Article: Mixture of fibroblasts and adipose tissue-derived stem cells can improve epidermal morphogenesis of tissue-engineered skin

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TitleMixture of fibroblasts and adipose tissue-derived stem cells can improve epidermal morphogenesis of tissue-engineered skin
AuthorsLu, W3
Yu, J2
Zhang, Y3
Ji, K3
Zhou, Y3
Li, Y3
Deng, Z1
Jin, Y3
KeywordsAdipose Tissue-Derived Stem Cells
Dermal Fibroblasts
Epidermal Morphogenesis
Keratinocytes
Skin Tissue Engineering
Issue Date2012
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/CTO
CitationCells Tissues Organs, 2012, v. 195 n. 3, p. 197-206 [How to Cite?]
DOI: http://dx.doi.org/10.1159/000324921
AbstractMany studies demonstrate that the type of adjacent mesenchymal cells can affect epidermal morphogenesis of bilayered tissue-engineered skin. However, whether a mixture of different mesenchymal cell types can improve epidermal morphogenesis of bioengineered skin remains unknown. In this study, keratinocytes, dermal fibroblasts and adipose tissue-derived stem cells (ADSCs) were isolated and purified from human skin and subcutaneous fat. Conditioned medium generated from a mixture of dermal fibroblasts and ADSCs at the ratio of 1:1 was superior to that from fibroblasts or ADSCs alone in promoting keratinocyte proliferation, as indicated by MTT assay. Furthermore, ELISA results showed that the cytokine levels of human hepatocyte growth factor and keratinocyte growth factor (also known as FGF7) in the mixed fibroblasts/ADSC group were higher than those in the ADSC or dermal fibroblasts group. To examine the potential roles of mixed fibroblasts and ADSCs on epidermal morphogenesis, a three-dimensional tissue engineered skin system was applied. Histological analyses demonstrated that keratinocytes proliferated extensively over the mixture of fibroblasts and ADSCs, and formed a thick epidermal layer with well-differentiated structures. Keratin 10 (epidermal differentiation marker) was expressed in the suprabasal layer of bilayered tissue-engineered skin in the mixed fibroblasts and ADSCs group. Desmosomes and hemidesmosomes were detected in the newly formed epidermis by transmission electron microscopy analysis. Together, these findings revealed for the first time that a mixture of fibroblasts and ADSCs in bilayered tissue-engineered skin can improve epidermal morphogenesis. Copyright © 2012 S. Karger AG, Basel.
ISSN1422-6405
2011 Impact Factor: 2.203
2011 SCImago Journal Rankings: 0.196
DOIhttp://dx.doi.org/10.1159/000324921
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLu, W
dc.contributor.authorYu, J
dc.contributor.authorZhang, Y
dc.contributor.authorJi, K
dc.contributor.authorZhou, Y
dc.contributor.authorLi, Y
dc.contributor.authorDeng, Z
dc.contributor.authorJin, Y
dc.date.accessioned2012-10-08T03:21:35Z
dc.date.available2012-10-08T03:21:35Z
dc.date.issued2012
dc.description.abstractMany studies demonstrate that the type of adjacent mesenchymal cells can affect epidermal morphogenesis of bilayered tissue-engineered skin. However, whether a mixture of different mesenchymal cell types can improve epidermal morphogenesis of bioengineered skin remains unknown. In this study, keratinocytes, dermal fibroblasts and adipose tissue-derived stem cells (ADSCs) were isolated and purified from human skin and subcutaneous fat. Conditioned medium generated from a mixture of dermal fibroblasts and ADSCs at the ratio of 1:1 was superior to that from fibroblasts or ADSCs alone in promoting keratinocyte proliferation, as indicated by MTT assay. Furthermore, ELISA results showed that the cytokine levels of human hepatocyte growth factor and keratinocyte growth factor (also known as FGF7) in the mixed fibroblasts/ADSC group were higher than those in the ADSC or dermal fibroblasts group. To examine the potential roles of mixed fibroblasts and ADSCs on epidermal morphogenesis, a three-dimensional tissue engineered skin system was applied. Histological analyses demonstrated that keratinocytes proliferated extensively over the mixture of fibroblasts and ADSCs, and formed a thick epidermal layer with well-differentiated structures. Keratin 10 (epidermal differentiation marker) was expressed in the suprabasal layer of bilayered tissue-engineered skin in the mixed fibroblasts and ADSCs group. Desmosomes and hemidesmosomes were detected in the newly formed epidermis by transmission electron microscopy analysis. Together, these findings revealed for the first time that a mixture of fibroblasts and ADSCs in bilayered tissue-engineered skin can improve epidermal morphogenesis. Copyright © 2012 S. Karger AG, Basel.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCells Tissues Organs, 2012, v. 195 n. 3, p. 197-206 [How to Cite?]
DOI: http://dx.doi.org/10.1159/000324921
dc.identifier.doihttp://dx.doi.org/10.1159/000324921
dc.identifier.epage206
dc.identifier.issn1422-6405
2011 Impact Factor: 2.203
2011 SCImago Journal Rankings: 0.196
dc.identifier.issue3
dc.identifier.scopuseid_2-s2.0-84857910591
dc.identifier.spage197
dc.identifier.urihttp://hdl.handle.net/10722/168611
dc.identifier.volume195
dc.languageeng
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/CTO
dc.publisher.placeSwitzerland
dc.relation.ispartofCells Tissues Organs
dc.relation.referencesReferences in Scopus
dc.subjectAdipose Tissue-Derived Stem Cells
dc.subjectDermal Fibroblasts
dc.subjectEpidermal Morphogenesis
dc.subjectKeratinocytes
dc.subjectSkin Tissue Engineering
dc.titleMixture of fibroblasts and adipose tissue-derived stem cells can improve epidermal morphogenesis of tissue-engineered skin
dc.typeArticle
Author Affiliations
  1. Xijing Hospital
  2. Nanjing Medical University
  3. The Fourth Military Medical University