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Article: A novel late embryogenesis abundant like protein associated with chilling stress in Nicotiana tabacum cv. bright yellow-2 cell suspension culture.

TitleA novel late embryogenesis abundant like protein associated with chilling stress in Nicotiana tabacum cv. bright yellow-2 cell suspension culture.
Authors
Issue Date2011
Citation
Molecular & Cellular Proteomics : Mcp, 2011, v. 10 n. 11, p. M111.010363 How to Cite?
AbstractLow temperature is one of the major abiotic stresses limiting the productivity and geographical distribution of many important crops. To identify proteins associated with chilling stress in Nicotiana tabacum cv. bright yellow-2 (BY-2) cell suspension culture, we utilized a proteomic approach with two-dimensional electrophoresis to compare proteins from samples of treated with or without chilling treatment at 4 °C. One protein specifically more abundant in chilling treated sample was identified and designated as NtLEA7-3. Rapid amplification of cDNA ends gave rise to a full-length NtLEA7-3 cDNA with a complete open reading frame of 1267 bp, encoding a 322 amino acid polypeptide. Homology search and sequence multi-alignment demonstrated that the deduced NtLEA7-3 protein sequence shared a high identity with LEA-like proteins from other plants. Subcellular localization analysis indicated that the NtLEA7-3 was localized exclusively in the nucleus. When the gene was overexpressed in bright yellow-2 cells, the transgenic bright yellow-2 cells show more resistant to chilling stress than the wild-type cells. In addition, transgenic Arabidopsis plants overexpressing the NtLEA7-3 are much more resistant to cold, drought, and salt stresses. Interestingly, the expression of NtLEA7-3 in tobacco was not tissue-specific and induced by chilling, drought and salt stresses. All of these, taken together, suggest that NtLEA7-3 is worthwhile to elucidate the contribution of the proteins to the tolerance mechanism to chilling stress, and can be considered as a potential target for crop genetic improvement in the future.
Persistent Identifierhttp://hdl.handle.net/10722/168610
ISSN
2015 SCImago Journal Rankings: 3.213
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGai, YPen_US
dc.contributor.authorJi, XLen_US
dc.contributor.authorLu, Wen_US
dc.contributor.authorHan, XJen_US
dc.contributor.authorYang, GDen_US
dc.contributor.authorZheng, CCen_US
dc.date.accessioned2012-10-08T03:21:33Z-
dc.date.available2012-10-08T03:21:33Z-
dc.date.issued2011en_US
dc.identifier.citationMolecular & Cellular Proteomics : Mcp, 2011, v. 10 n. 11, p. M111.010363en_US
dc.identifier.issn1535-9484en_US
dc.identifier.urihttp://hdl.handle.net/10722/168610-
dc.description.abstractLow temperature is one of the major abiotic stresses limiting the productivity and geographical distribution of many important crops. To identify proteins associated with chilling stress in Nicotiana tabacum cv. bright yellow-2 (BY-2) cell suspension culture, we utilized a proteomic approach with two-dimensional electrophoresis to compare proteins from samples of treated with or without chilling treatment at 4 °C. One protein specifically more abundant in chilling treated sample was identified and designated as NtLEA7-3. Rapid amplification of cDNA ends gave rise to a full-length NtLEA7-3 cDNA with a complete open reading frame of 1267 bp, encoding a 322 amino acid polypeptide. Homology search and sequence multi-alignment demonstrated that the deduced NtLEA7-3 protein sequence shared a high identity with LEA-like proteins from other plants. Subcellular localization analysis indicated that the NtLEA7-3 was localized exclusively in the nucleus. When the gene was overexpressed in bright yellow-2 cells, the transgenic bright yellow-2 cells show more resistant to chilling stress than the wild-type cells. In addition, transgenic Arabidopsis plants overexpressing the NtLEA7-3 are much more resistant to cold, drought, and salt stresses. Interestingly, the expression of NtLEA7-3 in tobacco was not tissue-specific and induced by chilling, drought and salt stresses. All of these, taken together, suggest that NtLEA7-3 is worthwhile to elucidate the contribution of the proteins to the tolerance mechanism to chilling stress, and can be considered as a potential target for crop genetic improvement in the future.en_US
dc.languageengen_US
dc.relation.ispartofMolecular & cellular proteomics : MCPen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshCell Culture Techniquesen_US
dc.subject.meshCold Temperatureen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPlant Proteins - Genetics - Metabolismen_US
dc.subject.meshPlant Roots - Metabolismen_US
dc.subject.meshProteome - Metabolismen_US
dc.subject.meshSeeds - Cytology - Metabolism - Physiologyen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshStress, Physiologicalen_US
dc.subject.meshTobacco - Cytology - Metabolism - Physiologyen_US
dc.titleA novel late embryogenesis abundant like protein associated with chilling stress in Nicotiana tabacum cv. bright yellow-2 cell suspension culture.en_US
dc.typeArticleen_US
dc.identifier.emailLu, W:luwei@hku.hken_US
dc.identifier.authorityLu, W=rp00754en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1074/mcp.M111.010363-
dc.identifier.pmid21653253-
dc.identifier.pmcidPMC3226408-
dc.identifier.scopuseid_2-s2.0-84857776839en_US
dc.identifier.volume10en_US
dc.identifier.issue11en_US
dc.identifier.spageM111.010363en_US
dc.identifier.isiWOS:000296759400013-
dc.identifier.scopusauthoridGai, YP=24537012700en_US
dc.identifier.scopusauthoridJi, XL=24461801900en_US
dc.identifier.scopusauthoridLu, W=27868087600en_US
dc.identifier.scopusauthoridHan, XJ=54406851800en_US
dc.identifier.scopusauthoridYang, GD=8987732300en_US
dc.identifier.scopusauthoridZheng, CC=7401935024en_US

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