined using RVYIHPF (1) and HVYIHPF (2) as model systems. Comparison of the survival curves of [M+H] +, [M-H] -, M +, and [M-2H] - ions of these precursors demonstrated that even-electron ions are more stable toward fragmentation than their odd-electron counterparts. RRKM modeling of the experimental data demonstrated that the lower stability of the positive radicals is mainly attributed to lower dissociation thresholds while entropy effects are responsible the relative instability of the negative radicals. Substitution of arginine with less basic histidine residue has a strong destabilizing effect on the [M+H] + ions and a measurable stabilizing effect on the odd-electron ions. Lower threshold energies for dissociation of both positive and negative radicals of 1 are attributed to the presence of lower-energy dissociation pathways that are most likely promoted by the presence of arginine. © 2011 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/168637
ISSN
2013 Impact Factor: 2.227
2013 SCImago Journal Rankings: 0.886
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLaskin, Jen_US
dc.contributor.authorYang, Zen_US
dc.contributor.authorLam, Cen_US
dc.contributor.authorChu, IKen_US
dc.date.accessioned2012-10-08T03:23:48Z-
dc.date.available2012-10-08T03:23:48Z-
dc.date.issued2012en_US
dc.identifier.citationInternational Journal Of Mass Spectrometry, 2012, v. 316-318, p. 251-258en_US
dc.identifier.issn1387-3806en_US
dc.identifier.urihttp://hdl.handle.net/10722/168637-
dc.description.abstractTime- and collision energy-resolved surface-induced dissociation (SID) of peptide radical anions was studied using a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) configured for SID experiments. Peptide radical cations and anions were produced by gas-phase fragmentation of Co III(salen)-peptide complexes. The effect of the charge, radical, and the presence of a basic residue on the energetics and dynamics of dissociation of peptide ions was examined using RVYIHPF (1) and HVYIHPF (2) as model systems. Comparison of the survival curves of [M+H] +, [M-H] -, M +, and [M-2H] - ions of these precursors demonstrated that even-electron ions are more stable toward fragmentation than their odd-electron counterparts. RRKM modeling of the experimental data demonstrated that the lower stability of the positive radicals is mainly attributed to lower dissociation thresholds while entropy effects are responsible the relative instability of the negative radicals. Substitution of arginine with less basic histidine residue has a strong destabilizing effect on the [M+H] + ions and a measurable stabilizing effect on the odd-electron ions. Lower threshold energies for dissociation of both positive and negative radicals of 1 are attributed to the presence of lower-energy dissociation pathways that are most likely promoted by the presence of arginine. © 2011 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijmsen_US
dc.relation.ispartofInternational Journal of Mass Spectrometryen_US
dc.subjectActivation Entropyen_US
dc.subjectPeptide Radical Anionen_US
dc.subjectRrkm Modelingen_US
dc.subjectSide Chain Lossen_US
dc.subjectSurface-Induced Dissociationen_US
dc.subjectThreshold Energyen_US
dc.titleEnergy and entropy effects in dissociation of peptide radical anionsen_US
dc.typeArticleen_US
dc.identifier.emailChu, IK:ivankchu@hku.hken_US
dc.identifier.authorityChu, IK=rp00683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ijms.2012.01.006en_US
dc.identifier.scopuseid_2-s2.0-84862789145en_US
dc.identifier.hkuros208683-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862789145&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume316-318en_US
dc.identifier.spage251en_US
dc.identifier.epage258en_US
dc.identifier.isiWOS:000304507000033-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLaskin, J=7102409836en_US
dc.identifier.scopusauthoridYang, Z=7405435606en_US
dc.identifier.scopusauthoridLam, C=7402990888en_US
dc.identifier.scopusauthoridChu, IK=7103327484en_US
dc.identifier.citeulike10267519-

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