Article: Ethanolic extract of fructus alpinia oxyphylla protects against 6-hydroxydopamine-induced damage of PC12 cells in vitro and dopaminergic neurons in zebrafish

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TitleEthanolic extract of fructus alpinia oxyphylla protects against 6-hydroxydopamine-induced damage of PC12 cells in vitro and dopaminergic neurons in zebrafish
AuthorsZhang, ZJ1
Cheang, LCV1
Wang, MW1
Li, GH1
Chu, IK1
Lin, ZX1
Lee, SMY1
Issue Date2012
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0272-4340
CitationCellular And Molecular Neurobiology, 2012, v. 32 n. 1, p. 27-40 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10571-011-9731-0
AbstractIn an attempt to understand the neuroprotective effect of Fructus Alpinia oxyphylla (AOE) and to elucidate its underlying mechanism of action, the ethanolic extract of AOE was investigated using zebrafish and PC12 cell models. AOE prevented and restored 6-hydroxydopamine (6-OHDA)-induced dopaminergic (DA) neuron degeneration and attenuated a deficit of locomotor activity in a zebrafish (Danio rerio) model of Parkinson's disease (PD). Treatment with AOE increased the viability of 6-OHDAtreated PC12 cells in vitro in a dose-dependent manner by attenuating cellular apoptosis. However, protocatechuic acid (PCA) and chrysin, two known polyphenol components of AOE, could not reproduce the neuroprotective activity of AOE in the PD zebrafish or PC12 cell models. A mechanistic study found that the protective effect of AOE against 6-OHDA-induced neuronal injury involved antiinflammatory action (down-regulation of gene expression of IL-1β and TNF-α) and anti-oxidative action (inhibition of NO production and iNOS expression in PC12 cells). Moreover, the PI3K-AKT pathway might be part of the mechanism of neuroprotection of AOE. The results of this research are expected to provide a scientific rationale for the use of AOE in the treatment of PD. However, it is important that the active components that contribute to the neuroprotective action of AOE are identified and characterized. © Springer Science+Business Media, LLC 2011.
ISSN0272-4340
2011 Impact Factor: 1.969
2011 SCImago Journal Rankings: 0.148
DOIhttp://dx.doi.org/10.1007/s10571-011-9731-0
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorZhang, ZJ
dc.contributor.authorCheang, LCV
dc.contributor.authorWang, MW
dc.contributor.authorLi, GH
dc.contributor.authorChu, IK
dc.contributor.authorLin, ZX
dc.contributor.authorLee, SMY
dc.date.accessioned2012-10-08T03:21:28Z
dc.date.available2012-10-08T03:21:28Z
dc.date.issued2012
dc.description.abstractIn an attempt to understand the neuroprotective effect of Fructus Alpinia oxyphylla (AOE) and to elucidate its underlying mechanism of action, the ethanolic extract of AOE was investigated using zebrafish and PC12 cell models. AOE prevented and restored 6-hydroxydopamine (6-OHDA)-induced dopaminergic (DA) neuron degeneration and attenuated a deficit of locomotor activity in a zebrafish (Danio rerio) model of Parkinson's disease (PD). Treatment with AOE increased the viability of 6-OHDAtreated PC12 cells in vitro in a dose-dependent manner by attenuating cellular apoptosis. However, protocatechuic acid (PCA) and chrysin, two known polyphenol components of AOE, could not reproduce the neuroprotective activity of AOE in the PD zebrafish or PC12 cell models. A mechanistic study found that the protective effect of AOE against 6-OHDA-induced neuronal injury involved antiinflammatory action (down-regulation of gene expression of IL-1β and TNF-α) and anti-oxidative action (inhibition of NO production and iNOS expression in PC12 cells). Moreover, the PI3K-AKT pathway might be part of the mechanism of neuroprotection of AOE. The results of this research are expected to provide a scientific rationale for the use of AOE in the treatment of PD. However, it is important that the active components that contribute to the neuroprotective action of AOE are identified and characterized. © Springer Science+Business Media, LLC 2011.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCellular And Molecular Neurobiology, 2012, v. 32 n. 1, p. 27-40 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10571-011-9731-0
dc.identifier.citeulike9547239
dc.identifier.doihttp://dx.doi.org/10.1007/s10571-011-9731-0
dc.identifier.epage40
dc.identifier.hkuros208681
dc.identifier.issn0272-4340
2011 Impact Factor: 1.969
2011 SCImago Journal Rankings: 0.148
dc.identifier.issue1
dc.identifier.pmid21744117
dc.identifier.scopuseid_2-s2.0-84856534483
dc.identifier.spage27
dc.identifier.urihttp://hdl.handle.net/10722/168606
dc.identifier.volume32
dc.languageeng
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0272-4340
dc.publisher.placeUnited States
dc.relation.ispartofCellular and Molecular Neurobiology
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshBehavior, Animal - Drug Effects
dc.subject.meshCell Death - Drug Effects
dc.subject.meshCytoprotection - Drug Effects
dc.subject.meshDopaminergic Neurons - Drug Effects - Physiology
dc.subject.meshEmbryo, Nonmammalian
dc.subject.meshEthanol - Pharmacology
dc.subject.meshLarva - Drug Effects - Growth & Development - Physiology
dc.subject.meshLocomotion - Drug Effects
dc.subject.meshOxidopamine - Toxicity
dc.subject.meshPc12 Cells
dc.subject.meshPlant Extracts - Chemistry - Pharmacology
dc.subject.meshRats
dc.subject.meshZebrafish - Embryology - Growth & Development
dc.titleEthanolic extract of fructus alpinia oxyphylla protects against 6-hydroxydopamine-induced damage of PC12 cells in vitro and dopaminergic neurons in zebrafish
dc.typeArticle
Author Affiliations
  1. Chinese University of Hong Kong