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Article: Mixture of fibroblasts and adipose tissue-derived stem cells can improve epidermal morphogenesis of tissue-engineered skin
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TitleMixture of fibroblasts and adipose tissue-derived stem cells can improve epidermal morphogenesis of tissue-engineered skin
 
AuthorsLu, W3
Yu, J2
Zhang, Y3
Ji, K3
Zhou, Y3
Li, Y3
Deng, Z1
Jin, Y3
 
KeywordsAdipose Tissue-Derived Stem Cells
Dermal Fibroblasts
Epidermal Morphogenesis
Keratinocytes
Skin Tissue Engineering
 
Issue Date2012
 
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/CTO
 
CitationCells Tissues Organs, 2012, v. 195 n. 3, p. 197-206 [How to Cite?]
DOI: http://dx.doi.org/10.1159/000324921
 
AbstractMany studies demonstrate that the type of adjacent mesenchymal cells can affect epidermal morphogenesis of bilayered tissue-engineered skin. However, whether a mixture of different mesenchymal cell types can improve epidermal morphogenesis of bioengineered skin remains unknown. In this study, keratinocytes, dermal fibroblasts and adipose tissue-derived stem cells (ADSCs) were isolated and purified from human skin and subcutaneous fat. Conditioned medium generated from a mixture of dermal fibroblasts and ADSCs at the ratio of 1:1 was superior to that from fibroblasts or ADSCs alone in promoting keratinocyte proliferation, as indicated by MTT assay. Furthermore, ELISA results showed that the cytokine levels of human hepatocyte growth factor and keratinocyte growth factor (also known as FGF7) in the mixed fibroblasts/ADSC group were higher than those in the ADSC or dermal fibroblasts group. To examine the potential roles of mixed fibroblasts and ADSCs on epidermal morphogenesis, a three-dimensional tissue engineered skin system was applied. Histological analyses demonstrated that keratinocytes proliferated extensively over the mixture of fibroblasts and ADSCs, and formed a thick epidermal layer with well-differentiated structures. Keratin 10 (epidermal differentiation marker) was expressed in the suprabasal layer of bilayered tissue-engineered skin in the mixed fibroblasts and ADSCs group. Desmosomes and hemidesmosomes were detected in the newly formed epidermis by transmission electron microscopy analysis. Together, these findings revealed for the first time that a mixture of fibroblasts and ADSCs in bilayered tissue-engineered skin can improve epidermal morphogenesis. Copyright © 2012 S. Karger AG, Basel.
 
ISSN1422-6405
2013 Impact Factor: 2.138
 
DOIhttp://dx.doi.org/10.1159/000324921
 
ISI Accession Number IDWOS:000300641900002
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLu, W
 
dc.contributor.authorYu, J
 
dc.contributor.authorZhang, Y
 
dc.contributor.authorJi, K
 
dc.contributor.authorZhou, Y
 
dc.contributor.authorLi, Y
 
dc.contributor.authorDeng, Z
 
dc.contributor.authorJin, Y
 
dc.date.accessioned2012-10-08T03:21:35Z
 
dc.date.available2012-10-08T03:21:35Z
 
dc.date.issued2012
 
dc.description.abstractMany studies demonstrate that the type of adjacent mesenchymal cells can affect epidermal morphogenesis of bilayered tissue-engineered skin. However, whether a mixture of different mesenchymal cell types can improve epidermal morphogenesis of bioengineered skin remains unknown. In this study, keratinocytes, dermal fibroblasts and adipose tissue-derived stem cells (ADSCs) were isolated and purified from human skin and subcutaneous fat. Conditioned medium generated from a mixture of dermal fibroblasts and ADSCs at the ratio of 1:1 was superior to that from fibroblasts or ADSCs alone in promoting keratinocyte proliferation, as indicated by MTT assay. Furthermore, ELISA results showed that the cytokine levels of human hepatocyte growth factor and keratinocyte growth factor (also known as FGF7) in the mixed fibroblasts/ADSC group were higher than those in the ADSC or dermal fibroblasts group. To examine the potential roles of mixed fibroblasts and ADSCs on epidermal morphogenesis, a three-dimensional tissue engineered skin system was applied. Histological analyses demonstrated that keratinocytes proliferated extensively over the mixture of fibroblasts and ADSCs, and formed a thick epidermal layer with well-differentiated structures. Keratin 10 (epidermal differentiation marker) was expressed in the suprabasal layer of bilayered tissue-engineered skin in the mixed fibroblasts and ADSCs group. Desmosomes and hemidesmosomes were detected in the newly formed epidermis by transmission electron microscopy analysis. Together, these findings revealed for the first time that a mixture of fibroblasts and ADSCs in bilayered tissue-engineered skin can improve epidermal morphogenesis. Copyright © 2012 S. Karger AG, Basel.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationCells Tissues Organs, 2012, v. 195 n. 3, p. 197-206 [How to Cite?]
DOI: http://dx.doi.org/10.1159/000324921
 
dc.identifier.doihttp://dx.doi.org/10.1159/000324921
 
dc.identifier.epage206
 
dc.identifier.isiWOS:000300641900002
 
dc.identifier.issn1422-6405
2013 Impact Factor: 2.138
 
dc.identifier.issue3
 
dc.identifier.pmid21494022
 
dc.identifier.scopuseid_2-s2.0-84857910591
 
dc.identifier.spage197
 
dc.identifier.urihttp://hdl.handle.net/10722/168611
 
dc.identifier.volume195
 
dc.languageeng
 
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/CTO
 
dc.publisher.placeSwitzerland
 
dc.relation.ispartofCells Tissues Organs
 
dc.relation.referencesReferences in Scopus
 
dc.subjectAdipose Tissue-Derived Stem Cells
 
dc.subjectDermal Fibroblasts
 
dc.subjectEpidermal Morphogenesis
 
dc.subjectKeratinocytes
 
dc.subjectSkin Tissue Engineering
 
dc.titleMixture of fibroblasts and adipose tissue-derived stem cells can improve epidermal morphogenesis of tissue-engineered skin
 
dc.typeArticle
 
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<contributor.author>Zhang, Y</contributor.author>
<contributor.author>Ji, K</contributor.author>
<contributor.author>Zhou, Y</contributor.author>
<contributor.author>Li, Y</contributor.author>
<contributor.author>Deng, Z</contributor.author>
<contributor.author>Jin, Y</contributor.author>
<date.accessioned>2012-10-08T03:21:35Z</date.accessioned>
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<subject>Adipose Tissue-Derived Stem Cells</subject>
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Author Affiliations
  1. Xijing Hospital
  2. Nanjing Medical University
  3. The Fourth Military Medical University