Article: Over-expression of PDGFR-β promotes PDGF-induced proliferation, migration, and angiogenesis of EPCs through PI3K/Akt signaling pathway
| Title | Over-expression of PDGFR-β promotes PDGF-induced proliferation, migration, and angiogenesis of EPCs through PI3K/Akt signaling pathway |
|---|---|
| Authors | Wang, H1 Yin, Y1 Li, W1 Zhao, X1 Yu, Y1 Zhu, J1 Qin, Z1 Wang, Q1 Wang, K1 Lu, W1 Liu, J1 Huang, L1 |
| Issue Date | 2012 |
| Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action |
| Citation | Plos One, 2012, v. 7 n. 2 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0030503 |
| Abstract | The proliferation, migration, and angiogenesis of endothelial progenitor cells (EPCs) play critical roles in postnatal neovascularization and re-endothelialization following vascular injury. Here we evaluated whether the over-expression of platelet-derived growth factor receptor-β (PDGFR-β) can enhance the PDGF-BB-stimulated biological functions of EPCs through the PDGFR-β/phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. We first confirmed the expression of endogenous PDGFR-β and its plasma membrane localization in spleen-derived EPCs. We then demonstrated that the PDGFR-β over-expression in EPCs enhanced the PDGF-BB-induced proliferation, migration, and angiogenesis of EPCs. Using AG1295 (a PDGFR kinase inhibitor), LY294002 (a PI3K inhibitor), and sc-221226 (an Akt inhibitor), we further showed that the PI3K/Akt signaling pathway participates in the PDGF-BB-induced proliferation, migration, and angiogenesis of EPCs. In addition, the PI3K/Akt signaling pathway is required for PDGFR-β over-expression to enhance these PDGF-BB-induced phenotypes. © 2012 Wang et al. |
| ISSN | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 |
| DOI | http://dx.doi.org/10.1371/journal.pone.0030503 |
| References | References in Scopus |
| dc.contributor.author | Wang, H |
|---|---|
| dc.contributor.author | Yin, Y |
| dc.contributor.author | Li, W |
| dc.contributor.author | Zhao, X |
| dc.contributor.author | Yu, Y |
| dc.contributor.author | Zhu, J |
| dc.contributor.author | Qin, Z |
| dc.contributor.author | Wang, Q |
| dc.contributor.author | Wang, K |
| dc.contributor.author | Lu, W |
| dc.contributor.author | Liu, J |
| dc.contributor.author | Huang, L |
| dc.date.accessioned | 2012-10-08T03:21:29Z |
| dc.date.available | 2012-10-08T03:21:29Z |
| dc.date.issued | 2012 |
| dc.description.abstract | The proliferation, migration, and angiogenesis of endothelial progenitor cells (EPCs) play critical roles in postnatal neovascularization and re-endothelialization following vascular injury. Here we evaluated whether the over-expression of platelet-derived growth factor receptor-β (PDGFR-β) can enhance the PDGF-BB-stimulated biological functions of EPCs through the PDGFR-β/phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. We first confirmed the expression of endogenous PDGFR-β and its plasma membrane localization in spleen-derived EPCs. We then demonstrated that the PDGFR-β over-expression in EPCs enhanced the PDGF-BB-induced proliferation, migration, and angiogenesis of EPCs. Using AG1295 (a PDGFR kinase inhibitor), LY294002 (a PI3K inhibitor), and sc-221226 (an Akt inhibitor), we further showed that the PI3K/Akt signaling pathway participates in the PDGF-BB-induced proliferation, migration, and angiogenesis of EPCs. In addition, the PI3K/Akt signaling pathway is required for PDGFR-β over-expression to enhance these PDGF-BB-induced phenotypes. © 2012 Wang et al. |
| dc.description.nature | Link_to_subscribed_fulltext |
| dc.identifier.citation | Plos One, 2012, v. 7 n. 2 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0030503 |
| dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0030503 |
| dc.identifier.issn | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 |
| dc.identifier.issue | 2 |
| dc.identifier.scopus | eid_2-s2.0-84857080131 |
| dc.identifier.uri | http://hdl.handle.net/10722/168607 |
| dc.identifier.volume | 7 |
| dc.language | eng |
| dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action |
| dc.publisher.place | United States |
| dc.relation.ispartof | PLoS ONE |
| dc.relation.references | References in Scopus |
| dc.title | Over-expression of PDGFR-β promotes PDGF-induced proliferation, migration, and angiogenesis of EPCs through PI3K/Akt signaling pathway |
| dc.type | Article |
Author Affiliations
- Third Military Medical University