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Article: Mixture of fibroblasts and adipose tissue-derived stem cells can improve epidermal morphogenesis of tissue-engineered skin

TitleMixture of fibroblasts and adipose tissue-derived stem cells can improve epidermal morphogenesis of tissue-engineered skin
Authors
KeywordsAdipose Tissue-Derived Stem Cells
Dermal Fibroblasts
Epidermal Morphogenesis
Keratinocytes
Skin Tissue Engineering
Issue Date2012
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/CTO
Citation
Cells Tissues Organs, 2012, v. 195 n. 3, p. 197-206 How to Cite?
Abstract
Many studies demonstrate that the type of adjacent mesenchymal cells can affect epidermal morphogenesis of bilayered tissue-engineered skin. However, whether a mixture of different mesenchymal cell types can improve epidermal morphogenesis of bioengineered skin remains unknown. In this study, keratinocytes, dermal fibroblasts and adipose tissue-derived stem cells (ADSCs) were isolated and purified from human skin and subcutaneous fat. Conditioned medium generated from a mixture of dermal fibroblasts and ADSCs at the ratio of 1:1 was superior to that from fibroblasts or ADSCs alone in promoting keratinocyte proliferation, as indicated by MTT assay. Furthermore, ELISA results showed that the cytokine levels of human hepatocyte growth factor and keratinocyte growth factor (also known as FGF7) in the mixed fibroblasts/ADSC group were higher than those in the ADSC or dermal fibroblasts group. To examine the potential roles of mixed fibroblasts and ADSCs on epidermal morphogenesis, a three-dimensional tissue engineered skin system was applied. Histological analyses demonstrated that keratinocytes proliferated extensively over the mixture of fibroblasts and ADSCs, and formed a thick epidermal layer with well-differentiated structures. Keratin 10 (epidermal differentiation marker) was expressed in the suprabasal layer of bilayered tissue-engineered skin in the mixed fibroblasts and ADSCs group. Desmosomes and hemidesmosomes were detected in the newly formed epidermis by transmission electron microscopy analysis. Together, these findings revealed for the first time that a mixture of fibroblasts and ADSCs in bilayered tissue-engineered skin can improve epidermal morphogenesis. Copyright © 2012 S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/168611
ISSN
2013 Impact Factor: 2.138
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Wen_US
dc.contributor.authorYu, Jen_US
dc.contributor.authorZhang, Yen_US
dc.contributor.authorJi, Ken_US
dc.contributor.authorZhou, Yen_US
dc.contributor.authorLi, Yen_US
dc.contributor.authorDeng, Zen_US
dc.contributor.authorJin, Yen_US
dc.date.accessioned2012-10-08T03:21:35Z-
dc.date.available2012-10-08T03:21:35Z-
dc.date.issued2012en_US
dc.identifier.citationCells Tissues Organs, 2012, v. 195 n. 3, p. 197-206en_US
dc.identifier.issn1422-6405en_US
dc.identifier.urihttp://hdl.handle.net/10722/168611-
dc.description.abstractMany studies demonstrate that the type of adjacent mesenchymal cells can affect epidermal morphogenesis of bilayered tissue-engineered skin. However, whether a mixture of different mesenchymal cell types can improve epidermal morphogenesis of bioengineered skin remains unknown. In this study, keratinocytes, dermal fibroblasts and adipose tissue-derived stem cells (ADSCs) were isolated and purified from human skin and subcutaneous fat. Conditioned medium generated from a mixture of dermal fibroblasts and ADSCs at the ratio of 1:1 was superior to that from fibroblasts or ADSCs alone in promoting keratinocyte proliferation, as indicated by MTT assay. Furthermore, ELISA results showed that the cytokine levels of human hepatocyte growth factor and keratinocyte growth factor (also known as FGF7) in the mixed fibroblasts/ADSC group were higher than those in the ADSC or dermal fibroblasts group. To examine the potential roles of mixed fibroblasts and ADSCs on epidermal morphogenesis, a three-dimensional tissue engineered skin system was applied. Histological analyses demonstrated that keratinocytes proliferated extensively over the mixture of fibroblasts and ADSCs, and formed a thick epidermal layer with well-differentiated structures. Keratin 10 (epidermal differentiation marker) was expressed in the suprabasal layer of bilayered tissue-engineered skin in the mixed fibroblasts and ADSCs group. Desmosomes and hemidesmosomes were detected in the newly formed epidermis by transmission electron microscopy analysis. Together, these findings revealed for the first time that a mixture of fibroblasts and ADSCs in bilayered tissue-engineered skin can improve epidermal morphogenesis. Copyright © 2012 S. Karger AG, Basel.en_US
dc.languageengen_US
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/CTOen_US
dc.relation.ispartofCells Tissues Organsen_US
dc.subjectAdipose Tissue-Derived Stem Cellsen_US
dc.subjectDermal Fibroblastsen_US
dc.subjectEpidermal Morphogenesisen_US
dc.subjectKeratinocytesen_US
dc.subjectSkin Tissue Engineeringen_US
dc.titleMixture of fibroblasts and adipose tissue-derived stem cells can improve epidermal morphogenesis of tissue-engineered skinen_US
dc.typeArticleen_US
dc.identifier.emailLu, W:luwei@hku.hken_US
dc.identifier.authorityLu, W=rp00754en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1159/000324921en_US
dc.identifier.pmid21494022-
dc.identifier.scopuseid_2-s2.0-84857910591en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84857910591&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume195en_US
dc.identifier.issue3en_US
dc.identifier.spage197en_US
dc.identifier.epage206en_US
dc.identifier.isiWOS:000300641900002-
dc.publisher.placeSwitzerlanden_US
dc.identifier.scopusauthoridLu, W=27868087600en_US
dc.identifier.scopusauthoridYu, J=37082539500en_US
dc.identifier.scopusauthoridZhang, Y=37082390500en_US
dc.identifier.scopusauthoridJi, K=8410095700en_US
dc.identifier.scopusauthoridZhou, Y=37082401400en_US
dc.identifier.scopusauthoridLi, Y=37081087300en_US
dc.identifier.scopusauthoridDeng, Z=15119136700en_US
dc.identifier.scopusauthoridJin, Y=26643271200en_US

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